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3 results on '"Matthew O. Wiens"'

1. Whole blood genome-wide transcriptome profiling and metagenomics next-generation sequencing in young infants with suspected sepsis in a low-and middle-income country: A study protocol [version 2; peer review: 2 approved]

Author

Constantin R. Popescu, Bentry Tembo, Rhoda Chifisi, Miranda M.M. Cavanagh, Amy Huei-Yi Lee, Blessings Chiluzi, Emily J. Ciccone, Gerald Tegha, Esther Alonso-Prieto, Jennifer Claydon, Dustin Dunsmuir, Mike Irvine, Guy Dumont, J. Mark Ansermino, Matthew O. Wiens, Jonathan J. Juliano, Niranjan Kissoon, Tisungane Mvalo, Norman Lufesi, Msandeni Chiume-Kayuni, and Pascal M. Lavoie

Subjects
Medicine
Abstract

Conducting collaborative and comprehensive epidemiological research on neonatal sepsis in low- and middle-income countries (LMICs) is challenging due to a lack of diagnostic tests. This prospective study protocol aims to obtain epidemiological data on bacterial sepsis in newborns and young infants at Kamuzu Central Hospital in Lilongwe, Malawi. The main goal is to determine if the use of whole blood transcriptome host immune response signatures can help in the identification of infants who have sepsis of bacterial causes. The protocol includes a detailed clinical assessment with vital sign measurements, strict aseptic blood culture protocol with state-of-the-art microbial analyses and RNA-sequencing and metagenomics evaluations of host responses and pathogens, respectively. We also discuss the directions of a brief analysis plan for RNA sequencing data. This study will provide robust epidemiological data for sepsis in neonates and young infants in a setting where sepsis confers an inordinate burden of disease.

Published
2020
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2. Whole blood genome-wide transcriptome profiling and metagenomics next-generation sequencing in young infants with suspected sepsis in a low-and middle-income country: A study protocol

Author

Pascal M. Lavoie, Jonathan J. Juliano, Bentry Tembo, Dustin Dunsmuir, Emily J Ciccone, Jennifer Claydon, Matthew O. Wiens, Michael A. Irvine, Gerald Tegha, Rhoda Chifisi, Constantin R. Popescu, Norman Lufesi, Guy A. Dumont, Miranda M.M. Cavanagh, Niranjan Kissoon, Msandeni Chiume-Kayuni, Amy S. Lee, Esther Alonso-Prieto, Tisungane Mvalo, J. Mark Ansermino, and Blessings Chiluzi

Subjects
0301 basic medicine, medicine.medical_specialty, Neonatal sepsis, medicine.diagnostic_test, business.industry, Health Policy, Public Health, Environmental and Occupational Health, Medicine (miscellaneous), medicine.disease, Biochemistry, Genetics and Molecular Biology (miscellaneous), DNA sequencing, Transcriptome, Sepsis, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Immunology and Microbiology (miscellaneous), Metagenomics, Epidemiology, medicine, Blood culture, Intensive care medicine, business, Prospective cohort study, 030217 neurology & neurosurgery
Abstract

Conducting collaborative and comprehensive epidemiological research on neonatal sepsis in low- and middle-income countries (LMICs) is challenging due to a lack of diagnostic tests. This prospective study protocol aims to obtain epidemiological data on bacterial sepsis in newborns and young infants at Kamuzu Central Hospital in Lilongwe, Malawi. The main goal is to determine if the use of whole blood transcriptome host immune response signatures can help in the identification of infants who have sepsis of bacterial causes. The protocol includes a detailed clinical assessment with vital sign measurements, strict aseptic blood culture protocol with state-of-the-art microbial analyses and RNA-sequencing and metagenomics evaluations of host responses and pathogens, respectively. We also discuss the directions of a brief analysis plan for RNA sequencing data. This study will provide robust epidemiological data for sepsis in neonates and young infants in a setting where sepsis confers an inordinate burden of disease.

Published
2020

3. Whole blood genome-wide transcriptome profiling and metagenomics next-generation sequencing in young infants with suspected sepsis in low-and middle-income countries: A study protocol

Author

Constantin R. Popescu, Bentry Tembo, Rhoda Chifisi, Miranda M.M. Cavanagh, Amy Huei-Yi Lee, Blessings Chiluzi, Emily J. Ciccone, Gerald Tegha, Esther Alonso-Prieto, Jennifer Claydon, Dustin Dunsmuir, Mike Irvine, Guy Dumont, J. Mark Ansermino, Matthew O. Wiens, Jonathan J. Juliano, Niranjan Kissoon, Tisungane Mvalo, Norman Lufesi, Msandeni Chiume-Kayuni, and Pascal M. Lavoie

Subjects
0301 basic medicine, metagenomics, diagnosis, Health Policy, Public Health, Environmental and Occupational Health, Medicine (miscellaneous), RNA sequencing, Articles, low- and middle-income country, Biochemistry, Genetics and Molecular Biology (miscellaneous), blood culture, sepsis, Study Protocol, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Immunology and Microbiology (miscellaneous), young infants, epidemiology, 030217 neurology & neurosurgery, Newborns, prospective study
Abstract

Conducting collaborative and comprehensive epidemiological research on neonatal sepsis in low- and middle-income countries (LMICs) is challenging due to a lack of diagnostic tests. This prospective study protocol aims to obtain epidemiological data on bacterial sepsis in newborns and young infants at Kamuzu Central Hospital in Lilongwe, Malawi. The main goal is to determine if the use of whole blood transcriptome host immune response signatures can help in the identification of infants who have sepsis of bacterial causes. The protocol includes a detailed clinical assessment with vital sign measurements, strict aseptic blood culture protocol with state-of-the-art microbial analyses and RNA-sequencing and metagenomics evaluations of host responses and pathogens, respectively. We also discuss the directions of a brief analysis plan for RNA sequencing data. This study will provide robust epidemiological data for sepsis in neonates and young infants in a setting where sepsis confers an inordinate burden of disease.

Published
2020

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