1. Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection
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Herbert B. Tanowitz, Shankar Mukherjee, João Santana da Silva, Aparna Mukhopadhyay, Chris Albanese, Michael P. Lisanti, Fabiana S. Machado, Linda A. Jelicks, Fnu Nagajyothi, Fangxia Guan, Antonio Carlos Carvalho, Thomas J. Belbin, Louis M. Weiss, and David C. Spray
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Chagas Cardiomyopathy ,Male ,Chagas disease ,Cardiomyopathy ,Trypanosoma cruzi ,Article ,Pathogenesis ,Mice ,Gene expression ,parasitic diseases ,medicine ,Genetics ,Animals ,Cluster Analysis ,Oligonucleotide Array Sequence Analysis ,Cyclins ,Regulation of gene expression ,Infection/inflammation ,biology ,Microarray analysis techniques ,Gene Expression Profiling ,Myocardium ,Gene array analysis ,Fas ,biology.organism_classification ,medicine.disease ,Virology ,Gene expression profiling ,Gene Expression Regulation ,Models, Animal ,Immunology - Abstract
Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite, Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in the gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with an oppositely labeled flip array. We identified eight district categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of Chagasic cardiomyopathy and provide new targets for intervention.
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