This paper shows the results of studying the varieties of blackberry ‘Darrow’, ‘Thornfree’, ‘Smoothstem’ to obtain organic production in the climatic conditions of the Republic of Moldova. The research was based on monitoring the phenological, organoleptic, and biometric indicators in 2011, 2013 and 2014. The use of varieties with increased resistance to environmental conditions, pests and diseases is one of the essential ingredients, originated from the organic farming. During the years 2013-2014 in all varieties studied was not observed frostbites on blackberry plants, no symptoms of disease and pest attack. Biochemical composition of fruits was determined to remark the nutritional qualities and taste of organic blackberry fruit. In assessing the coefficient sugar / acidity, best manifested variety was ‘Thornfree’ by the average amount of 2.87, followed by ’Smoothstem’ variety - 2.76. After the dry matter content, stands out the Smoothstem variety, by the average amount of 12.02% for the period studied, but by the content of vitamin C is predominant the variety ‘Darrow’ with thorns by the average amount of 26.13 mg%. Regarding the level of productivity, the highest yields in 2014 were obtained from varieties without thorns, especially the variety ‘Thornfree’ with a value of 7.64 kg in a bush.