1. Sequences from a family of bovine Y-chromosomal repeats
- Author
-
Margaret Ellen Matthews and Ken C. Reed
- Subjects
clone (Java method) ,Male ,Restriction Mapping ,Molecular cloning ,Biology ,Y chromosome ,Restriction map ,Species Specificity ,Y Chromosome ,Genetics ,Animals ,Cloning, Molecular ,Repeated sequence ,Genomic organization ,Southern blot ,Repetitive Sequences, Nucleic Acid ,Cloning ,Sheep ,Deer ,Goats ,DNA ,Molecular biology ,Blotting, Southern ,Cattle ,Female - Abstract
A 307-bp Sau3AI fragment previously cloned by deletion enrichment from the bovine Y chromosome was used to isolate a larger lambda EMBL3A genomic cattle clone. The whole 13-kb insert did not give a sex-specific pattern of hybridization to Southern blots of cattle DNA. Subclones from this phage, however, did show that this fragment had a Y-chromosomal origin. It was estimated that at least 40% of the cattle Y chromosome is composed of repeated sequences related to those within these subcloned fragments. Sequences within these subclones are male-specific or male-enriched also in sheep, goats, and deer. Comparison of cattle and sheep homologues of these sequences reveals that much greater amplification and rearrangement have occurred on the cattle Y chromosome than on the sheep Y. The apparent insertion of sequences into cattle Y-specific sequences relative to the sheep homologues suggests possible mechanisms for the evolution of the artiodactyl Y chromosome.
- Published
- 1992