72 results on '"Jing Yuan"'
Search Results
2. The road to a world-unified approach to the management of patients with gastric intestinal metaplasia: a review of current guidelines
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Dinis-Ribeiro, Mario, Shah, Shailja, El-Serag, Hashem, Banks, Matthew, Uedo, Noriya, Tajiri, Hisao, Coelho, Luiz Gonzaga, Libanio, Diogo, Lahner, Edith, Rollan, Antonio, Fang, Jing-Yuan, Moreira, Leticia, Bornschein, Jan, Malfertheiner, Peter, Kuipers, Ernst J, and El-Omar, Emad M
- Abstract
ObjectiveDuring the last decade, the management of gastric intestinal metaplasia (GIM) has been addressed by several distinct international evidence-based guidelines. In this review, we aimed to synthesise these guidelines and provide clinicians with a global perspective of the current recommendations for managing patients with GIM, as well as highlight evidence gaps that need to be addressed with future research.DesignWe conducted a systematic review of the literature for guidelines and consensus statements published between January 2010 and February 2023 that address the diagnosis and management of GIM.ResultsFrom 426 manuscripts identified, 16 guidelines were assessed. There was consistency across guidelines regarding the purpose of endoscopic surveillance of GIM, which is to identify prevalent neoplastic lesions and stage gastric preneoplastic conditions. The guidelines also agreed that only patients with high-risk GIM phenotypes (eg, corpus-extended GIM, OLGIM stages III/IV, incomplete GIM subtype), persistent refractory Helicobacter pyloriinfection or first-degree family history of gastric cancer should undergo regular-interval endoscopic surveillance. In contrast, low-risk phenotypes, which comprise most patients with GIM, do not require surveillance. Not all guidelines are aligned on histological staging systems. If surveillance is indicated, most guidelines recommend a 3-year interval, but there is some variability. All guidelines recommend H. pylorieradication as the only non-endoscopic intervention for gastric cancer prevention, while some offer additional recommendations regarding lifestyle modifications. While most guidelines allude to the importance of high-quality endoscopy for endoscopic surveillance, few detail important metrics apart from stating that a systematic gastric biopsy protocol should be followed. Notably, most guidelines comment on the role of endoscopy for gastric cancer screening and detection of gastric precancerous conditions, but with high heterogeneity, limited guidance regarding implementation, and lack of robust evidence.ConclusionDespite heterogeneous populations and practices, international guidelines are generally aligned on the importance of GIM as a precancerous condition and the need for a risk-stratified approach to endoscopic surveillance, as well as H. pylorieradication when present. There is room for harmonisation of guidelines regarding (1) which populations merit index endoscopic screening for gastric cancer and GIM detection/staging; (2) objective metrics for high-quality endoscopy; (3) consensus on the need for histological staging and (4) non-endoscopic interventions for gastric cancer prevention apart from H. pylorieradication alone. Robust studies, ideally in the form of randomised trials, are needed to bridge the ample evidence gaps that exist.
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- 2024
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3. Disruption of CerS6-mediated sphingolipid metabolism by FTO deficiency aggravates ulcerative colitis
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Ma, Yanru, Zhang, Xinyu, Xuan, Baoqin, Li, Danjie, Yin, Nan, Ning, Lijun, Zhou, Yi-Lu, Yan, Yuqing, Tong, Tianying, Zhu, Xiaoqiang, Huang, Xiaowen, Hu, Muni, Wang, Zhenhua, Cui, Zhe, Li, Huabin, Wang, Jiqiu, Fang, Jing-Yuan, Liu, Ruixin, Chen, Haoyan, and Hong, Jie
- Abstract
Background and aimsDeregulation of RNA N6-methyladenosine (m6A) modification in intestinal epithelial cells (IECs) influences intestinal immune cells and leads to intestinal inflammation. We studied the function of fat mass-and obesity-associated protein (FTO), one of the m6A demethylases, in patients with ulcerative colitis (UC).MethodsWe analysed colon tissues of Ftoflox/flox; Villin-cre mice and their Ftoflox/floxlittermates with dextran sulfate sodium (DSS) using real-time PCR and 16s rRNA sequencing. RNA and methylated RNA immunoprecipitation sequencing were used to analyse immunocytes and IECs. Macrophages were treated with conditioned medium of FTO-knockdown MODE-K cells or sphingosine-1-phosphate (S1P) and analysed for gene expression. Liquid chromatograph mass spectrometry identified C16-ceramide.ResultsFTO downregulation was identified in our in-house cohort and external cohorts of UC patients. Dysbiosis of gut microbiota, increased infiltration of proinflammatory macrophages, and enhanced differentiation of Th17 cells were observed in Ftoflox/flox;Villin-cre mice under DSS treatment. FTO deficiency resulted in an increase in m6A modification and a decrease in mRNA stability of CerS6, the gene encoding ceramide synthetase, leading to the downregulation of CerS6 and the accumulation of S1P in IECs. Subsequentially, the secretion of S1P by IECs triggered proinflammatory macrophages to secrete serum amyloid A protein 1/3, ultimately inducing Th17 cell differentiation. In addition, through bioinformatic analysis and experimental validation, we identified UC patients with lower FTO expression might respond better to vedolizumab treatment.ConclusionsFTO downregulation promoted UC by decreasing CerS6 expression, leading to increased S1P accumulation in IECs and aggravating colitis via m6A-dependent mechanisms. Lower FTO expression in UC patients may enhance their response to vedolizumab treatment.
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- 2024
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4. Chinese Consensus Report on Family-Based Helicobacter pylori Infection Control and Management (2021 Edition)
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Wei-Hong Wang, Shiyao Chen, Jianzhong Zhang, Wei-Fen Xie, Xing-Zhou Xia, Yuan Yuan, Yong Xie, Bing-Yong Zhang, Xiaohua Hou, Chengwei Tang, Jian-Qiu Sheng, Bo Jiang, Zhen-Yu Zhang, Yin Zhu, Chun-Hui Lan, Ying Han, Yan Li, Song-Ze Ding, Yi-Qi Du, Yan-Qing Li, Kaichun Wu, Jing-Tong Wang, Gui-Bin Yang, Ye Chen, Gui-Ying Zhang, Yinglei Miao, Hong Lu, Yunsheng Yang, Changqing Yang, Jiaming Qian, Jing-Yuan Fang, Xiu-Li Zuo, Jingnan Li, Bin Lyu, Nong-Hua Lyu, Guo-Xin Zhang, Fu-Lian Hu, Zhirong Zeng, Jian-Ming Xu, Yongzhan Nie, Mingzhou Guo, Hua-Hong Wang, Minhu Chen, Heng-Jun Gao, Hai-Xing Jiang, Xue-Hong Wang, Jun-Ping Wang, Li-Ya Zhou, Wei-Chang Chen, Hong Cheng, Jiang-Bin Wang, Zhao-Shen Li, Youming Li, Peng-Yuan Zheng, Jie Liu, Youyong Lu, and Fen Wang
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Mainland China ,Adult ,medicine.medical_specialty ,China ,13C-urea breath test ,Consensus ,Adolescent ,Delphi Technique ,Delphi method ,Guidelines ,mucosal infection ,Helicobacter Infections ,Young Adult ,medicine ,Infection control ,Humans ,Grading (education) ,Child ,Disease burden ,Aged ,Family Health ,Infection Control ,biology ,Helicobacter pylori ,Transmission (medicine) ,business.industry ,gastric cancer ,Gastroenterology ,Infant ,Middle Aged ,biology.organism_classification ,Infectious disease (medical specialty) ,Family medicine ,Child, Preschool ,helicobacter pylori - gastritis ,business - Abstract
Objective Helicobacter pyloriinfection is mostly a family-based infectious disease. To facilitate its prevention and management, a national consensus meeting was held to review current evidence and propose strategies for population-wide and family-basedH. pyloriinfection control and management to reduce the related disease burden. Methods Fifty-seven experts from 41 major universities and institutions in 20 provinces/regions of mainland China were invited to review evidence and modify statements using Delphi process and grading of recommendations assessment, development and evaluation system. The consensus level was defined as ≥80% for agreement on the proposed statements. Results Experts discussed and modified the original 23 statements on family-basedH. pyloriinfection transmission, control and management, and reached consensus on 16 statements. The final report consists of three parts: (1)H. pyloriinfection and transmission among family members, (2) prevention and management ofH. pyloriinfection in children and elderly people within households, and (3) strategies for prevention and management ofH. pyloriinfection for family members. In addition to the ‘test-and-treat’ and ‘screen-and-treat’ strategies, this consensus also introduced a novel third ‘family-basedH. pyloriinfection control and management’ strategy to prevent its intrafamilial transmission and development of related diseases. Conclusion H. pyloriis transmissible from person to person, and among family members. A family-basedH. pyloriprevention and eradication strategy would be a suitable approach to prevent its intra-familial transmission and related diseases. The notion and practice would be beneficial not only for Chinese residents but also valuable as a reference for other highly infected areas.
