1. RUNX3 gene polymorphisms and haplotypes in Mexican patients with colorectal cancer
- Author
-
María de la Luz Ayala-Madrigal, Melva Gutiérrez-Angulo, Nelly Margarita Macías-Gómez, M. Centeno-Flores, Jose Miguel Moreno-Ortiz, Jorge Peregrina-Sandoval, Cabrales E, Ramírez-Ramírez R, Suárez-Villanueva S, Muñiz-Mendoza R, and Maciel-Gutiérrez
- Subjects
Adult ,Male ,Linkage disequilibrium ,medicine.medical_specialty ,Single-nucleotide polymorphism ,Gastroenterology ,Polymorphism, Single Nucleotide ,Young Adult ,Polymorphism (computer science) ,Risk Factors ,Internal medicine ,Genotype ,Genetics ,medicine ,Odds Ratio ,Humans ,Genetic Predisposition to Disease ,Allele ,Molecular Biology ,Mexico ,Alleles ,Aged ,Aged, 80 and over ,business.industry ,Haplotype ,Case-control study ,General Medicine ,Odds ratio ,Middle Aged ,digestive system diseases ,Core Binding Factor Alpha 3 Subunit ,Haplotypes ,Case-Control Studies ,Female ,business ,Colorectal Neoplasms - Abstract
We analyzed a possible association between RUNX3 gene polymorphisms and haplotypes in Mexican patients with colorectal cancer (CRC). Genomic DNA samples were obtained from the peripheral blood of 176 Mexican patients with CRC at diagnosis and from 195 individuals that formed the control group. The polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism. Association was estimated by odds ratio (OR). The haplotypes and linkage disequilibrium were established using the Arlequin v3.5 software. We found that the RUNX3 polymorphisms analyzed were in Hardy-Weinberg equilibrium. The RUNX3 rs2236852 AA genotype and A allele showed association with CRC (OR = 0.39, 95%CI = 0.21-0.73, P < 0.01; OR = 0.65, 95%CI = 0.49-0.87, P < 0.01, respectively), while the rs6672420, rs11249206, and rs760805 polymorphisms did not show significant association with CRC. The TA haplotype (SNPs rs760805 and rs2236852) showed an increased risk for CRC (OR = 2.52, 95%CI = 1.47-4.30, P < 0.001). In conclusion, we found that the AA genotype and A allele of rs2236852 polymorphism confer a decreased CRC risk, while the TA haplotype appears to increase the risk of CRC development in Mexican patients.
- Published
- 2015