1. Microglia replenished OHSC: A culture system to studyin vivolike adult microglia
- Author
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Rianne van der Pijl, Lisa Füner, Erik Boddeke, Annette Masuch, Bart J. L. Eggen, Yochai Wolf, and Knut Biber
- Subjects
0301 basic medicine ,Cell type ,Microglia ,Central nervous system ,Excitotoxicity ,Biology ,medicine.disease_cause ,Neuroprotection ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Tissue culture ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,nervous system ,Neurology ,In vivo ,CX3CR1 ,medicine ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Recent data suggest that ramified microglia fulfil various tasks in the brain. However, to investigate this unique cell type cultured primary microglia are only a poor model. We here describe a method to deplete and repopulate organotypic hippocampal slice cultures (OHSC) with ramified microglia isolated from adult mouse brain creating microglia-replenished OHSC (Mrep-OHSC). Replenished microglia integrate into the tissue and ramify to a degree indistinguishable from their counterparts in the mouse brain. Moreover, wild-type slices replenished with microglia from TNFα-deficient animals provide similar results as OHSC prepared from microglia-specific TNFα-knockout mice (CX3CR1(cre) /TNFα(fl/fl) ). Furthermore, this study demonstrates that replenished microglia in OHSC maintain original functions and properties acquired in vivo. Microglia from ERCC1(Δ/ko) mice, a mouse model of accelerated aging, maintain enhanced Mac2 expression and their activated phenotype after replenishment to wild-type OHSC tissue. Thus, the present study demonstrates that Mrep-OHSC are a unique tool to construct chimeric brain slices allowing studying the function of different phenotypes of in vivo like microglia in a tissue culture setting. GLIA 2016 GLIA 2016;64:1285-1297.
- Published
- 2016