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- 2021
5. F. nucleatum targets lncRNA ENO1-IT1 to promote glycolysis and oncogenesis in colorectal cancer
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Xinyu Zhang, Ming Zhong, Jie Hong, Tian-Tian Sun, Haoyan Chen, Yanshen Peng, Xiong Ma, Jing-Yuan Fang, Cheng Wang, Yun Qian, Dan Ma, Tingting Yan, Shiyuan Lu, TaChung Yu, Jinxian Chen, Fangfang Guo, Yile Xie, Chaoqin Shen, Qiang Liu, Jianjun Liu, Ying-Xuan Chen, and Xiang Zhou
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0301 basic medicine ,Sp1 transcription factor ,biology ,Colorectal cancer ,Cell ,Gastroenterology ,Promoter ,Histone acetyltransferase ,medicine.disease_cause ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Histone ,medicine.anatomical_structure ,Transcription (biology) ,030220 oncology & carcinogenesis ,biology.protein ,medicine ,Cancer research ,Carcinogenesis - Abstract
ObjectiveMicrobiota disorder promotes chronic inflammation and carcinogenesis. High glycolysis is associated with poor prognosis in patients with colorectal cancer (CRC). However, the potential correlation between the gut microbiota and glucose metabolism is unknown in CRC.Design18F-FDG (18F-fluorodeoxyglucose) PET (positron emission tomography)/CT image scanning data and microbiota PCR analysis were performed to measure the correlation between metabolic alterations and microbiota disorder in 33 patients with CRC. Multiple colorectal cancer models, metabolic analysis and Seahorse assay were established to assess the role of long non-coding RNA (lncRNA) enolase1-intronic transcript 1 (ENO1-IT1) in Fusobacterium (F.) nucleatum-induced glucose metabolism and colorectal carcinogenesis. RNA immunoprecipitation and chromatin immunoprecipitation sequencing were conducted to identify potential targets of lncRNA ENO1-IT1.ResultsWe have found F. nucleatum abundance correlated with high glucose metabolism in patients with CRC. Furthermore, F. nucleatum supported carcinogenesis via increasing CRC cell glucose metabolism. Mechanistically, F. nucleatum activated lncRNA ENO1-IT1 transcription via upregulating the binding efficiency of transcription factor SP1 to the promoter region of lncRNA ENO1-IT1. Elevated ENO1-IT behaved as a guider modular for KAT7 histone acetyltransferase, specifying the histone modification pattern on its target genes, including ENO1, and consequently altering CRC biological function.ConclusionF. nucleatum and glucose metabolism are mechanistically, biologically and clinically connected to CRC. Targeting ENO1 pathway may be meaningful in treating patients with CRC with elevated F. nucleatum.
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- 2020
6. A novel faecal Lachnoclostridium marker for the non-invasive diagnosis of colorectal adenoma and cancer
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Tong Li, Ying-Xuan Chen, Siew C. Ng, Jing-Yuan Fang, Francis K.L. Chan, Joseph J.Y. Sung, Jessie Qiaoyi Liang, Eagle S. H. Chu, Geicho Nakatsu, Sunny H. Wong, Chun Ho Szeto, Jun Yu, and Tung On Yau
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Clostridium hathewayi ,medicine.medical_specialty ,biology ,Adenoma ,business.industry ,Colorectal cancer ,Gastroenterology ,Cancer ,Colorectal adenoma ,medicine.disease ,biology.organism_classification ,Real-time polymerase chain reaction ,Metagenomics ,Internal medicine ,medicine ,Fusobacterium nucleatum ,business - Abstract
ObjectiveThere is a need for early detection of colorectal cancer (CRC) at precancerous-stage adenoma. Here, we identified novel faecal bacterial markers for diagnosing adenoma.DesignThis study included 1012 subjects (274 CRC, 353 adenoma and 385 controls) from two independent Asian groups. Candidate markers were identified by metagenomics and validated by targeted quantitative PCR.ResultsMetagenomic analysis identified ‘m3’ from a Lachnoclostridium sp., Fusobacterium nucleatum (Fn) and Clostridium hathewayi (Ch) to be significantly enriched in adenoma. Faecal m3 and Fn were significantly increased from normal to adenoma to CRC (pm3 may perform better than Fn in distinguishing adenoma from controls (areas under the receiver operating characteristic curve (AUROCs) m3=0.675 vs Fn=0.620, p=0.09), while Fn performed better in diagnosing CRC (AUROCs Fn=0.862 vs m3=0.741, pm3 and Fn showed sensitivities of 48.3% and 33.8% for adenoma, and 62.1% and 77.8% for CRC, respectively. In a subgroup tested with faecal immunochemical test (FIT; n=642), m3 performed better than FIT in detecting adenoma (sensitivities for non-advanced and advanced adenomas of 44.2% and 50.8% by m3 (specificity=79.6%) vs 0% and 16.1% by FIT (specificity=98.5%)). Combining with FIT improved sensitivity of m3 for advanced adenoma to 56.8%. The combination of m3 with Fn, Ch, Bacteroides clarus and FIT performed best for diagnosing CRC (specificity=81.2% and sensitivity=93.8%).ConclusionThis study identifies a novel bacterial marker m3 for the non-invasive diagnosis of colorectal adenoma.
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- 2019
7. Chinese Consensus Report on Family-BasedHelicobacter pyloriInfection Control and Management (2021 Edition)
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Ding, Song-Ze, primary, Du, Yi-Qi, additional, Lu, Hong, additional, Wang, Wei-Hong, additional, Cheng, Hong, additional, Chen, Shi-Yao, additional, Chen, Min-Hu, additional, Chen, Wei-Chang, additional, Chen, Ye, additional, Fang, Jing-Yuan, additional, Gao, Heng-Jun, additional, Guo, Ming-Zhou, additional, Han, Ying, additional, Hou, Xiao-Hua, additional, Hu, Fu-Lian, additional, Jiang, Bo, additional, Jiang, Hai-Xing, additional, Lan, Chun-Hui, additional, Li, Jing-Nan, additional, Li, Yan, additional, Li, Yan-Qing, additional, Liu, Jie, additional, LI, You-Ming, additional, Lyu, Bin, additional, Lu, You-Yong, additional, Miao, Ying-Lei, additional, Nie, Yong-Zhan, additional, Qian, Jia-Ming, additional, Sheng, Jian-Qiu, additional, Tang, Cheng-Wei, additional, Wang, Fen, additional, Wang, Hua-Hong, additional, Wang, Jiang-Bin, additional, Wang, Jing-Tong, additional, Wang, Jun-Ping, additional, Wang, Xue-Hong, additional, Wu, Kai-Chun, additional, Xia, Xing-Zhou, additional, Xie, Wei-Fen, additional, Xie, Yong, additional, Xu, Jian-Ming, additional, Yang, Chang-Qing, additional, Yang, Gui-Bin, additional, Yuan, Yuan, additional, Zeng, Zhi-Rong, additional, Zhang, Bing-Yong, additional, Zhang, Gui-Ying, additional, Zhang, Guo-Xin, additional, Zhang, Jian-Zhong, additional, Zhang, Zhen-Yu, additional, Zheng, Peng-Yuan, additional, Zhu, Yin, additional, Zuo, Xiu-Li, additional, Zhou, Li-Ya, additional, Lyu, Nong-Hua, additional, Yang, Yun-Sheng, additional, and Li, Zhao-Shen, additional
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- 2021
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8. Alterations of gut microbiome in autoimmune hepatitis
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Qixia Wang, Chunyan Sun, Bo Li, Xiong Ma, Yiran Wei, Qi Miao, Qin Cao, Jing-Yuan Fang, Zhuping Fan, Jun Zhang, Bingyuan Huang, You Li, Li Yan, Xiaoyu Chen, Yong Chen, M. E. Gershwin, Yanmei Li, Yikang Li, Ruqi Tang, Xiao Xiao, and Min Lian
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Adult ,Male ,Adolescent ,Autoimmune hepatitis ,Gut flora ,medicine.disease_cause ,Severity of Illness Index ,Autoimmunity ,Veillonella ,Disease activity ,Young Adult ,medicine ,Humans ,Aspartate Aminotransferases ,Aged ,Clostridiales ,biology ,business.industry ,Gastroenterology ,Middle Aged ,biology.organism_classification ,medicine.disease ,Gut microbiome ,Gastrointestinal Microbiome ,Hepatitis, Autoimmune ,Lactobacillus ,Cross-Sectional Studies ,Case-Control Studies ,Immunology ,Cohort ,16s rrna gene sequencing ,Dysbiosis ,Female ,Gut dysbiosis ,business - Abstract
ObjectiveThe significance of the liver-microbiome axis has been increasingly recognised as a major modulator of autoimmunity. The aim of this study was to take advantage of a large well-defined corticosteroids treatment-naïve group of patients with autoimmune hepatitis (AIH) to rigorously characterise gut dysbiosis compared with healthy controls.DesignWe performed a cross-sectional study of individuals with AIH (n=91) and matched healthy controls (n=98) by 16S rRNA gene sequencing. An independent cohort of 28 patients and 34 controls was analysed to validate the results. All the patients were collected before corticosteroids therapy.ResultsThe gut microbiome of steroid treatment-naïve AIH was characterised with lower alpha-diversity (Shannon and observed operational taxonomic units, both pVeillonella were associated with disease status. Of note, Veillonella dispar, the most strongly disease-associated taxa (p=8.85E–8), positively correlated with serum level of aspartate aminotransferase and liver inflammation. Furthermore, the combination of four patients with AIH-associated genera distinguished AIH from controls with an area under curves of approximately 0.8 in both exploration and validation cohorts. In addition, multiple predicted functional modules were altered in the AIH gut microbiome, including lipopolysaccharide biosynthesis as well as metabolism of amino acids that can be processed by bacteria to produce immunomodulatory metabolites.ConclusionOur study establishes compositional and functional alterations of gut microbiome in AIH and suggests the potential for using gut microbiota as non-invasive biomarkers to assess disease activity.
