1. A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome
- Author
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Chapman, Jarrod A, Mascher, Martin, Buluç, Aydın, Barry, Kerrie, Georganas, Evangelos, Session, Adam, Strnadova, Veronika, Jenkins, Jerry, Sehgal, Sunish, Oliker, Leonid, Schmutz, Jeremy, Yelick, Katherine A, Scholz, Uwe, Waugh, Robbie, Poland, Jesse A, Muehlbauer, Gary J, Stein, Nils, and Rokhsar, Daniel S
- Subjects
Biological Sciences ,Bioinformatics and Computational Biology ,Genetics ,Human Genome ,Bread ,Chromosome Mapping ,Contig Mapping ,DNA ,Complementary ,Genetic Linkage ,Genetic Variation ,Genome ,Plant ,Homozygote ,Nucleotides ,Polyploidy ,Reproducibility of Results ,Sequence Analysis ,DNA ,Triticum ,Environmental Sciences ,Information and Computing Sciences ,Bioinformatics - Abstract
Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive, de novo, a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat, Triticum aestivum, and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation and accuracy of our assembly is comparable or even exceeds that of a chromosome-by-chromosome shotgun assembly. Our assembly and mapping strategy uses only short read sequencing technology and is applicable to any species where it is possible to construct a mapping population.
- Published
- 2015