1. Transposon-tagging identifies novel pathogenicity genes in Fusarium graminearum
- Author
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Taiguo Liu, Gert H. J. Kema, Cees Waalwijk, Sarrah Ben M’Barek, Xu Zhang, Marie-Josée Daboussi, Theo van der Lee, Marie Dufresne, Xiude Xu, Wenwei Zhang, Institut de génétique et microbiologie [Orsay] (IGM), and Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,Transposable element ,family ,oxysporum ,Transposon tagging ,MESH: Triticum ,arabidopsis-thaliana ,MESH: Virulence ,Biology ,01 natural sciences ,Microbiology ,Genome ,Insertional mutagenesis ,03 medical and health sciences ,MESH: Plant Diseases ,Fusarium ,mediated transformation ,Genetics ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Gene ,Triticum ,Transposase ,Plant Diseases ,030304 developmental biology ,MESH: Fusarium ,MESH: Genetic Complementation Test ,0303 health sciences ,Biointeracties and Plant Health ,Virulence ,magnaporthe-grisea ,Genetic Complementation Test ,filamentous fungi ,food and beverages ,Sleeping Beauty transposon system ,inverted-repeat elements ,Laboratorium voor Phytopathologie ,Mutagenesis, Insertional ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,MESH: DNA Transposable Elements ,MESH: Mutagenesis, Insertional ,Laboratory of Phytopathology ,DNA Transposable Elements ,insertional mutagenesis ,rice blast fungus ,PRI Biointeractions en Plantgezondheid ,Transposon mutagenesis ,genome-wide analysis ,010606 plant biology & botany - Abstract
International audience; With the increase of sequenced fungal genomes, high-throughput methods for functional analyses of genes are needed. We assessed the potential of a new transposon mutagenesis tool deploying a Fusarium oxysporum miniature inverted-repeat transposable element mimp1, mobilized by the transposase of impala, a Tc1-like transposon, to obtain knock-out mutants in Fusarium graminearum. We localized 91 mimp1 insertions which showed good distribution over the entire genome. The main exception was a major hotspot on chromosome 2 where independent insertions occurred at exactly the same nucleotide position. Furthermore insertions in promoter regions were over-represented. Screening 331 mutants for sexual development, radial growth and pathogenicity on wheat resulted in 19 mutants (5.7%) with altered phenotypes. Complementation with the original gene restored the wild-type phenotype in two selected mutants demonstrating the high tagging efficiency. This is the first report of a MITE transposon tagging system as an efficient mutagenesis tool in F. graminearum.
- Published
- 2008
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