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- 2019
9. OCT1 is a determinant of synbindin-related ERK signalling with independent prognostic significance in gastric cancer
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Qian, Jin, Kong, Xuan, Deng, Niantao, Tan, Patrick, Chen, Haoyan, Wang, Jilin, Li, Zhaoli, Hu, Ye, Zou, Weiping, Xu, Jie, and Fang, Jing-Yuan
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- 2015
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10. Carbon dioxide insufflation improves the intubation depth and total enteroscopy rate in single-balloon enteroscopy: a randomised, controlled, double-blind trial
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Li, Xuan, Zhao, Yun-Jia, Dai, Jun, Li, Xiao-Bo, Xue, Han-Bin, Zhang, Yao, Xiong, Guang-Su, Ohtsuka, Kazuo, Gao, Yun-Jie, Liu, Qiang, Song, Yan, Fang, Jing-Yuan, and Ge, Zhi-Zheng
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- 2014
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11. Altered faecal microbiome and metabolome in IgG4-related sclerosing cholangitis and primary sclerosing cholangitis
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Liu, Qiaoyan, primary, Li, Bo, additional, Li, Yikang, additional, Wei, Yiran, additional, Huang, Bingyuan, additional, Liang, Jubo, additional, You, Zhengrui, additional, Li, You, additional, Qian, Qiwei, additional, Wang, Rui, additional, Zhang, Jun, additional, Chen, Ruiling, additional, Lyu, Zhuwan, additional, Chen, Yong, additional, Shi, Mingxia, additional, Xiao, Xiao, additional, Wang, Qixia, additional, Miao, Qi, additional, Fang, Jing-Yuan, additional, Gershwin, Merrill Eric, additional, Lian, Min, additional, Ma, Xiong, additional, and Tang, Ruqi, additional
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- 2021
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12. Altered faecal microbiome and metabolome in IgG4-related sclerosing cholangitis and primary sclerosing cholangitis
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Min Lian, Jubo Liang, Mingxia Shi, Yong Chen, Zhengrui You, Qiaoyan Liu, Ruiling Chen, Qi Miao, Zhuwan Lyu, Qixia Wang, Bo Li, Bingyuan Huang, Jing-Yuan Fang, Rui Wang, M. E. Gershwin, Yikang Li, Jun Zhang, You Li, Xiao Xiao, Yiran Wei, Ruqi Tang, Qiwei Qian, and Xiong Ma
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0301 basic medicine ,Cholangitis, Sclerosing ,Gut flora ,digestive system ,Primary sclerosing cholangitis ,03 medical and health sciences ,0302 clinical medicine ,Cholestasis ,RNA, Ribosomal, 16S ,parasitic diseases ,medicine ,Metabolome ,Humans ,Eubacterium ,Microbiome ,Autoimmune disease ,biology ,digestive, oral, and skin physiology ,Hepatobiliary disease ,Gastroenterology ,medicine.disease ,biology.organism_classification ,Gastrointestinal Microbiome ,030104 developmental biology ,Immunoglobulin G ,Immunology ,030211 gastroenterology & hepatology - Abstract
ObjectiveMultiple clinical similarities exist between IgG4-related sclerosing cholangitis (IgG4-SC) and primary sclerosing cholangitis (PSC), and while gut dysbiosis has been extensively studied in PSC, the role of the gut microbiota in IgG4-SC remains unknown. Herein, we aimed to evaluate alterations of the gut microbiome and metabolome in IgG4-SC and PSC.DesignWe performed 16S rRNA gene amplicon sequencing of faecal samples from 135 subjects with IgG4-SC (n=34), PSC (n=37) and healthy controls (n=64). A subset of the samples (31 IgG4-SC, 37 PSC and 45 controls) also underwent untargeted metabolomic profiling.ResultsCompared with controls, reduced alpha-diversity and shifted microbial community were observed in IgG4-SC and PSC. These changes were accompanied by differences in stool metabolomes. Importantly, despite some common variations in the microbiota composition and metabolic activity, integrative analyses identified distinct host–microbe associations in IgG4-SC and PSC. The disease-associated genera and metabolites tended to associate with the transaminases in IgG4-SC. Notable depletion ofBlautiaand elevated succinic acid may underlie hepatic inflammation in IgG4-SC. In comparison, potential links between the microbial or metabolic signatures and cholestatic parameters were detected in PSC. Particularly, concordant decrease ofEubacteriumand microbiota-derived metabolites, including secondary bile acids, implicated novel host–microbial metabolic pathways involving cholestasis of PSC. Interestingly, the predictive models based on metabolites were more effective in discriminating disease status than those based on microbes.ConclusionsOur data reveal that IgG4-SC and PSC possess divergent host–microbe interplays that may be involved in disease pathogenesis. These data emphasise the uniqueness of IgG4-SC.
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- 2020
13. Altered faecal microbiome and metabolome in IgG4-related sclerosing cholangitis and primary sclerosing cholangitis.
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Qiaoyan Liu, Bo Li, Yikang Li, Yiran Wei, Bingyuan Huang, Jubo Liang, Zhengrui You, You Li, Qiwei Qian, Rui Wang, Jun Zhang, Ruiling Chen, Zhuwan Lyu, Yong Chen, Mingxia Shi, Xiao Xiao, Qixia Wang, Qi Miao, Jing-Yuan Fang, and Gershwin, Merrill Eric
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CHOLANGITIS ,LIQUID chromatography-mass spectrometry - Published
- 2022
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14. Ginseng polysaccharides alter the gut microbiota and kynurenine/tryptophan ratio, potentiating the antitumour effect of antiprogrammed cell death 1/programmed cell death ligand 1 (anti-PD-1/PD-L1) immunotherapy.
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Jumin Huang, Di Liu, Yuwei Wang, Liang Liu, Jian Li, Jing Yuan, Zhihong Jiang, Zebo Jiang, Hsiao, W. L. Wendy, Haizhou Liu, Khan, Imran, Ying Xie, Jianlin Wu, Yajia Xie, Yizhong Zhang, Yu Fu, Junyi Liao, Wenjun Wang, Huanling Lai, and Axi Shi
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PEMBROLIZUMAB ,GUT microbiome ,CELL death ,MELANOMA ,KYNURENINE ,LIQUID chromatography-mass spectrometry ,TRYPTOPHAN - Published
- 2022
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15. Ginseng polysaccharides alter the gut microbiota and kynurenine/tryptophan ratio, potentiating the antitumour effect of antiprogrammed cell death 1/programmed cell death ligand 1 (anti-PD-1/PD-L1) immunotherapy
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Xiao-Jun Yao, Lin Wang, Wen-Jun Wang, Imran Khan, Jian-Lin Wu, Ya-Jia Xie, Yabing Cao, W.L. Wendy Hsiao, A-Xi Shi, Ze-Bo Jiang, Yuwei Wang, Qibiao Wu, Lian-Xiang Luo, Zhi-Hong Jiang, Jing-Guang Lu, Yu Fu, Hong Wei, Jun Cai, Elaine Lai-Han Leung, Di Liu, Ying Xie, Ju-Min Huang, Zhongqiu Liu, Haizhou Liu, Huan-Ling Lai, Jian Li, Ming-Rong Yang, Pei-Yu Yan, Liang Liu, Yi-Zhong Zhang, Xing-Xing Fan, Jing Yuan, Run-Ze Li, and Junyi Liao
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0301 basic medicine ,Lung Neoplasms ,Combination therapy ,medicine.drug_class ,medicine.medical_treatment ,Panax ,Apoptosis ,Gut flora ,Pharmacology ,Monoclonal antibody ,Ligands ,B7-H1 Antigen ,03 medical and health sciences ,chemistry.chemical_compound ,Ginseng ,Mice ,0302 clinical medicine ,Polysaccharides ,PD-L1 ,Carcinoma, Non-Small-Cell Lung ,medicine ,Animals ,Humans ,Immunologic Factors ,Kynurenine ,biology ,Cell Death ,business.industry ,Prebiotic ,Gastroenterology ,Tryptophan ,Antibodies, Monoclonal ,Immunotherapy ,biology.organism_classification ,Gastrointestinal Microbiome ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,business - Abstract
ObjectiveProgrammed death 1 and its ligand 1 (PD-1/PD-L1) immunotherapy is promising for late-stage lung cancer treatment, however, the response rate needs to be improved. Gut microbiota plays a crucial role in immunotherapy sensitisation and Panax ginseng has been shown to possess immunomodulatory potential. In this study, we aimed to investigate whether the combination treatment of ginseng polysaccharides (GPs) and αPD-1 monoclonal antibody (mAb) could sensitise the response by modulating gut microbiota.DesignSyngeneic mouse models were administered GPs and αPD-1 mAb, the sensitising antitumour effects of the combination therapy on gut microbiota were assessed by faecal microbiota transplantation (FMT) and 16S PacBio single-molecule real-time (SMRT) sequencing. To assess the immune-related metabolites, metabolomics analysis of the plasma samples was performed.ResultsWe found GPs increased the antitumour response to αPD-1 mAb by increasing the microbial metabolites valeric acid and decreasing L-kynurenine, as well as the ratio of Kyn/Trp, which contributed to the suppression of regulatory T cells and induction of Teff cells after combination treatment. Besides, the microbial analysis indicated that the abundance of Parabacteroides distasonis and Bacteroides vulgatus was higher in responders to anti-PD-1 blockade than non-responders in the clinic. Furthermore, the combination therapy sensitised the response to PD-1 inhibitor in the mice receiving microbes by FMT from six non-responders by reshaping the gut microbiota from non-responders towards that of responders.ConclusionOur results demonstrate that GPs combined with αPD-1 mAb may be a new strategy to sensitise non-small cell lung cancer patients to anti-PD-1 immunotherapy. The gut microbiota can be used as a novel biomarker to predict the response to anti-PD-1 immunotherapy.
