1. A multiplicity of factors contributes to selective RNA polymerase III occupancy of a subset of RNA polymerase III genes in mouse liver
- Author
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Mauro Delorenzi, Irina Krier, Teemu Andersin, Li Long, Nicolas Guex, Arnaud Fortier, David Bernasconi, Marion Leleu, Guillaume Rey, Julia Cajan, Fabrice P. A. David, Winship Herr, Fabienne Lammers, Sunil K. Raghav, Olivier Martin, Jacques Rougemont, Aurélien Naldi, Roberto Fabbretti, Eugenia Migliavacca, Pascal Gos, Viviane Praz, Robin Liechti, Ueli Schibler, Gwendal LeMartelot, Nouria Hernandez, Laura Symul, Pascal Cousin, Frederick J. Ross, Yohan Jarosz, Béatrice Desvergne, Donatella Canella, Nacho Molina, Ioannis Xenarios, Felix Naef, Lucas Sinclair, Volker Vlegel, Federica Gilardi, Gwendal Le Martelot, Bart Deplancke, Dmitry Kuznetsov, University of Zurich, Delorenzi, Mauro, CycliX Consortium, Hernandez, N., Delorenzi, M., Deplancke, B., Desvergne, B., Guex, N., Herr, W., Naef, F., Rougemont, J., Schibler, U., Andersin, T., Cousin, P., Gilardi, F., Gos, P., Le Martelot, G., Lammers, F., Canella, D., Raghav, S., Fabbretti, R., Fortier, A., Long, L., Vlegel, V., Xenarios, I., Migliavacca, E., Praz, V., David, F., Jarosz, Y., Kuznetsov, D., Liechti, R., Martin, O., Ross, F., Sinclair, L., Cajan, J., Krier, I., Leleu, M., Molina, N., Naldi, A., Rey, G., Symul, L., and Bernasconi, D.
- Subjects
Male ,Chromatin Immunoprecipitation ,2716 Genetics (clinical) ,Pseudogene ,genetic processes ,Biology ,RNA polymerase III ,Mice ,chemistry.chemical_compound ,RNA, Transfer ,SX00 SystemsX.ch ,1311 Genetics ,Transcription (biology) ,RNA polymerase ,Gene expression ,Genetics ,Animals ,Humans ,SX04 CycliX ,Gene ,Genetics (clinical) ,Oligonucleotide Array Sequence Analysis ,Models, Genetic ,Gene Expression Profiling ,Research ,RNA Polymerase III ,RNA ,Genomics ,Sequence Analysis, DNA ,Molecular biology ,Mice, Inbred C57BL ,enzymes and coenzymes (carbohydrates) ,Liver ,chemistry ,Transfer RNA ,570 Life sciences ,biology ,bacteria ,Chromatin Immunoprecipitation/methods ,Genomics/methods ,Liver/metabolism ,RNA Polymerase III/genetics ,RNA Polymerase III/metabolism ,RNA, Transfer/genetics ,RNA, Transfer/metabolism ,Sequence Analysis, DNA/methods - Abstract
The genomic loci occupied by RNA polymerase (RNAP) III have been characterized in human culture cells by genome-wide chromatin immunoprecipitations, followed by deep sequencing (ChIP-seq). These studies have shown that only ∼40% of the annotated 622 human tRNA genes and pseudogenes are occupied by RNAP-III, and that these genes are often in open chromatin regions rich in active RNAP-II transcription units. We have used ChIP-seq to characterize RNAP-III-occupied loci in a differentiated tissue, the mouse liver. Our studies define the mouse liver RNAP-III-occupied loci including a conserved mammalian interspersed repeat (MIR) as a potential regulator of an RNAP-III subunit-encoding gene. They reveal that synteny relationships can be established between a number of human and mouse RNAP-III genes, and that the expression levels of these genes are significantly linked. They establish that variations within the A and B promoter boxes, as well as the strength of the terminator sequence, can strongly affect RNAP-III occupancy of tRNA genes. They reveal correlations with various genomic features that explain the observed variation of 81% of tRNA scores. In mouse liver, loci represented in the NCBI37/mm9 genome assembly that are clearly occupied by RNAP-III comprise 50 Rn5s (5S RNA) genes, 14 known non-tRNA RNAP-III genes, nine Rn4.5s (4.5S RNA) genes, and 29 SINEs. Moreover, out of the 433 annotated tRNA genes, half are occupied by RNAP-III. Transfer RNA gene expression levels reflect both an underlying genomic organization conserved in dividing human culture cells and resting mouse liver cells, and the particular promoter and terminator strengths of individual genes.
- Published
- 2012