1. Promoter polymorphisms of pri-miR-34b/c are associated with hepatocellular carcinoma
- Author
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Young Joo Jeon, Myung Su Son, Pil Won Park, Sung Pyo Hong, Won Hee Kim, Kyu Sung Rim, Chang-Il Kwon, Seong Gyu Hwang, Kwang Hyun Ko, Sung Won Kwon, Moon Ju Jang, and Nam Keun Kim
- Subjects
Male ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Genotyping Techniques ,Single-nucleotide polymorphism ,Kaplan-Meier Estimate ,Biology ,Bioinformatics ,medicine.disease_cause ,Polymerase Chain Reaction ,Polymorphism, Single Nucleotide ,Gastroenterology ,Asian People ,Gene Frequency ,Risk Factors ,Internal medicine ,microRNA ,Odds Ratio ,Genetics ,medicine ,Humans ,SNP ,Genetic Predisposition to Disease ,Allele ,Promoter Regions, Genetic ,neoplasms ,Alleles ,Genetic Association Studies ,Aged ,Neoplasm Staging ,Retrospective Studies ,Liver Neoplasms ,General Medicine ,Odds ratio ,Middle Aged ,medicine.disease ,digestive system diseases ,Confidence interval ,MicroRNAs ,Case-Control Studies ,Hepatocellular carcinoma ,Female ,Tumor Suppressor Protein p53 ,Carcinogenesis ,Polymorphism, Restriction Fragment Length - Abstract
Background Numerous studies have focused on the association between miR-34 family members, which are direct p53 targets, and carcinogenesis of many cancers, including hepatocellular carcinoma (HCC). This study aimed to assess whether polymorphisms in the single-nucleotide polymorphism miR-34b/c T>C (rs4938723) and TP53 Arg72Pro (rs1042522) increase the risk of HCC and influence outcome in patients with HCC. Patients and methods We enrolled 157 HCC patients and 201 cancer-free control subjects from the Korean population. MicroRNA polymorphisms were genotyped using the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method. Results We found that the miR-34b/c TC + CC frequency was significantly higher in HCC patients than in controls (adjusted odds ratio [AOR]: 1.580; 95% confidence interval [CI]: 1.029–2.426). The miR-34b/c CC-TP53 Arg/Arg combination significantly increased the risk of HCC (AOR: 13.644; 95% CI: 1.451–128.301). The SNPs miR-34b/c T>C and TP53 Arg72Pro(G>C) had no influence on survival of HCC patients. Conclusions Our findings suggest that loss of the T allele in miR-34b/c T>C, and the miR-34b/c CC-TP53 Arg/Arg combination increases the risk of HCC in the Korean population.
- Published
- 2013