1. Purification of a new fungal mannose-specific lectin from Penicillium chrysogenum and its aphicidal properties
- Author
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Eric Haubruge, Frédéric Colinet, Frédéric Francis, Karimi Jaber, and Daniel Portetelle
- Subjects
Insecticides ,Mushroom ,Hemagglutination ,Lectin ,Hemagglutination Tests ,Penicillium chrysogenum ,Biology ,biology.organism_classification ,Microbiology ,Fungal Proteins ,Mannose-Binding Lectins ,Infectious Diseases ,Mannose-Specific Lectin ,Biochemistry ,Concanavalin A ,Aphids ,Genetics ,biology.protein ,Animals ,Rabbits ,Myzus persicae ,Chromatography column ,Ecology, Evolution, Behavior and Systematics - Abstract
Several Ascomycetes fungi are commonly used in bio-industries and provide available industrial residues for lectin extraction to be valuable. A lectin from Penicillium chrysogenum, named PeCL, was purified from a fungal culture using gel-filtration chromatography column. PeCL was found to be a mannose-specific lectin by haemagglutination activity towards rabbit erythrocyte cells and was visualised on SDS-PAGE gel. Purified PeCL fraction was delivered via artificial diet to Myzus persicae aphid and was demonstrated to be aphicidal at 0.1 % with higher toxic efficiency than a known mannose-binding lectin Concanavalin A (ConA). A fast and efficient way to purify PeCL and a potential use in pest control is described.
- Published
- 2011
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