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- 2020
16. F. nucleatum targets lncRNA ENO1-IT1 to promote glycolysis and oncogenesis in colorectal cancer
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Hong, Jie, primary, Guo, Fangfang, additional, Lu, Shi-Yuan, additional, Shen, Chaoqin, additional, Ma, Dan, additional, Zhang, Xinyu, additional, Xie, Yile, additional, Yan, Tingting, additional, Yu, TaChung, additional, Sun, Tiantian, additional, Qian, Yun, additional, Zhong, Ming, additional, Chen, Jinxian, additional, Peng, Yanshen, additional, Wang, Cheng, additional, Zhou, Xiang, additional, Liu, Jianjun, additional, Liu, Qiang, additional, Ma, Xiong, additional, Chen, Ying-Xuan, additional, Chen, Haoyan, additional, and Fang, Jing-Yuan, additional
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- 2020
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17. Chinese Consensus Report on Family-Based Helicobacter pylori Infection Control and Management (2021 Edition).
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Song-Ze Ding, Yi-Qi Du, Hong Lu, Wei-Hong Wang, Hong Cheng, Shi-Yao Chen, Min-Hu Chen, Wei-Chang Chen, Ye Chen, Jing-Yuan Fang, Heng-Jun Gao, Ming-Zhou Guo, Ying Han, Xiao-Hua Hou, Fu-Lian Hu, Bo Jiang, Hai-Xing Jiang, Chun-Hui Lan, Jing-Nan Li, and Yan Li
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HELICOBACTER pylori infections ,INFECTION control - Published
- 2022
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18. The complicated dialogue between Helicobacter pylori and p53
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Tao, Zhi-Hang, primary and Fang, Jing-Yuan, additional
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- 2020
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19. A novel faecal Lachnoclostridium marker for the non-invasive diagnosis of colorectal adenoma and cancer
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Liang, Jessie Qiaoyi, primary, Li, Tong, additional, Nakatsu, Geicho, additional, Chen, Ying-Xuan, additional, Yau, Tung On, additional, Chu, Eagle, additional, Wong, Sunny, additional, Szeto, Chun Ho, additional, Ng, Siew C, additional, Chan, Francis K L, additional, Fang, Jing-Yuan, additional, Sung, Joseph J Y, additional, and Yu, Jun, additional
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- 2019
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20. Gut microbial profile is altered in primary biliary cholangitis and partially restored after UDCA therapy
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Aftab A. Ansari, Xiong Ma, Dekai Qiu, Ruqi Tang, M. Eric Gershwin, Xiang Jiang, Yiran Wei, Qin Cao, Haiyan Zhang, Min Lian, Weihua Chen, Jun Zhang, Qixia Wang, Fan Yang, Maoying Wu, Haoyan Chen, Jing-Yuan Fang, Xiao Xiao, Yanmei Li, Zhuping Fan, and Qi Miao
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Adult ,Male ,0301 basic medicine ,Cholagogues and Choleretics ,medicine.medical_specialty ,Pathology ,Cholangitis ,Gut flora ,digestive system ,Gastroenterology ,Antibodies ,Feces ,Young Adult ,03 medical and health sciences ,Primary biliary cirrhosis ,Internal medicine ,medicine ,Humans ,Prospective Studies ,Microbiome ,Prospective cohort study ,Aged ,Bacteria ,biology ,Ursodeoxycholic Acid ,Middle Aged ,biology.organism_classification ,medicine.disease ,Enterobacteriaceae ,Ursodeoxycholic acid ,Gastrointestinal Microbiome ,Nuclear Pore Complex Proteins ,Cross-Sectional Studies ,030104 developmental biology ,Case-Control Studies ,Dysbiosis ,Female ,Biomarkers ,medicine.drug - Abstract
ObjectiveA close relationship between gut microbiota and some chronic liver disorders has recently been described. Herein, we systematically performed a comparative analysis of the gut microbiome in primary biliary cholangitis (PBC) and healthy controls.DesignWe first conducted a cross-sectional study of 60 ursodeoxycholic acid (UDCA) treatment-naïve patients with PBC and 80 matched healthy controls. Second, an independent cohort composed of 19 treatment-naïve patients and 34 controls was used to validate the results. Finally, a prospective study was performed in a subgroup of 37 patients with PBC who underwent analysis before and after 6 months of UDCA treatment. Faecal samples were collected, and microbiomes were analysed by 16S ribosomal RNA gene sequencing.ResultsA significant reduction of within-individual microbial diversity was noted in PBC (p=0.03). A signature defined by decreased abundance of four genera and increased abundance of eight genera strongly correlated with PBC (area under curve=0.86, 0.84 in exploration and validation data, respectively). Notably, the abundance of six PBC-associated genera was reversed after 6 months of UDCA treatment. In particular, Faecalibacterium, enriched in controls, was further decreased in gp210-positive than gp210-negative patients (p=0.002). Of interest was the finding that the increased capacity for the inferred pathway, bacterial invasion of epithelial cells in PBC, highly correlated with the abundance of bacteria belonging to Enterobacteriaceae.ConclusionsThis study presents a comprehensive landscape of gut microbiota in PBC. Dysbiosis was found in the gut microbiome in PBC and partially relieved by UDCA. Our study suggests that gut microbiota is a potential therapeutic target and diagnostic biomarker for PBC.
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- 2017
21. The complicated dialogue between Helicobacter pylori and p53
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Jing-Yuan Fang and Zhi-Hang Tao
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biology ,business.industry ,Gastroenterology ,Cancer research ,USF1 ,biology.protein ,Medicine ,Helicobacter pylori ,business ,Gastric carcinogenesis ,biology.organism_classification ,Upstream Stimulatory Factor - Abstract
We recently read the interesting paper published in GUT elucidating the role of upstream stimulatory factor 1 (USF1) in Helicobacter pylori (Hp )-induced p53 degradation.1 The work greatly broadens our knowledge of the interplay between Hp and p53 during gastric carcinogenesis. Here we propose some additional exploration, if incorporated in study design, can further deepen our understanding. The significance of USF1 in gastric carcinogenesis should be acknowledged as the study showed that loss of USF1, on its own, exacerbated the severity of …
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- 2020
22. A novel faecal
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Jessie Qiaoyi, Liang, Tong, Li, Geicho, Nakatsu, Ying-Xuan, Chen, Tung On, Yau, Eagle, Chu, Sunny, Wong, Chun Ho, Szeto, Siew C, Ng, Francis K L, Chan, Jing-Yuan, Fang, Joseph J Y, Sung, and Jun, Yu
- Subjects
Adenoma ,Male ,Clostridiales ,colorectal adenomas ,Middle Aged ,Polymerase Chain Reaction ,Sensitivity and Specificity ,colorectal cancer screening ,digestive system diseases ,colonic bacteria ,stomatognathic diseases ,Feces ,Case-Control Studies ,Biomarkers, Tumor ,Humans ,Female ,Metagenomics ,Gut Microbiota ,Colorectal Neoplasms - Abstract
Objective There is a need for early detection of colorectal cancer (CRC) at precancerous-stage adenoma. Here, we identified novel faecal bacterial markers for diagnosing adenoma. Design This study included 1012 subjects (274 CRC, 353 adenoma and 385 controls) from two independent Asian groups. Candidate markers were identified by metagenomics and validated by targeted quantitative PCR. Results Metagenomic analysis identified ‘m3’ from a Lachnoclostridium sp., Fusobacterium nucleatum (Fn) and Clostridium hathewayi (Ch) to be significantly enriched in adenoma. Faecal m3 and Fn were significantly increased from normal to adenoma to CRC (p
- Published
- 2019
23. Altered faecal microbiome and metabolome in IgG4-related sclerosing cholangitis and primary sclerosing cholangitis
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Liu, Qiaoyan, Li, Bo, Li, Yikang, Wei, Yiran, Huang, Bingyuan, Liang, Jubo, You, Zhengrui, Li, You, Qian, Qiwei, Wang, Rui, Zhang, Jun, Chen, Ruiling, Lyu, Zhuwan, Chen, Yong, Shi, Mingxia, Xiao, Xiao, Wang, Qixia, Miao, Qi, Fang, Jing-Yuan, Gershwin, Merrill Eric, Lian, Min, Ma, Xiong, and Tang, Ruqi
- Abstract
ObjectiveMultiple clinical similarities exist between IgG4-related sclerosing cholangitis (IgG4-SC) and primary sclerosing cholangitis (PSC), and while gut dysbiosis has been extensively studied in PSC, the role of the gut microbiota in IgG4-SC remains unknown. Herein, we aimed to evaluate alterations of the gut microbiome and metabolome in IgG4-SC and PSC.DesignWe performed 16S rRNA gene amplicon sequencing of faecal samples from 135 subjects with IgG4-SC (n=34), PSC (n=37) and healthy controls (n=64). A subset of the samples (31 IgG4-SC, 37 PSC and 45 controls) also underwent untargeted metabolomic profiling.ResultsCompared with controls, reduced alpha-diversity and shifted microbial community were observed in IgG4-SC and PSC. These changes were accompanied by differences in stool metabolomes. Importantly, despite some common variations in the microbiota composition and metabolic activity, integrative analyses identified distinct host–microbe associations in IgG4-SC and PSC. The disease-associated genera and metabolites tended to associate with the transaminases in IgG4-SC. Notable depletion of Blautiaand elevated succinic acid may underlie hepatic inflammation in IgG4-SC. In comparison, potential links between the microbial or metabolic signatures and cholestatic parameters were detected in PSC. Particularly, concordant decrease of Eubacteriumand microbiota-derived metabolites, including secondary bile acids, implicated novel host–microbial metabolic pathways involving cholestasis of PSC. Interestingly, the predictive models based on metabolites were more effective in discriminating disease status than those based on microbes.ConclusionsOur data reveal that IgG4-SC and PSC possess divergent host–microbe interplays that may be involved in disease pathogenesis. These data emphasise the uniqueness of IgG4-SC.
