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Start Over Author "ZHANG Wenwei" Journal gigascience
6 results on '"ZHANG Wenwei"'

1. Erratum to: Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing

Author

Mak, Sarah Siu Tze, primary, Gopalakrishnan, Shyam, additional, Carøe, Christian, additional, Geng, Chunyu, additional, Liu, Shanlin, additional, Sinding, Mikkel-Holger S, additional, Kuderna, Lukas F K, additional, Zhang, Wenwei, additional, Fu, Shujin, additional, Vieira, Filipe G, additional, Germonpré, Mietje, additional, Bocherens, Hervé, additional, Fedorov, Sergey, additional, Petersen, Bent, additional, Sicheritz-Pontén, Thomas, additional, Marques-Bonet, Tomas, additional, Zhang, Guojie, additional, Jiang, Hui, additional, and Gilbert, M Thomas P, additional

Published
2018
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2. Erratum to: A reference human genome dataset of the BGISEQ-500 sequencer

Author

Huang, Jie, primary, Liang, Xinming, additional, Xuan, Yuankai, additional, Geng, Chunyu, additional, Li, Yuxiang, additional, Lu, Haorong, additional, Qu, Shoufang, additional, Mei, Xianglin, additional, Chen, Hongbo, additional, Yu, Ting, additional, Sun, Nan, additional, Rao, Junhua, additional, Wang, Jiahao, additional, Zhang, Wenwei, additional, Chen, Ying, additional, Liao, Sha, additional, Jiang, Hui, additional, Liu, Xin, additional, Yang, Zhaopeng, additional, Mu, Feng, additional, and Gao, Shangxian, additional

Published
2018
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3. Assessment of the cPAS-based BGISEQ-500 platform for metagenomic sequencing

Author

Fang, Chao, primary, Zhong, Huanzi, additional, Lin, Yuxiang, additional, Chen, Bing, additional, Han, Mo, additional, Ren, Huahui, additional, Lu, Haorong, additional, Luber, Jacob M, additional, Xia, Min, additional, Li, Wangsheng, additional, Stein, Shayna, additional, Xu, Xun, additional, Zhang, Wenwei, additional, Drmanac, Radoje, additional, Wang, Jian, additional, Yang, Huanming, additional, Hammarström, Lennart, additional, Kostic, Aleksandar D, additional, Kristiansen, Karsten, additional, and Li, Junhua, additional

Published
2017
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4. Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing

Author

Mak, Sarah Siu Tze, primary, Gopalakrishnan, Shyam, additional, Carøe, Christian, additional, Geng, Chunyu, additional, Liu, Shanlin, additional, Sinding, Mikkel-Holger S, additional, Kuderna, Lukas F K, additional, Zhang, Wenwei, additional, Fu, Shujin, additional, Vieira, Filipe G, additional, Germonpré, Mietje, additional, Bocherens, Hervé, additional, Fedorov, Sergey, additional, Petersen, Bent, additional, Sicheritz-Pontén, Thomas, additional, Marques-Bonet, Tomas, additional, Zhang, Guojie, additional, Jiang, Hui, additional, and Gilbert, M Thomas P, additional

Published
2017
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5. A reference human genome dataset of the BGISEQ-500 sequencer

Author

Huang, Jie, primary, Liang, Xinming, additional, Xuan, Yuankai, additional, Geng, Chunyu, additional, Li, Yuxiang, additional, Lu, Haorong, additional, Qu, Shoufang, additional, Mei, Xianglin, additional, Chen, Hongbo, additional, Yu, Ting, additional, Sun, Nan, additional, Rao, Junhua, additional, Wang, Jiahao, additional, Zhang, Wenwei, additional, Chen, Ying, additional, Liao, Sha, additional, Jiang, Hui, additional, Liu, Xin, additional, Yang, Zhaopeng, additional, Mu, Feng, additional, and Gao, Shangxian, additional

Published
2017
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6. Assessment of the cPAS-based BGISEQ-500 platform for metagenomic sequencing.

Author

Fang C, Zhong H, Lin Y, Chen B, Han M, Ren H, Lu H, Luber JM, Xia M, Li W, Stein S, Xu X, Zhang W, Drmanac R, Wang J, Yang H, Hammarström L, Kostic AD, Kristiansen K, and Li J

Subjects
Bacteria classification, Computational Biology, Feces microbiology, High-Throughput Nucleotide Sequencing methods, Humans, Bacteria genetics, Gastrointestinal Microbiome genetics, Metagenomics methods, Sequence Analysis, DNA methods
Abstract

Background: More extensive use of metagenomic shotgun sequencing in microbiome research relies on the development of high-throughput, cost-effective sequencing. Here we present a comprehensive evaluation of the performance of the new high-throughput sequencing platform BGISEQ-500 for metagenomic shotgun sequencing and compare its performance with that of 2 Illumina platforms., Findings: Using fecal samples from 20 healthy individuals, we evaluated the intra-platform reproducibility for metagenomic sequencing on the BGISEQ-500 platform in a setup comprising 8 library replicates and 8 sequencing replicates. Cross-platform consistency was evaluated by comparing 20 pairwise replicates on the BGISEQ-500 platform vs the Illumina HiSeq 2000 platform and the Illumina HiSeq 4000 platform. In addition, we compared the performance of the 2 Illumina platforms against each other. By a newly developed overall accuracy quality control method, an average of 82.45 million high-quality reads (96.06% of raw reads) per sample, with 90.56% of bases scoring Q30 and above, was obtained using the BGISEQ-500 platform. Quantitative analyses revealed extremely high reproducibility between BGISEQ-500 intra-platform replicates. Cross-platform replicates differed slightly more than intra-platform replicates, yet a high consistency was observed. Only a low percentage (2.02%-3.25%) of genes exhibited significant differences in relative abundance comparing the BGISEQ-500 and HiSeq platforms, with a bias toward genes with higher GC content being enriched on the HiSeq platforms., Conclusions: Our study provides the first set of performance metrics for human gut metagenomic sequencing data using BGISEQ-500. The high accuracy and technical reproducibility confirm the applicability of the new platform for metagenomic studies, though caution is still warranted when combining metagenomic data from different platforms.

Published
2018
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