1. Lack of clastogenic/genotoxic effects of Baccharis dracunculifolia extract on Swiss mouse peripheral blood cells
- Author
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F. F. Perazzo, N. S. Andrade, Edson Luis Maistro, Universidade Estadual Paulista (Unesp), Univ Jose Rosario Vellano, and Universidade Federal do Amapá (UNIFAP)
- Subjects
Male ,Erythrocytes ,Biology ,Asteraceae ,Single cell gel electrophoresis ,medicine.disease_cause ,Toxicology ,Clastogen ,Mice ,Baccharis dracunculifolia ,In vivo ,Micronucleus test ,Genetics ,medicine ,Animals ,Peripheral blood cell ,Molecular Biology ,Mutagenicity assay ,Micronucleus Tests ,Traditional medicine ,Dose-Response Relationship, Drug ,Plant Extracts ,food and beverages ,General Medicine ,Propolis ,biology.organism_classification ,Comet assay ,Female ,Comet Assay ,Genotoxicity ,DNA Damage - Abstract
Made available in DSpace on 2013-09-27T14:54:07Z (GMT). No. of bitstreams: 1 WOS000267218200032.pdf: 296807 bytes, checksum: 337a49e7688dc326d92b0bb1270102c4 (MD5) Previous issue date: 2008-01-01 Made available in DSpace on 2013-09-30T18:15:25Z (GMT). No. of bitstreams: 1 WOS000267218200032.pdf: 296807 bytes, checksum: 337a49e7688dc326d92b0bb1270102c4 (MD5) Previous issue date: 2008-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:31:44Z No. of bitstreams: 1 WOS000267218200032.pdf: 296807 bytes, checksum: 337a49e7688dc326d92b0bb1270102c4 (MD5) Made available in DSpace on 2014-05-20T13:31:44Z (GMT). No. of bitstreams: 1 WOS000267218200032.pdf: 296807 bytes, checksum: 337a49e7688dc326d92b0bb1270102c4 (MD5) Previous issue date: 2008-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Baccharis dracunculifolia de Candole (DC) ( Asteraceae) is indigenous throughout southeastern Latin America and is used by local people in traditional medicine. This plant is known to be the source of resin for the highly valued Brazilian green propolis. As no information is available on the safety of high doses of B. dracunculifolia extract, we evaluated the mutagenic potential of high doses of this plant extract in vivo on peripheral blood cells of Swiss mice using the comet assay and the micronucleus test. The extract was administered by gavage at doses of 1000, 1500 and 2000 mg/kg body weight. Peripheral blood cell samples were collected 4 and 24 h after treatment for the comet assay (genotoxicity assay), and at 48 and 72 h for the micronucleus test (clastogenicity assay). The B. dracunculifolia extract was devoid of clastogenic/genotoxic activity at all doses. Univ Estadual Paulista, Fac Filosofia & Ciencias, Dept Fonoaudiol, Marilia, SP, Brazil Univ Jose Rosario Vellano, Genet Lab, Alfenas, MG, Brazil Univ Fed Amapa, Lab Pesquisa Farmacos, Macapa, AP, Brazil Univ Estadual Paulista, Fac Filosofia & Ciencias, Dept Fonoaudiol, Marilia, SP, Brazil FAPESP: 06/57514-2 FAPEMIG: 1879/02 CNPq: 306544/2006-7
- Published
- 2009