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- 2022
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24. Alterations of gut microbiome in autoimmune hepatitis
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Wei, Yiran, primary, Li, Yanmei, additional, Yan, Li, additional, Sun, Chunyan, additional, Miao, Qi, additional, Wang, Qixia, additional, Xiao, Xiao, additional, Lian, Min, additional, Li, Bo, additional, Chen, Yong, additional, Zhang, Jun, additional, Li, You, additional, Huang, Bingyuan, additional, Li, Yikang, additional, Cao, Qin, additional, Fan, Zhuping, additional, Chen, Xiaoyu, additional, Fang, Jing-Yuan, additional, Gershwin, Merrill Eric, additional, Tang, Ruqi, additional, and Ma, Xiong, additional
- Published
- 2019
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25. OCT1 is a determinant of synbindin-related ERK signalling with independent prognostic significance in gastric cancer
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Ye Hu, Patrick Tan, Xuan Kong, Haoyan Chen, Ji-Lin Wang, Weiping Zou, Zhaoli Li, Jing-Yuan Fang, Jie Xu, Jin Qian, and Niantao Deng
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MAPK/ERK pathway ,Pathology ,medicine.medical_specialty ,MAP Kinase Signaling System ,Vesicular Transport Proteins ,Nerve Tissue Proteins ,Cell Migration ,Biology ,Mice ,Downregulation and upregulation ,ELK1 ,Stomach Neoplasms ,Cancer Genetics ,medicine ,Animals ,Extracellular Signal-Regulated MAP Kinases ,Protein kinase A ,Octamer transcription factor ,Cell Proliferation ,Kinase ,Stomach ,Organic Cation Transporter 1 ,Gastroenterology ,Cancer ,Prognosis ,medicine.disease ,Gastric Cancer ,Cancer research ,Signal transduction ,Signal Transduction - Abstract
Objective Octamer transcription factor 1 (OCT1) was found to be expressed in intestinal metaplasia and gastric cancer (GC), but the exact roles of OCT1 in GC remain unclear. The objective of this study was to determine the functional and prognostic implications of OCT1 in GC. Design Expression of OCT1 was examined in paired normal and cancerous gastric tissues and the prognostic significance of OCT1 was analysed by univariate and multivariate survival analyses. The functions of OCT1 on synbindin expression and extracellular signal-regulated kinase (ERK) phosphorylation were studied in vitro and in xenograft mouse models. Results The OCT1 gene is recurrently amplified and upregulated in GC. OCT1 overexpression and amplification are associated with poor survival in patients with GC and the prognostic significance was confirmed by independent patient cohorts. Combining OCT1 overexpression with American Joint Committee on Cancer staging improved the prediction of survival in patients with GC. High expression of OCT1 associates with activation of the ERK mitogen-activated protein kinase signalling pathway in GC tissues. OCT1 functions by transactivating synbindin, which binds to ERK DEF domain and facilitates ERK phosphorylation by MEK. OCT1-synbindin signalling results in the activation of ERK substrates ELK1 and RSK, leading to increased cell proliferation and invasion. Immunofluorescent study of human GC tissue samples revealed strong association between OCT1 protein level and synbindin expression/ERK phosphorylation. Upregulation of OCT1 in mouse xenograft models induced synbindin expression and ERK activation, leading to accelerated tumour growth in vivo. Conclusions OCT1 is a driver of synbindin-mediated ERK signalling and a promising marker for the prognosis and molecular subtyping of GC.
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- 2014
26. Several aspects in study design need further consideration
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Jing-Yuan Fang and Cheng-Bei Zhou
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medicine.medical_specialty ,biology ,Atrophic gastritis ,business.industry ,Gastroenterology ,Cancer ,Helicobacter pylori ,Helicobacter Infections ,medicine.disease ,biology.organism_classification ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,medicine ,030211 gastroenterology & hepatology ,Cancer development ,business ,Intensive care medicine - Abstract
We read with interest the work by Cheung et al 1 describing that patients with prior Helicobacter pylori eradication therapy receiving long-term proton pump inhibitors (PPIs) therapy are still at enhanced risk of gastric cancer development, particularly for non-cardia cancer. As the current consensus report stated, long-term treatment with PPIs in H. pylori -positive patients accelerates the process of loss of specialised glands, leading to atrophic gastritis. Hence, it is recommended that eradication of H. pylori …
- Published
- 2017
27. A novel faecal Lachnoclostridium marker for the non-invasive diagnosis of colorectal adenoma and cancer.
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Qiaoyi Liang, Jessie, Tong Li, Geicho Nakatsu, Ying-Xuan Chen, Tung On Yau, Chu, Eagle, Sunny Wong, Szeto, Chun Ho, Ng, Siew C., Chan, Francis K. L., Jing-Yuan Fang, Sung, Joseph J. Y., and Jun Yu
- Subjects
CLOSTRIDIA ,ADENOMATOUS polyps ,COLON cancer - Published
- 2020
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28. Alterations of gut microbiome in autoimmune hepatitis.
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Yiran Wei, Yanmei Li, Li Yan, Chunyan Sun, Qi Miao, Qixia Wang, Xiao Xiao, Min Lian, Bo Li, Yong Chen, Jun Zhang, You Li, Bingyuan Huang, Yikang Li, Qin Cao, Zhuping Fan, Xiaoyu Chen, Jing-Yuan Fang, Gershwin, Merrill Eric, and Ruqi Tang
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GUT microbiome ,CHRONIC active hepatitis ,INFLAMMATORY bowel diseases - Published
- 2020
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29. Multiple balloon-like lesions in the small intestine of an adult with chronic diarrhoea
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Zhou, Cheng-Bei, primary, Lu, Hong, additional, Chen, Ying-Xuan, additional, and Fang, Jing-Yuan, additional
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- 2018
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30. Several aspects in study design need further consideration
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Zhou, Cheng-Bei, primary and Fang, Jing-Yuan, additional
- Published
- 2017
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31. F. nucleatumtargets lncRNA ENO1-IT1 to promote glycolysis and oncogenesis in colorectal cancer
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Hong, Jie, Guo, Fangfang, Lu, Shi-Yuan, Shen, Chaoqin, Ma, Dan, Zhang, Xinyu, Xie, Yile, Yan, Tingting, Yu, TaChung, Sun, Tiantian, Qian, Yun, Zhong, Ming, Chen, Jinxian, Peng, Yanshen, Wang, Cheng, Zhou, Xiang, Liu, Jianjun, Liu, Qiang, Ma, Xiong, Chen, Ying-Xuan, Chen, Haoyan, and Fang, Jing-Yuan
- Abstract
ObjectiveMicrobiota disorder promotes chronic inflammation and carcinogenesis. High glycolysis is associated with poor prognosis in patients with colorectal cancer (CRC). However, the potential correlation between the gut microbiota and glucose metabolism is unknown in CRC.Design18F-FDG (18F-fluorodeoxyglucose) PET (positron emission tomography)/CT image scanning data and microbiota PCR analysis were performed to measure the correlation between metabolic alterations and microbiota disorder in 33 patients with CRC. Multiple colorectal cancer models, metabolic analysis and Seahorse assay were established to assess the role of long non-coding RNA (lncRNA) enolase1-intronic transcript 1 (ENO1-IT1) in Fusobacterium (F.) nucleatum-induced glucose metabolism and colorectal carcinogenesis. RNA immunoprecipitation and chromatin immunoprecipitation sequencing were conducted to identify potential targets of lncRNA ENO1-IT1.ResultsWe have found F. nucleatumabundance correlated with high glucose metabolism in patients with CRC. Furthermore, F. nucleatumsupported carcinogenesis via increasing CRC cell glucose metabolism. Mechanistically, F. nucleatumactivated lncRNA ENO1-IT1 transcription via upregulating the binding efficiency of transcription factor SP1 to the promoter region of lncRNA ENO1-IT1. Elevated ENO1-IT behaved as a guider modular for KAT7 histone acetyltransferase, specifying the histone modification pattern on its target genes, including ENO1, and consequently altering CRC biological function.ConclusionF. nucleatumand glucose metabolism are mechanistically, biologically and clinically connected to CRC. Targeting ENO1 pathway may be meaningful in treating patients with CRC with elevated F. nucleatum.
