1. Caldesmon, calponin and alpha-smooth muscle actin expression in subcultured smooth muscle cells from human airways.
- Author
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Durand-Arczynska W, Marmy N, and Durand J
- Subjects
- Bronchi cytology, Bronchi metabolism, Cell Division, Cells, Cultured, Humans, Immunohistochemistry, Microfilament Proteins, Muscle Proteins metabolism, Muscle, Smooth cytology, Muscle, Smooth metabolism, Respiratory Muscles cytology, Calponins, Actins metabolism, Calcium-Binding Proteins metabolism, Calmodulin-Binding Proteins metabolism, Respiratory Muscles metabolism
- Abstract
Calponin and caldesmon are two proteins considered to play a regulatory role in smooth muscle contraction, which have never previously been found to be expressed in subcultured cells. In the present study, immunocytochemistry and immunoblotting were performed to identify these proteins in smooth muscle cells (SMC) from human bronchi. It was found that human airway SMC, kept in a non-proliferative state, continued to express caldesmon and calponin at least until the 8th passage. The expression of alpha-smooth muscle actin studied under the same conditions was also shown to be preserved in subcultured bronchial SMC.
- Published
- 1993
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