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3 results on '"Karginov VA"'

1. Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes

Author

Mertvetsov Np, Donchenko Ap, Boris Illarionov, Protopopova Mv, Karginov Va, Blinov Vm, and J. I. Gitelson

Subjects
DNA, Bacterial, Molecular Sequence Data, Restriction Mapping, medicine.disease_cause, law.invention, chemistry.chemical_compound, Photobacterium leiognathi, Bacterial Proteins, law, Operon, Genetics, medicine, Bioluminescence, Luciferase, Amino Acid Sequence, Cloning, Molecular, Luciferases, Gene, Escherichia coli, biology, Base Sequence, Vibrio harveyi, Photobacterium, General Medicine, biology.organism_classification, Molecular biology, chemistry, Genes, Bacterial, Luminescent Measurements, Recombinant DNA, DNA, Acyltransferases
Abstract

Genes encoding luminescence of Photobacterium leiognathi have been cloned in Escherichia coli. The luminescent clones were readily apparent. Among them, a clone containing a recombinant plasmid with a 13.5-kb insertion was identified. This DNA fragment contained all of the luminescence-encoding genes. The luciferase-encoding genes (lux) in this DNA fragment were localized. We have sequenced a part of the cloned lux region and identified the luxA, luxB and luxG genes encoding the alpha and beta subunits of luciferase and a gamma protein with an Mr of 26,180, respectively. The analysis of deduced amino acid sequences and comparison with known luciferase sequences from Vibrio harveyi, indicate the common origin of these proteins.

Published
1990

2. Isolation of bioluminescent functions from Photobacterium leiognathi: analysis of luxA, luxB, luxG and neighboring genes.

Author

Illarionov BA, Blinov VM, Donchenko AP, Protopopova MV, Karginov VA, Mertvetsov NP, and Gitelson JI

Subjects
Acyltransferases genetics, Amino Acid Sequence, Bacterial Proteins genetics, Base Sequence, Cloning, Molecular, DNA, Bacterial genetics, Molecular Sequence Data, Operon, Restriction Mapping, Genes, Bacterial, Luciferases genetics, Luminescent Measurements, Photobacterium genetics
Abstract

Genes encoding luminescence of Photobacterium leiognathi have been cloned in Escherichia coli. The luminescent clones were readily apparent. Among them, a clone containing a recombinant plasmid with a 13.5-kb insertion was identified. This DNA fragment contained all of the luminescence-encoding genes. The luciferase-encoding genes (lux) in this DNA fragment were localized. We have sequenced a part of the cloned lux region and identified the luxA, luxB and luxG genes encoding the alpha and beta subunits of luciferase and a gamma protein with an Mr of 26,180, respectively. The analysis of deduced amino acid sequences and comparison with known luciferase sequences from Vibrio harveyi, indicate the common origin of these proteins.

Published
1990
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3. Molecular cloning of a bovine immunoglobulin lambda chain cDNA.

Author

Ivanov VN, Karginov VA, Morozov IV, and Gorodetsky SI

Subjects
Amino Acid Sequence, Animals, Base Sequence, Cattle, DNA Restriction Enzymes, Female, Mammary Glands, Animal immunology, Molecular Sequence Data, Nucleic Acid Hybridization, Plasmids, Protein Biosynthesis, RNA, Messenger genetics, Cloning, Molecular, DNA genetics, Genes, Immunoglobulin, Immunoglobulin lambda-Chains genetics
Abstract

A cDNA library of the bovine mammary gland constructed in pBR322 was screened by mRNA hybrid-selected translation and by differential hybridization. Several immunoglobulin (Ig) lambda light-chain clones were identified and sequenced. Nucleotide sequence comparison of bovine and human Ig lambda chains showed a high degree of homology for constant regions and for J regions. The amino acid (aa) sequence encoded by the constant region of the bovine Ig lambda chain cDNA contains 107 aa with differences at 24 aa positions from the human Ig lambda chain. Three complementarity-determining regions (CDR1,2,3) characteristic of the variable region of bovine Ig lambda chain cDNA can be distinguished. The bovine and human sequences display good homology in the framework region 3 (FR3) but only patches of homology throughout the FR2 region. The 5' end of the bovine Ig lambda chain cDNA fragment of clone 1-14E contains five stop codons: two in CDR1, one in FR1 and two in the hydrophobic prepeptide region. These data suggest that the Ig lambda mRNA of clone 1-14E is transcribed from the V lambda pseudogene.

Published
1988
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