Search

AIM: To observe the expression levels of pigment epithelium-derived factor(PEDF)and vascular endothelial growth factor(VEGF)in lens epithelial cells(LECs)of diabetic patients with age-related cataract, and explore the pathogenesis of diabetic with age-related cataract.METHODS: Retrospectively study. A total of 30 patients with age-related cataract(ARC group)and 30 patients with type 2 diabetes mellitus combined with age-related cataract(DC group)who treated at the Department of Ophthalmology of the First Affiliated Hospital of Bengbu Medical College from August 2020 to April 2021 were collected. Anterior capsule specimens with a diameter of 5.5-6.0mm in the central region of the intraoperative eye were collected during phacoemulsification. The protein expression levels of PEDF and VEGF in LECs were detected by Western-blot. Quantitative real-time PCR(qRT-PCR)was used to detect the relative mRNA expression levels of PEDF and VEGF.RESULTS:PEDF and VEGF were expressed in LECs of both groups, and the relative expression of VEGF mRNA in DC group was 1.364±0.062, which was higher than that of ARC group(1.000±0.0; P

High myopia is one of the major blinding diseases in China and even in the world, and high myopia with cataract is a kind of complex cataract with a high risk of blindness. At present, surgery is the only treatment. Because high myopia can lead to a series of complex changes in the eye, compared with the normal axial eye, it is easier to produce the refractive error and refractive drift after surgery. In this paper, the influencing factors of refractive error after cataract surgery for high myopia are reviewed, including the accuracy of preoperative biological measurement, the choice of intraocular lens calculation formula, and the change of effective intraocular lens position.

The global incidence of high myopia is increasing year by year. High myopia is closely related to cataract, but high myopia is accompanied by special pathological changes,which make the operative difficulty and the incidence of postoperative complications of high myopia with cataract is much higher than common cataract. With the advent of the era of refractive cataract, postoperative visual quality requirements of patients are higher, and the challenges to ophthalmologists are also greater. Therefore, we need to pay more attention to the management of patients with high myopia combined with cataract. In this paper, the surgical methods, preoperative biological measurements, common complications during and after the operation of high myopia with cataract were reviewed.

AIM: To research the expressions of B-cell lymphoma gene 2(Bcl-2), Bcl-2 associated X-protein(Bax)and Ki67(proliferating cell marker)in the primary pterygium and normal bulbar conjunctiva, to explore the pathogenesis of pterygium. METHODS: Thirty pterygium were surgically removed and 5 normal bulbar conjunctivas were also obtained. Formalin-fixed, paraffin-wax-embedded tissues were analyzed by immunohistochemistry with Bcl-2, Bax, Ki67. RESULTS: The mean positive expression of Bcl-2, Bax and Ki67 in the primary pterygium was 14.73%, 7.00% and 12.73%, respectively 4.00%, 6.00% and 0 in normal conjunctiva group. The expression of Bcl-2 and Ki67 in the head of primary pterygium was higher than that of neck and body. CONCLUSION: Bcl-2 and Ki67 have highly expressions in the primary pterygium, the expressions in the head of primary pterygium is the highest, the expressions in normal bulbar conjunctiva is low or no. There is no significant difference in the expression of Bax in the primary pterygium and normal bulbar conjunctiva. Apoptosis imbalance and abnormal proliferation of cells may be one of the mechanisms for the occurrence and development of the primary pterygium.

Cataract is the first ophthalmological blinding eye disease. In recent years, phacoemulsification has become the preferred method for the treatment of cataract due to its short time and small damage, which is widely used by ophthalmologists. With the continuous improvement of technology, serious complications caused by phacoemulsification become less and less. But the phenomenon of postoperative corneal edema is not uncommon. The incidence of corneal edema is especially high in patients with diabetic cataracts. This paper reviewes the concept of corneal edema, pathogenesis and the related factors of corneal edema after phacoemulsification in diabetic cataract patients.

Pterygium is one of the most common ocular surface diseases. Its exact etiology and pathogenesis are not completely understood. At present, it is considered that its occurrence and development is the result of many factors.Current studies have indicated that the occurrence of pterygium is closely related to the environmental factors.Long time exposure to sunlight,dust, pollen and other long-term chronic stimulation are the main incentive factors.Various factors have caused limbal barrier dysfunction, induced the level of a variety of growth factors and inflammatory factors increased, so that the conjunctival tissue degenerate and proliferate to the cornea in the formation of pterygium. In this paper, the research progress of the pathogenesis of pterygium is reviewed.

AIM:To detect oxidative DNA damage marker 8-hydroxy-2-deoxyguanosine(8-OHdG)in primary pterygium and normal conjunctival tissues, explore the role of oxidative DNA damage in the pathogenesis of pterygium. METHODS: Totally 35 primary pterygium specimens were collected during surgery and 5 normal conjunctival specimens which above the normal temporal bulbar conjunctiva were collected. The expressions of 8-OHdG in pterygium tissues were detected by immunohistochemical method and compared with the normal conjunctival tissues. The difference of 8-OHdG expression between the two groups was compared. RESULTS: There were 24(69%)pterygium specimens positive for 8-OHdG staining, limited to the nuclei of the epithelial layer. No substantial staining was visible in the subepithelial fibrovascular layers. All normal controls were negative for 8-OHdG staining. The difference of 8-OHdG expression between the two groups was statistically significant(P=0.007). CONCLUSION: The increased levels of 8-OHdG in the pterygium tissues indicate that oxidative DNA damage maybe play an important role in the pathogenesis of pterygium.