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Your search keyword '"Shelley J. Russek"' showing total 2 results
Start Over Author "Shelley J. Russek" Journal genomics
2 results on '"Shelley J. Russek"'

1. Mapping of the α4 subunit gene (GABRA4) to human chromosome 4 defines an α2—α4—β1—γ1 gene cluster: further evidence that modern GABAA receptor gene clusters are derived from an ancestral cluster

Author

David H. Farb, Pamela J. McLean, and Shelley J. Russek

Subjects
Chromosome 7 (human), Genetics, Chromosomes, Human, Pair 15, Base Sequence, Molecular Sequence Data, GABRA4, Chromosome Mapping, Locus (genetics), DNA, Biology, Receptors, GABA-A, Biological Evolution, Polymerase Chain Reaction, Chromosome 4, Gene mapping, Multigene Family, Gene duplication, Gene cluster, biology.protein, Chromosomes, Human, Pair 5, Humans, Amino Acid Sequence, Chromosomes, Human, Pair 4, G alpha subunit
Abstract

We demonstrated previously that an alpha 1-beta 2-gamma 2 gene cluster of the gamma-aminobutyric acid (GABAA) receptor is located on human chromosome 5q34-q35 and that an ancestral alpha-beta-gamma gene cluster probably spawned clusters on chromosomes 4, 5, and 15. Here, we report that the alpha 4 gene (GABRA4) maps to human chromosome 4p14-q12, defining a cluster comprising the alpha 2, alpha 4, beta 1, and gamma 1 genes. The existence of an alpha 2-alpha 4-beta 1-gamma 1 cluster on chromosome 4 and an alpha 1-alpha 6-beta 2-gamma 2 cluster on chromosome 5 provides further evidence that the number of ancestral GABAA receptor subunit genes has been expanded by duplication within an ancestral gene cluster. Moreover, if duplication of the alpha gene occurred before duplication of the ancestral gene cluster, then a heretofore undiscovered subtype of alpha subunit should be located on human chromosome 15q11-q13 within an alpha 5-alpha x-beta 3-gamma 3 gene cluster at the locus for Angelman and Prader-Willi syndromes.

Published
1995

2. Mapping of the β2 Subunit Gene (GABRB2) to Microdissected Human Chromosome 5q34-q35 Defines a Gene Cluster for the Most Abundant GABAA Receptor Isoform

Author

Shelley J. Russek and David H. Farb

Subjects
Gene isoform, Macromolecular Substances, Molecular Sequence Data, Hybrid Cells, Biology, Polymerase Chain Reaction, Mice, Gene mapping, Molecular evolution, Gene cluster, Gene expression, Genetics, Animals, Humans, Amino Acid Sequence, Gene, Conserved Sequence, DNA Primers, Regulation of gene expression, Base Sequence, Sequence Homology, Amino Acid, Intron, Chromosome Mapping, Hominidae, Exons, Receptors, GABA-A, Biological Evolution, Introns, Rats, Multigene Family, Chromosomes, Human, Pair 5, Chickens
Abstract

The gamma-aminobutyric acid receptor (GABAAR) is a multisubunit Cl- channel that mediates most fast inhibitory synaptic transmission in the central nervous system. Molecular evolution has given rise to many genetic variants of GABAAR subunits, including alpha 1-6, beta 1-4, gamma 1-4, delta, and rho 1-2, suggesting that an enormous number of combinations of subunits are possible. Here we report that the beta 2 gene is located on chromosome 5q34-q35, defining a cluster comprising alpha 1, beta 2, and gamma 2 genes that together code for the most abundant GABAAR isoform. The fact that intron position is conserved in the beta 1-3 genes, taken together with the observation that chromosomes 4 and 15 also contain distinct alpha-beta-gamma gene clusters, strongly suggests that an ancestral alpha-beta-gamma cluster was duplicated and translocated to at least two different chromosomes. This organization of GABAAR gene clusters may have been preserved as linkage provides a mechanism for facilitating coordinate gene expression.

Published
1994

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