Search

Your search keyword '"Victoria A. Sleight"' showing total 2 results
Start Over Author "Victoria A. Sleight" Journal genome biology
2 results on '"Victoria A. Sleight"'

1. ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Author

Helena García-Castro, Nathan J. Kenny, Marta Iglesias, Patricia Álvarez-Campos, Vincent Mason, Anamaria Elek, Anna Schönauer, Victoria A. Sleight, Jakke Neiro, Aziz Aboobaker, Jon Permanyer, Manuel Irimia, Arnau Sebé-Pedrós, and Jordi Solana

Subjects
Single-cell transcriptomics, Planarian, RNA-seq, Fixation, Dissociation, SPLiT-seq, Biology (General), QH301-705.5, Genetics, QH426-470
Abstract

Abstract Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life.

Published
2021
Full Text
View/download PDF

2. ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Author

Anna Schönauer, A. Aziz Aboobaker, Anamaria Elek, Helena García-Castro, Vincent Mason, Jon Permanyer, Patricia Álvarez-Campos, Jordi Solana, Victoria A. Sleight, Nathan J. Kenny, Jakke Neiro, Arnau Sebé-Pedrós, Manuel Irimia, Marta Iglesias, and UAM. Departamento de Biología

Subjects
lcsh:QH426-470, Single cell transcriptomics, Method, Computational biology, Biology, Dissociation (chemistry), Combinatorial Indexing, Workflow, 03 medical and health sciences, 0302 clinical medicine, Combinatorial indexing, Single-Cell Transcriptomics, Animals, Zoología, lcsh:QH301-705.5, 030304 developmental biology, Cryopreservation, 0303 health sciences, Planarian, Sequence Analysis, RNA, Gene Expression Profiling, High-Throughput Nucleotide Sequencing, Planarians, Biología y Biomedicina / Biología, Fixation, lcsh:Genetics, lcsh:Biology (General), RNA-seq, Single-Cell Analysis, Cell fixation, Transcriptome, 030217 neurology & neurosurgery, Single-cell transcriptomics, Dissociation, SPLiT-seq, Genètica
Abstract

Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life. This work was supported by an MRC grant (MR/S007849/1) and a Royal Society Grant (RGS\R1\191278) to JS. HG-C was supported by a Nigel Groome studentship from Oxford Brookes University. PA-C was supported by an EMBO Long Term Fellowship (ALTF-217-2018). JN was supported by funding from a BBSRC grant (BB/M011224/1) and the Osk. Huttunen Foundation (Doctoral grant)

Published
2020

Catalog

Books, media, physical & digital resources
See catalog results