1. Methylation-associated silencing of microRNA-34b in hepatocellular carcinoma cancer.
- Author
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Xie, Kaipeng, Liu, Jibin, Chen, Jiaping, Dong, Jing, Ma, Hongxia, Liu, Yao, and Hu, Zhibin
- Subjects
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LIVER cancer , *METHYLATION , *MICRORNA genetics , *GENE silencing , *ONCOGENES , *CARCINOGENESIS , *GENETICS - Abstract
Abstract: MicroRNAs (miRNAs) can act as oncogenes or tumor-suppressor genes in human cancers including HCC. Previous studies have identified miR-34 family as an important component of the tumor suppressor network during carcinogenesis. In this study, we investigated the methylation status of miR-34 family in HCC tumor and adjacent non-tumor tissues using methylation-specific PCR (MSP). The methylation frequencies of miR-34a and miR-34b/c were 72.1% (31/43) and 79.1% (34/43) in HCC tissues, which were significantly higher than that in the adjacent non-tumor tissues (P <0.05), respectively. The results were validated by bisulfite sequencing PCR (BSP). Quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis showed that the expression of miR-34a and miR-34b was significantly down-regulated in HCC tissues compared with adjacent non-tumor tissues (P <0.05). Moreover, the expression of miR-34b was inversely correlated to CpG island methylation in tumor tissues, but not for miR-34a. In summary, our results suggest that DNA methylation may be involved in the inactivation of miR-34b in HCC. [Copyright &y& Elsevier]
- Published
- 2014
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