1. Regional localization of the human G protein αi2 (GNAI2) gene: Assignment to 3p21 and a related sequence (GNAI2L) to 12p12–p13
- Author
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Jon G. Seidman, Cynthia C. Morton, Ivana Magovcevic, Cynthia Tolman, Siew-Lan Ang, and Eva J. Neer
- Subjects
Molecular Sequence Data ,Hybrid Cells ,Biology ,Polymerase Chain Reaction ,Mice ,Gene mapping ,GTP-Binding Proteins ,Chromosome regions ,Genetics ,Animals ,Humans ,Chromosome 12 ,Chromosome 7 (human) ,Chromosomes, Human, Pair 12 ,Base Sequence ,Chromosome Mapping ,Nucleic Acid Hybridization ,DNA ,Molecular biology ,Chromosome Banding ,Chromosome 17 (human) ,Chromosome 3 ,Chromosomes, Human, Pair 3 ,Peptides ,Chromosome 21 ,Chromosome 22 - Abstract
Gi alpha proteins, members of the G protein signal transduction family, include a small number of polypeptides: Gi alpha 1 (GNAI1), Gi alpha 2 (GNAI2), and Gi alpha 3 (GNAI3). A cDNA for the human GNAI2 gene has been isolated from a human T-cell library and is mapped by chromosomal in situ hybridization to the short arm of chromosome 3 at 3p21. A related sequence, GNAI2L, is mapped by in situ hybridization to the short arm of chromosome 12 at p12-p13. These mapping results are further supported by amplification of GNAI2-specific sequences in a monochromosomal human/rodent somatic cell hybrid containing only human chromosome 3. Of note, these assignments are to chromosome regions in which other G proteins reside. Localization of GNAI2 to 3p21 is of great interest as this region of the short arm of chromosome 3 is frequently involved in rearrangements in various human tumors.
- Published
- 1992
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