1. Spatial and temporal expression pattern of sex-related genes in ovo-testis of the self-fertilizing mangrove killifish (Kryptolebias marmoratus).
- Author
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Qu M, Ding S, Schartl M, and Adolfi MC
- Subjects
- Animals, Female, Fish Proteins metabolism, Forkhead Transcription Factors metabolism, Gene Expression Profiling, Male, Microscopy, Electron, Transmission, Oogenesis genetics, Ovary metabolism, Ovary ultrastructure, Spatio-Temporal Analysis, Spermatogenesis genetics, Testis metabolism, Testis ultrastructure, Gene Expression Regulation, Developmental genetics, Killifishes physiology, Ovary growth & development, Self-Fertilization genetics, Testis growth & development
- Abstract
In vertebrates, sex determination and differentiation comprehend a fine balance between female and male factors, leading the bipotential anlage to develop towards ovary or testis, respectively. Nevertheless, the mangrove killifish, (Kryptolebias marmoratus) a simultaneous hermaphroditic species, could overcome those antagonistic pathways and evolved to develop and maintain reproductively active ovarian and testicular tissues in the same organ. Morphological and mRNA localization analyzes of developing and adult gonads demonstrate that genes related to testis (dmrt1 and amh) and ovary differentiation (foxl2 and sox9a) follow the same expression pattern observed in gonochoristic species, thus functioning as two independent organs. In addition, Amh expression patterns make it a strong candidate for initiation of the formation and maintenance of the testicular tissue in the hermaphroditic gonad. Differently from described so far, foxl3 seems to have an important role in oogenesis as well as spermatogenesis and gonadal structure., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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