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The effects of xylose, xylitol on enzymolysis of bagasse xylan by xylanase Shearzyme 500 L were studied. Xylooligosaccharide with high xylobiose content was obtained through the enzymolysis of by-product xylose by Candida tropiclis and relieving the inhibitory effect of xylitol on xylanse. The results were as follows: xylose was the inhibitor of Shearzyme 500 L,and its inhibitory effect was in proportion to xylose content in solution; xylitol had no inhibitory effect on xylanase; C. tropiclis could transform the xylose in the solution of bagasse xylan into xylitol without xylooligosaccharide, and xylobiose increased from 53.09% to 62.92% in total sugar, and reached 78.90% through the second hydrolysis. [ABSTRACT FROM AUTHOR]