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- 2021
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32. Multiple balloon-like lesions in the small intestine of an adult with chronic diarrhoea
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Jing-Yuan Fang, Cheng-Bei Zhou, Ying-Xuan Chen, and Hong Lu
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Adult ,Diarrhea ,0301 basic medicine ,Abdominal pain ,medicine.medical_specialty ,medicine.drug_class ,Biopsy ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,Bloating ,Internal medicine ,Intestine, Small ,Antidiarrhoeal ,Palpitations ,Humans ,Medicine ,Intestinal Mucosa ,Family history ,biology ,business.industry ,Enteritis ,Small intestine ,Common Variable Immunodeficiency ,030104 developmental biology ,medicine.anatomical_structure ,Chronic Disease ,biology.protein ,Defecation ,Female ,030211 gastroenterology & hepatology ,Antibody ,medicine.symptom ,business - Abstract
Clinical presentationA 28-year-old woman presented with a 3-year history of chronic watery diarrhoea along with abdominal pain and bloating, which could mostly be alleviated after defecation. Her symptom of diarrhoea, at least three times a day, could be relieved by neither probiotics nor antidiarrhoeal agents. She had also lost 5 kg in the last month. She denied family history, poor vaccine responses or significant infections in early childhood except for an allergy history to intravenous immunoglobulin (Ig) with immediate dyspnoea, palpitations and hypotension. Laboratory investigations suggested that the stool specimens were negative for viruses, parasites or bacteria. Laboratory evaluation revealed a low serum globulin level, 14.5 (reference range, 20–30 g/L); serum Ig levels were significantly abnormal: IgA 9/L (3.69–9.16×109/L); C-reactive protein (CRP) 20.5 (normal Figure 1Endoscopic images show swelling mucosa, dense nodular lesions in duodenum (A), upper jejunum (B), upper ileum (C) and terminal ileum (D).QuestionWhat is the most likely diagnosis?
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- 2018
33. A tribute to Professor Shu Dong Xiao (1931–2016)
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Fang, Jing Yuan, primary
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- 2017
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34. Gut microbial profile is altered in primary biliary cholangitis and partially restored after UDCA therapy
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Tang, Ruqi, primary, Wei, Yiran, additional, Li, Yanmei, additional, Chen, Weihua, additional, Chen, Haoyan, additional, Wang, Qixia, additional, Yang, Fan, additional, Miao, Qi, additional, Xiao, Xiao, additional, Zhang, Haiyan, additional, Lian, Min, additional, Jiang, Xiang, additional, Zhang, Jun, additional, Cao, Qin, additional, Fan, Zhuping, additional, Wu, Maoying, additional, Qiu, Dekai, additional, Fang, Jing-Yuan, additional, Ansari, Aftab, additional, Gershwin, M Eric, additional, and Ma, Xiong, additional
- Published
- 2017
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35. Alterations of gut microbiome in autoimmune hepatitis
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Wei, Yiran, Li, Yanmei, Yan, Li, Sun, Chunyan, Miao, Qi, Wang, Qixia, Xiao, Xiao, Lian, Min, Li, Bo, Chen, Yong, Zhang, Jun, Li, You, Huang, Bingyuan, Li, Yikang, Cao, Qin, Fan, Zhuping, Chen, Xiaoyu, Fang, Jing-Yuan, Gershwin, Merrill Eric, Tang, Ruqi, and Ma, Xiong
- Abstract
ObjectiveThe significance of the liver-microbiome axis has been increasingly recognised as a major modulator of autoimmunity. The aim of this study was to take advantage of a large well-defined corticosteroids treatment-nai¨ve group of patients with autoimmune hepatitis (AIH) to rigorously characterise gut dysbiosis compared with healthy controls.DesignWe performed a cross-sectional study of individuals with AIH (n=91) and matched healthy controls (n=98) by 16S rRNA gene sequencing. An independent cohort of 28 patients and 34 controls was analysed to validate the results. All the patients were collected before corticosteroids therapy.ResultsThe gut microbiome of steroid treatment-nai¨ve AIH was characterised with lower alpha-diversity (Shannon and observed operational taxonomic units, both p<0.01) and distinct overall microbial composition compared with healthy controls (p=0.002). Depletion of obligate anaerobes and expansion of potential pathobionts including Veillonellawere associated with disease status. Of note, Veillonella dispar, the most strongly disease-associated taxa (p=8.85E–8), positively correlated with serum level of aspartate aminotransferase and liver inflammation. Furthermore, the combination of four patients with AIH-associated genera distinguished AIH from controls with an area under curves of approximately 0.8 in both exploration and validation cohorts. In addition, multiple predicted functional modules were altered in the AIH gut microbiome, including lipopolysaccharide biosynthesis as well as metabolism of amino acids that can be processed by bacteria to produce immunomodulatory metabolites.ConclusionOur study establishes compositional and functional alterations of gut microbiome in AIH and suggests the potential for using gut microbiota as non-invasive biomarkers to assess disease activity.
- Published
- 2020
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36. A novel faecal Lachnoclostridiummarker for the non-invasive diagnosis of colorectal adenoma and cancer
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Liang, Jessie Qiaoyi, Li, Tong, Nakatsu, Geicho, Chen, Ying-Xuan, Yau, Tung On, Chu, Eagle, Wong, Sunny, Szeto, Chun Ho, Ng, Siew C, Chan, Francis K L, Fang, Jing-Yuan, Sung, Joseph J Y, and Yu, Jun
- Abstract
ObjectiveThere is a need for early detection of colorectal cancer (CRC) at precancerous-stage adenoma. Here, we identified novel faecal bacterial markers for diagnosing adenoma.DesignThis study included 1012 subjects (274 CRC, 353 adenoma and 385 controls) from two independent Asian groups. Candidate markers were identified by metagenomics and validated by targeted quantitative PCR.ResultsMetagenomic analysis identified ‘m3’ from a Lachnoclostridiumsp., Fusobacterium nucleatum(Fn) and Clostridium hathewayi(Ch) to be significantly enriched in adenoma. Faecal m3and Fnwere significantly increased from normal to adenoma to CRC (p<0.0001, linear trend by one-way ANOVA) in group I (n=698), which was further confirmed in group II (n=313; p<0.0001). Faecal m3may perform better than Fnin distinguishing adenoma from controls (areas under the receiver operating characteristic curve (AUROCs) m3=0.675 vs Fn=0.620, p=0.09), while Fnperformed better in diagnosing CRC (AUROCs Fn=0.862 vs m3=0.741, p<0.0001). At 78.5% specificity, m3and Fnshowed sensitivities of 48.3% and 33.8% for adenoma, and 62.1% and 77.8% for CRC, respectively. In a subgroup tested with faecal immunochemical test (FIT; n=642), m3performed better than FIT in detecting adenoma (sensitivities for non-advanced and advanced adenomas of 44.2% and 50.8% by m3(specificity=79.6%) vs 0% and 16.1% by FIT (specificity=98.5%)). Combining with FIT improved sensitivity of m3for advanced adenoma to 56.8%. The combination of m3with Fn, Ch, Bacteroides clarusand FIT performed best for diagnosing CRC (specificity=81.2% and sensitivity=93.8%).ConclusionThis study identifies a novel bacterial marker m3for the non-invasive diagnosis of colorectal adenoma.
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- 2020
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37. A tribute to Professor Shu Dong Xiao (1931–2016)
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Jing Yuan Fang
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Gerontology ,China ,medicine.medical_specialty ,business.industry ,media_common.quotation_subject ,education ,Gastroenterology ,Tribute ,History, 20th Century ,History, 21st Century ,humanities ,Clinical study ,Affection ,Family medicine ,medicine ,business ,health care economics and organizations ,media_common - Abstract
Great sadness and deep affection marked the recent passing of Professor and Dr Shu Dong Xiao, a renowned and respected gastroenterologist, the emeritus director of Shanghai Institute of Digestive Disease and the emeritus professor of Renji Hospital, School of Medicine, Shanghai Jiao Tong University, who had dedicated his whole life to the academic and clinical study of digestive diseases. Professor Xiao died from cardiopulmonary failure on 22 July 2016 at the age of 85. Professor Xiao was born in 1931 in Huangpi city, Hubei Province, China. He graduated from the Shanghai Second Medical College (currently known as Shanghai Jiaotong University School of Medicine) in 1955, majoring in medicine. Because of his excellent performance he remained working as a …
- Published
- 2017
38. Carbon dioxide insufflation improves the intubation depth and total enteroscopy rate in single-balloon enteroscopy: a randomised, controlled, double-blind trial
- Author
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Han-Bin Xue, Jing-Yuan Fang, Xuan Li, Yun-jia Zhao, Jun Dai, Kazuo Ohtsuka, Yun-Jie Gao, Guang-Su Xiong, Qiang Liu, Zhi-Zheng Ge, Yao Zhang, Xiao-Bo Li, and Yan Song
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Insufflation ,Enteroscopy ,Adult ,Male ,medicine.medical_specialty ,China ,Adolescent ,medicine.medical_treatment ,Endoscopy, Gastrointestinal ,law.invention ,Double blind ,Young Adult ,Randomized controlled trial ,Double-Blind Method ,law ,medicine ,Intubation ,Humans ,Intubation, Gastrointestinal ,Carbon dioxide insufflation ,Aged ,business.industry ,Air ,Gastroenterology ,Single-Balloon Enteroscopy ,Carbon Dioxide ,Middle Aged ,Surgery ,Intention to Treat Analysis ,Anesthesia ,Female ,Air insufflation ,business - Abstract
The total enteroscopy rate of single-balloon enteroscopy (SBE) using air insufflation is not satisfactory, and whether carbon dioxide (CO2) insufflation increases the total enteroscopy rate of SBE is unknown. This randomised controlled trial aimed to determine whether CO2 insufflation facilitates the intubation depth and total enteroscopy rate of SBE.A total of 214 eligible patients referred for SBE were randomised to receive either air or CO2 insufflation, and included in the intention-to-test (ITT) analysis. In addition, 199 patients in whom enteroscopy was completed were included in the per-protocol (PP) analysis. Both the patients and endoscopists were blinded, and the intubation depth and total enteroscopy rate were defined as the primary outcomes.The CO2 group showed a superiority of intubation in the ITT analysis (oral route: 323.8±64.2 vs 238.3±68.6 cm; anal route: 261.6±74.2 vs 174.7±62.1 cm, both p0.001), and the total enteroscopy rate (34.9% vs 17.6%, p=0.006). Similar results were obtained in a PP analysis for both outcomes. In addition, in the PP analysis, the addition of circumference after the procedure was less in the CO2 group (0.8±0.6 vs 3.3±1.8 cm, p=0.005) for the oral route. No serious complications were reported. The overall percentage of procedures with significant pathological findings was 52.8%; the rates were 58.5% and 47.2% (p=0.100, ITT analysis) in the CO2 and air groups, respectively.CO2 insufflation improves the intubation depth and total enteroscopy rate in SBE with a good safety profile and acceptability compared with that of air, and thus is recommended for clinical utilisation.ClinicalTrial.gov identifier: NCT01758900.
