1. Soil microaggregates reveal different physical structure, microbial diversity, and organic matter composition depending on clay content and isolation method.
- Author
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Puschmann, Daniel Uteau, Felde, Vincent J.M.N.L., Schweizer, Steffen A., Biesgen, Danh, Ulbrich, Angela, Knief, Claudia, Graf-Rosenfellner, Markus, and Kögel-Knabner, Ingrid
- Subjects
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MICROBIAL diversity , *CLAY soils , *ORGANIC compounds , *SANDY loam soils , *CLAY , *SOIL composition , *SOIL texture - Abstract
Most of our knowledge about the aggregation of soil components into larger structures is derived from analyzing wet-sieved samples, which impedes the quantification of mechanical aggregate stability. Here, we present a dry method to isolate soil microaggregates (< 250 µm) by uniaxial crushing with the goal to preserve their natural structure. To understand how soil texture governs soil microaggregation, we investigated topsoil samples of an agricultural plot in Scheyern (Germany) with clay contents of 19, 24, and 34 %. Microscopic and tomographic observations revealed fine particle microaggregates in the clayey soils, whereas the dry-crushed aggregates in the sandy loam soil included sand grains. These dry-crushed sandy microaggregates showed a high variability when measuring their uniaxial mechanical stability in a loading frame. We compared the dry-crushed microaggregates with wet-sieved ones and found that wet-sieved aggregates had a lower and less variable mechanical stability indicating a more homogeneous structure and fewer occluded sand grains. The more heterogeneous arrangements of dry-crushed aggregates also revealed a higher microbial diversity than the wet-sieved ones. In the dry-crushed aggregates the microbial community composition differed between clay contents, whereas it was similar between small (20-53 µm) and large (53-250 µm) microaggregates. When dispersing the dry-crushed aggregates in water we found mostly aggregates sized approximately 25 µm in diameter. The distribution of such fine particle microaggregates was related with higher concentrations of organic matter in aggregates. Within the dry-crushed microaggregates, the larger size fraction retained more organic matter that was less decomposed than in the smaller size fractions. Our study reveals that dry-crushing enables the isolation of sandy microaggregates, whereas water-stable fine particle microaggregates governed the distribution of organic matter. [ABSTRACT FROM AUTHOR]
- Published
- 2019