1. Recovery of a function involving gene duplication by retroposition in Saccharomyces cerevisiae
- Author
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Jacky de Montigny, Yves Tourrette, Jean-Luc Souciet, Joseph Schacherer, Serge Potier, Génétique moléculaire, génomique, microbiologie (GMGM), and Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS)
- Subjects
MESH: Multie ,Saccharomyces cerevisiae Proteins ,Retroelements ,Transcription, Genetic ,Sequence analysis ,MESH: Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) ,5' Flanking Region ,5' flanking region ,Saccharomyces cerevisiae ,Genes, Fungal ,Biology ,MESH: Aspartate Carbamoyltransferase ,MESH: 3' Flanking Region ,Transcription (biology) ,Multienzyme Complexes ,Gene Duplication ,Gene Expression Regulation, Fungal ,Gene duplication ,Genetics ,Aspartate Carbamoyltransferase ,3' Flanking Region ,Letters ,DNA, Fungal ,Gene ,Genetics (clinical) ,Regulation of gene expression ,MESH: 5' Flanking Region ,Recombination, Genetic ,[SDV.GEN]Life Sciences [q-bio]/Genetics ,MESH: Gene Duplication ,Fungal genetics ,Chromosome Mapping ,Sequence Analysis, DNA ,biology.organism_classification ,MESH: Chromosomes, Fungal ,MESH: DNA, Fungal ,Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) ,Chromosomes, Fungal ,MESH: Chromosome Mapping ,MESH: Genes, Fungal ,MESH: Gene Expression Regulation, Fungal - Abstract
The duplication of DNA sequences contributes to genomic plasticity and is known to be one of the key factors responsible for evolution. The mechanisms underlying these rare events, which have been frequently mentioned by authors performing genomic analysis, have not yet been completely elucidated. These mechanisms were approached here in the yeast Saccharomyces cerevisiae, using a positive selection screen based on a particular mutated allele of the URA2 gene. Spontaneous revertants containing a duplication of the terminal part of the URA2 gene were selected and analyzed. Some important features of the duplicated regions, such as their chromosome location, size, and insertion sites, were characterized. The events selected correspond to a single inter- or intrachromosomal gene duplication process. The duplicated ATCase sequence is generally punctuated by a poly(A) tract and is always located in Ty1 sequences. In addition, the activation of a Ty1 transcription process increased the frequency of the duplication events. All in all, these data suggest that the duplication mechanism involves the reverse transcription of mRNA and the subsequent integration of the cDNA into a Ty1 area. The Ty1 elements and the retrotransposon-encoded function are key factors contributing to chromosomal reshaping. The genomic rearrangements described constitute experimental evidence for the recovery of a function involving duplication by retroposition.
- Published
- 2004
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