- Published
- 2014
39. A genome wide association study of genetic loci that influence tumour biomarkers cancer antigen 19-9, carcinoembryonic antigen and α fetoprotein and their associations with cancer risk
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Jiang Chang, Tangchun Wu, Jielin Sun, Sheng Wei, Frank B. Hu, Aihua Tan, Gangqiao Zhou, Yuanfeng Li, Dongfeng Li, Xiaobo Yang, Junjie Feng, Youjie Wang, Yuan Liang, Huan Guo, Li Zhou, Mingjian Lang, Weimin Fang, Handong Yang, Yong Gao, Xiaomin Zhang, Lixuan Gui, Yunfeng He, Yingying Feng, Seong Tae Kim, Feng Wang, Haiying Zhang, Xinwen Min, Qifei Deng, Xue Qin, Jing Yuan, Yanling Hu, Shijun Zhang, Weihong Chen, Zengnan Mo, Jiang Zhu, Dongxin Lin, Xiaoping Miao, Sha Tao, Jianfeng Xu, S. Lilly Zheng, Xiayun Dai, Meian He, Tao Peng, Wen Tan, Chen Wu, Hongxing Zhang, Yi Li, Yun Zhai, Dianke Yu, and Kan Zhai
- Subjects
Oncology ,Adult ,Male ,medicine.medical_specialty ,China ,Carcinoma, Hepatocellular ,CA-19-9 Antigen ,Esophageal Neoplasms ,Single-nucleotide polymorphism ,Genome-wide association study ,Biology ,Polymorphism, Single Nucleotide ,Carcinoembryonic antigen ,Asian People ,Risk Factors ,ABO blood group system ,Internal medicine ,Pancreatic cancer ,Cancer screening ,medicine ,Biomarkers, Tumor ,Humans ,Aged ,Carcinoma ,Liver Neoplasms ,Gastroenterology ,Cancer ,Middle Aged ,medicine.disease ,digestive system diseases ,Carcinoembryonic Antigen ,Pancreatic Neoplasms ,Case-Control Studies ,biology.protein ,Carcinoma, Squamous Cell ,Linear Models ,CA19-9 ,Female ,alpha-Fetoproteins ,Genome-Wide Association Study - Abstract
Objective Tumour biomarkers are used as indicators for cancer screening and as predictors for therapeutic responses and prognoses in cancer patients. We aimed to identify genetic loci that influence concentrations of cancer antigen 19-9 (CA19-9), carcinoembryonic antigen (CEA) and α fetoprotein (AFP), and investigated the associations between the significant single nucleotide polymorphisms (SNPs) with risks of oesophageal squamous cell (OSCC), pancreatic and hepatocellular cancers. Design We carried out a genome wide association study on plasma CA19-9, CEA and AFP concentrations in 3451 healthy Han Chinese and validated the results in 10 326 individuals. Significant SNPs were further investigated in three case control studies (2031 OSCC cases and 2044 controls; 981 pancreatic cancer cases and 1991 controls; and 348 hepatocellular cancer cases and 359 controls). Results The analyses showed association peaks on three genetic loci for CA19-9 ( FUT6-FUT3 at 19p13.3, FUT2 - CA11 at 19q13.3 and B3GNT3 at 19p13.1; p=1.16×10 −13 –3.30×10 −290 ); four for CEA ( ABO at 9q34.2, FUT6 at 19p13.3, FUT2 at 19q13.3 and FAM3B at 21q22.3; p=3.33×10 −22 –5.81×10 −209 ); and two for AFP ( AFP at 4q11-q13 and HISPPD2A at 15q15.3; p=3.27×10 −18 and 1.28×10 −14 ). These explained 17.14% of the variations in CA19-9, 8.95% in CEA and 0.57% in AFP concentrations. Significant ABO variants were also associated with risk of OSCC and pancreatic cancers, and AFP variants with risk of hepatocellular cancer (p Conclusions This study identified several loci associated with CA19-9, CEA and AFP concentrations. The ABO variants were associated with risk of OSCC and pancreatic cancers and AFP variants with risk of hepatocellular cancer.
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- 2013
40. Gut microbial profile is altered in primary biliary cholangitis and partially restored after UDCA therapy.
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Ruqi Tang, Yiran Wei, Yanmei Li, Weihua Chen, Haoyan Chen, Qixia Wang, Fan Yang, Qi Miao, Xiao Xiao, Haiyan Zhang, Min Lian, Xiang Jiang, Jun Zhang, Qin Cao, Zhuping Fan, Maoying Wu, Dekai Qiu, Jing-Yuan Fang, Ansari, Aftab, and Gershwin, M. Eric
- Subjects
CHOLANGITIS ,AUTOIMMUNE diseases ,URSODEOXYCHOLIC acid ,ENTEROBACTERIACEAE ,DISEASE progression ,THERAPEUTICS - Published
- 2018
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41. OCT1 is a determinant of synbindin-related ERK signalling with independent prognostic significance in gastric cancer
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Qian, Jin, primary, Kong, Xuan, additional, Deng, Niantao, additional, Tan, Patrick, additional, Chen, Haoyan, additional, Wang, Jilin, additional, Li, Zhaoli, additional, Hu, Ye, additional, Zou, Weiping, additional, Xu, Jie, additional, and Fang, Jing-Yuan, additional
- Published
- 2014
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42. Gut microbial profile is altered in primary biliary cholangitis and partially restored after UDCA therapy
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Tang, Ruqi, Wei, Yiran, Li, Yanmei, Chen, Weihua, Chen, Haoyan, Wang, Qixia, Yang, Fan, Miao, Qi, Xiao, Xiao, Zhang, Haiyan, Lian, Min, Jiang, Xiang, Zhang, Jun, Cao, Qin, Fan, Zhuping, Wu, Maoying, Qiu, Dekai, Fang, Jing-Yuan, Ansari, Aftab, Gershwin, M Eric, and Ma, Xiong
- Abstract
ObjectiveA close relationship between gut microbiota and some chronic liver disorders has recently been described. Herein, we systematically performed a comparative analysis of the gut microbiome in primary biliary cholangitis (PBC) and healthy controls.DesignWe first conducted a cross-sectional study of 60 ursodeoxycholic acid (UDCA) treatment-naive patients with PBC and 80 matched healthy controls. Second, an independent cohort composed of 19 treatment-naive patients and 34 controls was used to validate the results. Finally, a prospective study was performed in a subgroup of 37 patients with PBC who underwent analysis before and after 6 months of UDCA treatment. Faecal samples were collected, and microbiomes were analysed by 16S ribosomal RNA gene sequencing.ResultsA significant reduction of within-individual microbial diversity was noted in PBC (p=0.03). A signature defined by decreased abundance of four genera and increased abundance of eight genera strongly correlated with PBC (area under curve=0.86, 0.84 in exploration and validation data, respectively). Notably, the abundance of six PBC-associated genera was reversed after 6 months of UDCA treatment. In particular, Faecalibacterium, enriched in controls, was further decreased in gp210-positive than gp210-negative patients (p=0.002). Of interest was the finding that the increased capacity for the inferred pathway, bacterial invasion of epithelial cells in PBC, highly correlated with the abundance of bacteria belonging to Enterobacteriaceae.ConclusionsThis study presents a comprehensive landscape of gut microbiota in PBC. Dysbiosis was found in the gut microbiome in PBC and partially relieved by UDCA. Our study suggests that gut microbiota is a potential therapeutic target and diagnostic biomarker for PBC.
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- 2018
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43. OCT1 is a determinant of synbindin-related ERK signalling with independent prognostic significance in gastric cancer.
