1. Correction: Mapping the genomic landscape of inherited retinal disease genes prioritizes genes prone to coding and noncoding copy-number variations
- Author
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Kristof Van Schil, Sarah Naessens, Stijn Van de Sompele, Marjolein Carron, Alexander Aslanidis, Caroline Van Cauwenbergh, Anja K. Mayer, Mattias Van Heetvelde, Miriam Bauwens, Hannah Verdin, Frauke Coppieters, Michael E. Greenberg, Marty G. Yang, Marcus Karlstetter, Thomas Langmann, Katleen De Preter, Susanne Kohl, Timothy J. Cherry, Bart P. Leroy, Elfride De Baere, James R Lupski, Claudia Carvalho, Max van Min, Petra Klous, Sarah De Jaegere, and Sally Hooghe
- Subjects
RNA, Untranslated ,DNA Copy Number Variations ,Genome, Human ,Correction ,Cadherin Related Proteins ,Chromosome Mapping ,Genetic mapping ,Genomics ,Sequence Analysis, DNA ,Regulatory Sequences, Nucleic Acid ,Cadherins ,Retinal diseases ,Open Reading Frames ,Databases, Genetic ,Humans ,Genetic Predisposition to Disease ,Genetic variation ,Eye Proteins ,Alleles ,Genetics (clinical) ,Genome-Wide Association Study ,Sequence Deletion - Abstract
PurposePart of the hidden genetic variation in heterogeneous genetic conditions such as inherited retinal diseases (IRDs) can be explained by copy-number variations (CNVs). Here, we explored the genomic landscape of IRD genes listed in RetNet to identify and prioritize those genes susceptible to CNV formation.MethodsRetNet genes underwent an assessment of genomic features and of CNV occurrence in the Database of Genomic Variants and literature. CNVs identified in an IRD cohort were characterized using targeted locus amplification (TLA) on extracted genomic DNA.ResultsExhaustive literature mining revealed 1,345 reported CNVs in 81 different IRD genes. Correlation analysis between rankings of genomic features and CNV occurrence demonstrated the strongest correlation between gene size and CNV occurrence of IRD genes. Moreover, we identified and delineated 30 new CNVs in IRD cases, 13 of which are novel and three of which affect noncoding, putative cis-regulatory regions. Finally, the breakpoints of six complex CNVs were determined using TLA in a hypothesis-neutral manner.ConclusionWe propose a ranking of CNV-prone IRD genes and demonstrate the efficacy of TLA for the characterization of CNVs on extracted DNA. Finally, this IRD-oriented CNV study can serve as a paradigm for other genetically heterogeneous Mendelian diseases with hidden genetic variation.
- Published
- 2019