1. Rapid detection of t(15;17)(q24;q21) in acute promyelocytic leukaemia by microwave-assisted fluorescence in situ hybridization.
- Author
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Soriani S, Mura C, Panico AR, Scarpa AM, Recchimuzzo P, Dadati R, Farioli R, De Canal G, Mura MA, and Cesana C
- Subjects
- DNA Probes, DNA, Neoplasm analysis, Female, Gene Rearrangement, Humans, Immunophenotyping, In Situ Hybridization, In Situ Hybridization, Fluorescence, Karyotyping, Male, Microwaves, Chromosomes, Human, Pair 15, Chromosomes, Human, Pair 17, Leukemia, Promyelocytic, Acute genetics, Oncogene Proteins, Fusion genetics, Translocation, Genetic
- Abstract
Acute promyelocytic leukaemia (APL) is a hematologic malignancy characterized by the rearrangement of the PML and RARα genes, mostly due to a reciprocal chromosomal translocation t(15;17)(q24;q21). A quick APL diagnosis is essential for starting a prompt suitable therapy. We describe a new rapid diagnostic laboratory approach to detect the PML-RARα rearrangement, which gives clear genetic results within 30 min of hybridization. It combines quick cell harvesting, fluorescence in situ hybridization performed with commercial DNA probe and microwave beams supplied by a domestic microwave oven. Copyright © 2015 John Wiley & Sons, Ltd., (Copyright © 2015 John Wiley & Sons, Ltd.)
- Published
- 2017
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