- Author
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Jin Qian, Xuan Kong, Niantao Deng, Patrick Tan, Haoyan Chen, Jilin Wang, Zhaoli Li, Ye Hu, Weiping Zou, Jie Xu, and Jing-Yuan Fang
- Subjects
CELLULAR signal transduction ,STOMACH cancer ,STOMACH cancer treatment ,IMMUNOFLUORESCENCE ,TRANSCRIPTION factors ,GENETICS ,PROGNOSIS - Abstract
Objective Octamer transcription factor 1 (OCT1) was found to be expressed in intestinal metaplasia and gastric cancer (GC), but the exact roles of OCT1 in GC remain unclear. The objective of this study was to determine the functional and prognostic implications of OCT1 in GC. Design Expression of OCT1 was examined in paired normal and cancerous gastric tissues and the prognostic significance of OCT1 was analysed by univariate and multivariate survival analyses. The functions of OCT1 on synbindin expression and extracellular signal-regulated kinase (ERK) phosphorylation were studied in vitro and in xenograft mouse models. Results The OCT1 gene is recurrently amplified and upregulated in GC. OCT1 overexpression and amplification are associated with poor survival in patients with GC and the prognostic significance was confirmed by independent patient cohorts. Combining OCT1 overexpression with American Joint Committee on Cancer staging improved the prediction of survival in patients with GC. High expression of OCT1 associates with activation of the ERK mitogen-activated protein kinase signalling pathway in GC tissues. OCT1 functions by transactivating synbindin, which binds to ERK DEF domain and facilitates ERK phosphorylation by MEK. OCT1-synbindin signalling results in the activation of ERK substrates ELK1 and RSK, leading to increased cell proliferation and invasion. Immunofluorescent study of human GC tissue samples revealed strong association between OCT1 protein level and synbindin expression/ ERK phosphorylation. Upregulation of OCT1 in mouse xenograft models induced synbindin expression and ERK activation, leading to accelerated tumour growth in vivo. Conclusions OCT1 is a driver of synbindin-mediated ERK signalling and a promising marker for the prognosis and molecular subtyping of GC. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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44. Carbon dioxide insufflation improves the intubation depth and total enteroscopy rate in single-balloon enteroscopy: a randomised, controlled, double-blind trial.
- Author
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Xuan Li, Yun-Jia Zhao, Jun Dai, Xiao-Bo Li, Han-Bin Xue, Yao Zhang, Guang-Su Xiong, Kazuo Ohtsuka, Yun-Jie Gao, Qiang Liu, Yan Song, Jing-Yuan Fang, and Zhi-Zheng Ge
- Subjects
CARBON dioxide ,INSUFFLATION ,ENTEROSCOPY ,INTUBATION ,PATIENTS - Abstract
Objective The total enteroscopy rate of single-balloon enteroscopy (SBE) using air insufflation is not satisfactory, and whether carbon dioxide (CO
2 ) insufflation increases the total enteroscopy rate of SBE is unknown. This randomised controlled trial aimed to determine whether CO2 insufflation facilitates the intubation depth and total enteroscopy rate of SBE. Design A total of 214 eligible patients referred for SBE were randomised to receive either air or CO2 insufflation, and included in the intention-to-test (ITT) analysis. In addition, 199 patients in whom enteroscopy was completed were included in the per-protocol (PP) analysis. Both the patients and endoscopists were blinded, and the intubation depth and total enteroscopy rate were defined as the primary outcomes. Results The CO2 group showed a superiority of intubation in the ITT analysis (oral route: 323.8±64.2 vs 238.3±68.6 cm; anal route: 261.6±74.2 vs 174.7±62.1 cm, both p<0.001), and the total enteroscopy rate (34.9% vs 17.6%, p=0.006). Similar results were obtained in a PP analysis for both outcomes. In addition, in the PP analysis, the addition of circumference after the procedure was less in the CO2 group (0.8±0.6 vs 3.3±1.8 cm, p=0.005) for the oral route. No serious complications were reported. The overall percentage of procedures with significant pathological findings was 52.8%; the rates were 58.5% and 47.2% (p=0.100, ITT analysis) in the CO2 and air groups, respectively. Conclusions CO2 insufflation improves the intubation depth and total enteroscopy rate in SBE with a good safety profile and acceptability compared with that of air, and thus is recommended for clinical utilisation. Trial registration number ClinicalTrial.gov identifier: NCT01758900. [ABSTRACT FROM AUTHOR]- Published
- 2014
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45. A genome wide association study of genetic loci that influence tumour biomarkers cancer antigen 19-9, carcinoembryonic antigen and a fetoprotein and their associations with cancer risk.
- Author
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Meian He, Chen Wu, Jianfeng Xu, Huan Guo, Handong Yang, Xiaomin Zhang, Jielin Sun, Dianke Yu, Li Zhou, Tao Peng, Yunfeng He, Yong Gao, Jing Yuan, Qifei Deng, Xiayun Dai, Aihua Tan, Yingying Feng, Haiying Zhang, Xinwen Min, and Xiaobo Yang
- Subjects
TUMOR markers ,CARCINOEMBRYONIC antigen ,ANTIGENS ,BIOMARKERS ,ALPHA fetoproteins ,ESOPHAGEAL cancer risk factors - Abstract
Objective Tumour biomarkers are used as indicators for cancer screening and as predictors for therapeutic responses and prognoses in cancer patients. We aimed to identify genetic loci that influence concentrations of cancer antigen 19-9 (CA19-9), carcinoembryonic antigen (CEA) and a fetoprotein (AFP), and investigated the associations between the significant single nucleotide polymorphisms (SNPs) with risks of oesophageal squamous cell (OSCC), pancreatic and hepatocellular cancers. Design We carried out a genome wide association study on plasma CA19-9, CEA and AFP concentrations in 3451 healthy Han Chinese and validated the results in 10 326 individuals. Significant SNPs were further investigated in three case control studies (2031 OSCC cases and 2044 controls; 981 pancreatic cancer cases and 1991 controls; and 348 hepatocellular cancer cases and 359 controls). Results The analyses showed association peaks on three genetic loci for CA19-9 (FUT6-FUT3 at 19p13.3, FUT2-CA11 at 19q13.3 and B3GNT3 at 19p13.1; p=1.16×10
-13 -3.30×10-290 ); four for CEA (ABO at 9q34.2, FUT6 at 19p13.3, FUT2 at 19q13.3 and FAM3B at 21q22.3; p=3.33×10-22 -5.81×10-209 ); and two for AFP (AFP at 4q11-q13 and HISPPD2A at 15q15.3; p=3.27×10-18 and 1.28×10-14 ). These explained 17.14% of the variations in CA19-9, 8.95% in CEA and 0.57% in AFP concentrations. Significant ABO variants were also associated with risk of OSCC and pancreatic cancers, and AFP variants with risk of hepatocellular cancer (p<0.05). Conclusions This study identified several loci associated with CA19-9, CEA and AFP concentrations. The ABO variants were associated with risk of OSCC and pancreatic cancers and AFP variants with risk of hepatocellular cancer. [ABSTRACT FROM AUTHOR]- Published
- 2014
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46. The complicated dialogue between Helicobacter pylori and p53.
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Zhi-Hang Tao and Jing-Yuan Fang
- Subjects
HELICOBACTER pylori ,P53 antioncogene ,HELICOBACTER pylori infections ,DNA mismatch repair ,TUMOR suppressor genes - Published
- 2020
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47. The complicated dialogue between Helicobacter pyloriand p53
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Tao, Zhi-Hang and Fang, Jing-Yuan
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- 2020
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48. Several aspects in study design need further consideration.
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Cheng-Bei Zhou and Jing-Yuan Fang
- Subjects
TREATMENT of helicobacter pylori infections ,PROTON pump inhibitors ,STOMACH cancer risk factors - Published
- 2018
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49. Several aspects in study design need further consideration
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Zhou, Cheng-Bei and Fang, Jing-Yuan
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- 2018
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50. Multiple balloon-like lesions in the small intestine of an adult with chronic diarrhoea
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Zhou, Cheng-Bei, Lu, Hong, Chen, Ying-Xuan, and Fang, Jing-Yuan
- Abstract
Clinical presentationA 28-year-old woman presented with a 3-year history of chronic watery diarrhoea along with abdominal pain and bloating, which could mostly be alleviated after defecation. Her symptom of diarrhoea, at least three times a day, could be relieved by neither probiotics nor antidiarrhoeal agents. She had also lost 5 kg in the last month. She denied family history, poor vaccine responses or significant infections in early childhood except for an allergy history to intravenous immunoglobulin (Ig) with immediate dyspnoea, palpitations and hypotension. Laboratory investigations suggested that the stool specimens were negative for viruses, parasites or bacteria. Laboratory evaluation revealed a low serum globulin level, 14.5 (reference range, 20–30 g/L); serum Ig levels were significantly abnormal: IgA <0.27 (0.7–4 g/L), IgM 0.24 (0.4–2.3 g/L), IgG 1.3 (7–16 g/L); white cell count 15.4×109/L (3.69–9.16×109/L); C-reactive protein (CRP) 20.5 (normal <10 mg/L); CD4+ lymphocyte/CD8+ lymphocyte 1.09% (1.5%–2%). Other laboratory findings were unremarkable, for example, tumour markers, autoantibodies and HIV, and so on. CT showed mesenteric nodule-like images and thickening of the wall and mucosa in small intestine. Peroral and transanal enteroscopy respectively demonstrated swelling mucosa and continuous granular lesions from duodenum to middle jejunum, and from middle ileum to terminal ileum (figure 1A–D).[Figure]QuestionWhat is the most likely diagnosis?
- Published
- 2019
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