1. Molecular identification and DNA barcode screening of acaroid mites in ground flour dust
- Author
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Zhang, Wanyu, Cheng, Juan, Zhao, Yae, Niu, Dongling, and Guo, Hongsong
- Subjects
Flour -- Environmental aspects ,Mites -- Genetic aspects -- Environmental aspects -- Identification and classification ,DNA barcoding -- Methods ,Dust -- Environmental aspects ,Biological sciences - Abstract
Molecular identification of acaroid mites is difficult because of the scarcity of molecular data in GenBank. Here, acaroid mites collected from ground flour dust in Xi'an, China, were preliminarily morphologically classified/grouped. Universal primers were then designed to amplify and screen suitable DNA barcodes for identifying these mites. Sixty mite samples were morphologically classified into six groups. Groups 1-2 were identified to Dermatophagoides farinae and Tyrophagus putrescentiae, while Groups 3-6 were not identified to the species level. ITS2 exhibited higher efficiency in molecular identification in comparison with COI, 12S, and 16S. Groups 1-6 were identified as D. farinae, T. putrescentiae, Suidasia nesbitti, Chortoglyphus arcuatus, Lepidoglyphus destructor, and Gohieria sp., respectively. The phylogenetic results were consistent with the morphological classification. Group [+ or -] was further identified as G. fusca according to the morphology of the reproductive foramen. We conclude that the use of ITS2 and the availability of universal primers provide an ideal DNA barcode for molecular identification of acaroid mites. The use of multiple target genetic markers in conjunction with morphological approaches will improve the accuracy of Acaridida identification. Key words: acaroid mites, taxonomy, universal primers, molecular identification, DNA barcode. L'identification moleculaire des acariens est difficile en raison de la faible disponibilite de donnees moleculaires dans GenBank. Dans ce travail, des acariens echantillonnes dans la poussiere de farine a Xi'an en Chine ont ete classifies/groupes de maniere preliminaire sur la base de leur morphologie. Des amorces universelles ont ensuite ete produites pour amplifier et examiner differents codes-barres a ADN pour identifier ces acariens. Soixante echantillons ont ete classifies en six groupes sur la base de leur morphologie. Les groupes 1 et 2 comprenaient des echantillons appartenant au Dermatophagoides farinae et au Tyrophagus putrescentiae, tandis que les echantillons des groupes 3 a [+ or -] n'ont pas ete identifies au niveau de l'espece. L'ITS2 a montre une plus grande efficacite dans l'identification moleculaire que les sequences COI, 12S et 16S. Les groupes 1-6 ont ete identifies comme etant composes respectivement de D. farinae, T. putrescentiea, Suidasia nesbitti, Chrotoglyphus arcuatus, Lepidoglyphus destructor et Goheria sp. Les resultats d'une analyse phylogenetique etaient conformes a la classification morphologique. Le groupe [+ or -] a ensuite ete identifie comme etant compose du G. fusca sur la base de la morphologie du foramen reproducteur. Les auteurs concluent que l'utilisation de l'ITS2 et la disponibilite d'amorces universelles fournissent un code-barre ideal pour l'identification moleculaire des acariens. Le recours a plusieurs marqueurs genetiques, en conjonction avec les approches morphologiques, permettront d'ameliorer l'exactitude de l'identification des Acaridides. [Traduit par la Redaction] Mots-cles : acariens, taxonomie, amorces universelles, identification moleculaire, code-barre a ADN., Introduction Acaroid mites, belonging to the suborder Acaridida and the order Acariformes, are a large group of harmful mites that are prevalent in the human living environment where they pose [...]
- Published
- 2021
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