1. Cre recombinase specificity defined by thetau locus
- Author
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Lothar Lindemann, Gusztav Belteki, Andras Nagy, Nikolett Kabacs, Jody J. Haigh, Ella Korets-Smith, Caoyang Jiang, Kerry L. Tucker, and Marina Gertsenstein
- Subjects
Male ,Genetically modified mouse ,Green Fluorescent Proteins ,Cre recombinase ,Mice, Transgenic ,tau Proteins ,Locus (genetics) ,Biology ,Genome ,Mice ,Endocrinology ,Genetics ,Animals ,Humans ,Allele ,Crosses, Genetic ,Floxing ,Neurons ,Recombination, Genetic ,Mice, Inbred ICR ,Integrases ,Cell Biology ,Embryonic stem cell ,Molecular biology ,Gene Expression Regulation ,Organ Specificity ,Gene Targeting ,Female ,Cre-Lox recombination - Abstract
We generated a transgenic mouse line (tau::Cre) by targeting the Cre to the tau locus (Mapt). Based on previous reports on the expression of Tau during development, we expected the Cre recombinase to be expressed in a neuron-specific and pan-neuronal manner. However, intercrosses between the tau::Cre and the Cre-activatable reporter animals resulted in offspring with recombination either restricted to the nervous system or throughout the entire conceptus, indicating expression of Tau early in development. The percentage of neuron-specific excision was dependent on the Cre reporter used representing different Cre target sites in the mouse genome. In spite of the observed variability, our data suggest that the tau::Cre mouse line can be used for pan-neuronal recombination of floxed alleles when it is used with caution. genesis 40:131–138, 2004. © 2004 Wiley-Liss, Inc.
- Published
- 2004
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