1. Genome-wide identification, classification, expression profiling and DNA methylation (5mC) analysis of stress-responsive ZFP transcription factors in rice (Oryza sativa L.).
- Author
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Ahmad, Fiaz, Farman, Kiran, Waseem, Muhammad, Rana, Rashid Mehmood, Nawaz, Muhammad Amjad, Rehman, Hafiz Mamoon, Abbas, Tanveer, Baloch, Faheem Shehzad, Akrem, Ahmed, Huang, Ji, and Zhang, Hongsheng
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EPIGENOMICS , *RICE , *DNA methylation , *DNA fingerprinting , *TRANSCRIPTION factors , *GENE expression in plants , *DROUGHT tolerance - Abstract
Cytosine DNA methylation (5mC) is an epigenetic mark that regulates gene expression in plant responses to environmental stresses. Zinc-finger protein (ZFP) is the largest family of DNA-binding transcription factors that also plays an essential role in eukaryote. In plant we have already identified and characterized different useful ZFP-genes. While, the main objective of this research was to observe and identify more targeted stress responsive genes of ZFPs epigenetically throughout genome in rice for the first time. A comprehensive correlation analysis was performed through methylated DNA immunoprecipitation (MeDIP)-chip hybridization in rice under salt and osmotic stresses. High salinity and drought are two major abiotic hazards that are destroying the crop world-wide. As a result, Through-out genome 14 unique stress responsive transcription factors of ZFP-genes with varying level of methylation and expression under two conditions (control vs. stress) were isolated. All the identified genes were confirmed from different databases for their specific structure, cis-regulatory elements, phylogenetic analysis, and synteny analysis. Moreover, the tissue-specific expression patterns, and expression under abiotic and phytohormones stresses were also investigated. Phylogenetically all the genes were divided into 6 distinct subgroups with Arabidopsis and orthologous proteins were find-out through synteny analysis. Available RNA-seq data in response to various phytohormones provided hormone inducible gene expression profile. Through Reverse Transcriptase qPCR (RT-qPCR) analysis tissue-specific expression in shoot and root over various time points against salt and osmotic stresses exhibited the diverse expression patterns of identified genes. Overall, the present study providing a foundation for in-depth characterization of identified genes and to further understand the epigenetic role of DNA methylation for genes expression and environmental stresses regulation in higher plant. • 14 unique OsZFP genes partially or fully methylated were identified through MeDIP-chip. • All OsZFPs were clustered in three clades. • RNA-seq data in response to various phytohormones provided hormone specific genes expression profiles. • The candidate rice ZFP genes exhibited diverse expression patterns under salinity and drought stresses. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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