1. [Expression of the pro-opiomelanocortin gene in rats with inherited arterial hypertension].
- Author
-
Dashkevich VS, Pozdniakov SG, Markel' AL, and Mertvetsov NP
- Subjects
- Animals, Disease Models, Animal, Gene Expression physiology, Nucleic Acid Hybridization, Protein Biosynthesis genetics, Protein Sorting Signals genetics, RNA, Messenger genetics, Rats, Rats, Inbred Strains, Hypertension genetics, Pro-Opiomelanocortin genetics
- Abstract
Expression of pro-opiomelanocortin (POMC) gene in pituitary of rats from newly developed hypertensive strain (ISIAH strain) was studied by dot hybridization. The pUC8 plasmid containing 900 base pair (bp) segment or the human POMC gene corresponding to the major portion of the 3'-nontranslated mRNA region and 60 bp coding for the signal peptide, was used as a probe for hybridization. It was found that the expression of the POMC in pituitary of the hypertensive JSJAH rats was more than 3-fold gene lower as compared to normotensive Wistar strain. The latter is the original strain from which the ISIAH rats were bred. The mechanism of this phenomenon and its possible relation to the arterial hypertension are discussed.
- Published
- 1991