Summary: In this article, there will be presented the main aspects of the radiation protection for a site dedicated to the production of radioisotopes by cyclotron for medical use. After analyzing the design parameters of the site for the operation from a point of view of the radiation protection of the operators, the population and the environment (shielding around the accelerator, ventilation system, chimney releases etc.), we will focus our attention to the handling of unsealed sources produced, with particular regard to the operating procedures. The final part will be dedicated to intervention in exceptional operating conditions (entring the bunker due to a machine failure, breaking of a vial during transfer from the hot cell to the transport container and accidental contamination by an operator)., Competing Interests: The authors of this article have no conflict of interests to disclose., (Copyright© by GIMLE.)