1. Pros and cons of pDNA and mRNA transfection to study mRNA translation in mammalian cells.
- Author
-
Andreev, Dmitry E., Terenin, Ilya M., Dmitriev, Sergey E., and Shatsky, Ivan N.
- Subjects
- *
MESSENGER RNA , *GENE transfection , *PROTEIN synthesis , *STRINGENT control (Bacteria) , *GENETIC regulation , *GENETICS of autoimmune diseases - Abstract
Protein synthesis in eukaryotes is subject to stringent control. The misregulation of translation of certain mRNAs is often a hallmark of many diseases, including malignancies and autoimmune disorders. To understand why and how it happens, it is important to investigate the translational control of specific mRNAs. In this case, one could use reporter mRNAs in order to identify cis -acting elements responsible for regulation. Here we overview plasmid DNA (pDNA) and mRNA transfections, their pitfalls and limitations, as well as some emerging applications for mRNA transfection. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF