1. Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabA34) specific for human plasma apolipoprotein A-I
- Author
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Byung-Kwon Choi, Moon Hi Han, Yong-Bok Park, Dong Ik Lee, Ju-Won Kwak, Won-Kyung Cho, and Sang Han Lee
- Subjects
DNA, Complementary ,Apolipoprotein B ,medicine.drug_class ,Molecular Sequence Data ,Monoclonal antibody ,Immunoglobulin light chain ,law.invention ,Immunoglobulin Fab Fragments ,Mice ,law ,Complementary DNA ,Genetics ,medicine ,Animals ,Humans ,Nucleotide ,Amino Acid Sequence ,Cloning, Molecular ,Gene ,chemistry.chemical_classification ,Mice, Inbred BALB C ,Hybridomas ,biology ,Apolipoprotein A-I ,Base Sequence ,Antibodies, Monoclonal ,General Medicine ,Molecular biology ,Amino acid ,chemistry ,biology.protein ,Recombinant DNA ,Immunoglobulin Light Chains ,Immunoglobulin Heavy Chains - Abstract
We have determined the nucleotide (nt) sequences encoding the heavy (H)- and light (L)-chains of the Fab fragment of a murine monoclonal antibody, MabA34 (gamma1, kappa), which is specific for human plasma apolipoprotein A-I of high-density lipoproteins. The variable (V) regions of the H- and L-chains were revealed to be members of mouse H-chain subgroup II(A) and kappa L-chain subgroup II, respectively. A few unusual amino acids in the V region of the H-chain, and nt residues probably introduced by somatic mutations from germline genes were also identified.
- Published
- 1996