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Continuous cropping of soybean leads to soil environment deterioration and soil-borne disease exacerbation, which in turn limits the sustainability of agricultural production. Chitin amendments are considered promising methods for alleviating soybean continuous cropping obstacles; however, the underlying mechanisms of soil sickness reduction remain unclear. In this study, soil amendments with pure and crude chitin at different addition dosages were employed to treat diseased soil induced by continuous cropping of soybean for five years. Chitin amendments, especially crude chitin, remarkably increased soil pH, available phosphorus (AP), potassium (AK) and nitrate nitrogen ( NO3−-N) contents, and improved soybean plant growth and soil microbial activities (FDA). Additionally, chitin application significantly enriched the relative abundances of the potential biocontrol bacteria Sphingomonas, Streptomyces, and Bacillus and the fungi Mortierella, Purpureocillium, and Metarhizium while depleted those of the potential plant pathogens Fusarium, Cylindrocarpon and Paraphoma. Moreover, chitin amendments induced looser pathogenic subnetwork structures and less pathogenic cooperation with other connected microbial taxa in the rhizosphere soils. The structural equation model (SEM) revealed that pure and crude chitin amendments promoted soybean plant growth by indirectly regulating soil pH-mediated soil microbial activities and potentially beneficial microbes, respectively. Therefore, the reduction strategies for continuous cropping obstacles by adding pure and crude chitin were distinct; pure chitin amendments showed general disease suppression, while crude chitin exhibited specific disease suppression. Overall, chitin amendments could suppress potential plant pathogens and improve soil health, thereby promoting soybean growth, which provides new prospects for cultivation practices to control soybean continuous cropping obstacles.

Crop photosynthetic capacity in response to climate change likely constrains crop productivity and adaptability to changing environments, which requests the investigation on the dynamics of photosynthetic parameters over growth season among varieties, especially in cold-temperate regions. Three Japonica rice cultivars i.e., Shoubaimao (SH), Hejiang 19 (HJ); Longjing 31, (LJ). were planted under the control, e[CO2] (700 μmol mol-1), warming (2°C above the air temperature) and the co-elevation of [CO2] and temperature in open-top chambers (OTC). The objective of this study is to examine the rice photosynthetic parameters, water use efficiency (WUE) and yield formation in responses to the co-elevation of [CO2] and temperature which is the main predicted features of future climate. e[CO2] significantly increased An of SH, HJ and LJ by 37%, 39% and 23% in comparison to 34%, 34% and 27% under elevated [CO2] plus warming, respectively. However, An had a weak response to warming for three cultivars. [CO2] and temperature co-elevation significantly decreased the stomatal conductance, resulting in a significant increase of the WUE. e[CO2] significantly increased Vc, max, Jmax and Jmax/Vc, max. e[CO2] significantly increased grain yield and grain number of all cultivars. The positive effect of co-elevation of [CO2] and temperature on grain yield was less than e[CO2]. Warming is likely to partially offset the increased photosynthetic rate caused by e[CO2]. The [CO2] and temperature co-elevation may be favorable to rice crop with increasing the photosynthetic ability of rice crop and improving water use efficiency. The present study provided evidence that the rice genotypic difference in photosynthetic potential under [CO2] and temperature co-elevation. Therefore, it is crucial to explore a broader range of phenotypes and cultivars to be applied to climate change response research, advancing the knowledge that climate change impacts rice crop under the cold-temperate climate region.

Although the effect of elevated CO2 (eCO2) on soybean yield has been well documented, few studies have addressed seed quality, particularly at the fresh edible (R6) and mature stages (R8). Under the current global scenario of increasing CO2 levels, this potentially threatens the nutritional content and quality of food crops. Using four soybean cultivars, we assessed the effects of eCO2 on the concentrations of crude protein, crude oil, and isoflavones and analyzed the changes in free amino acids, fatty acids, and mineral elements in seeds. At R6, eCO2 had no influence on soybean seed protein and oil concentrations. At R8, eCO2 significantly decreased seed protein concentration but increased seed oil concentration; it also significantly decreased total free amino acid concentration. However, at the same stage, the proportion of oleic acid (18:1) among fatty acids increased in response to eCO2 in the cultivars of Zhongke-maodou 2 (ZK-2) and Zhongke-maodou 3 (ZK-3), and a similar trend was found for linoleic acid (18:2) in Zhongke-maodou 1 (ZK-1) and Hei-maodou (HD). Total isoflavone concentrations increased significantly at both the R6 and R8 stages in response to eCO2. Compared with ambient CO2, the concentrations of K, Ca, Mg, P, and S increased significantly under eCO2 at R6, while the Fe concentration decreased significantly. The response of Zn and Mn concentrations to eCO2 varied among cultivars. At R8 and under eCO2, Mg, S, and Ca concentrations increased significantly, while Zn and Fe concentrations decreased significantly. These findings suggest that eCO2 is likely to benefit from the accumulation of seed fat and isoflavone but not from that of protein. In this study, the response of seed mineral nutrients to eCO2 varied between cultivars.

Nitrogen deficiency limits crop performance under elevated CO2 (eCO2), depending on the ability of plant N uptake. However, the dynamics and redistribution of N2 fixation, and fertilizer and soil N use in legumes under eCO2 have been little studied. Such an investigation is essential to improve the adaptability of legumes to climate change. We took advantage of genotype-specific responses of soybean to increased CO2 to test which N-uptake phenotypes are most strongly related to enhanced yield. Eight soybean cultivars were grown in open-top chambers with either 390 ppm (aCO2) or 550 ppm CO2 (eCO2). The plants were supplied with 100 mg N kg−1 soil as 15N-labeled calcium nitrate, and harvested at the initial seed-filling (R5) and full-mature (R8) stages. Increased yield in response to eCO2 correlated highly (r = 0.95) with an increase in symbiotically fixed N during the R5 to R8 stage. In contrast, eCO2 only led to small increases in the uptake of fertilizer-derived and soil-derived N during R5 to R8, and these increases did not correlate with enhanced yield. Elevated CO2 also decreased the proportion of seed N redistributed from shoot to seeds, and this decrease strongly correlated with increased yield. Moreover, the total N uptake was associated with increases in fixed-N per nodule in response to eCO2, but not with changes in nodule biomass, nodule density, or root length.

Crop photosynthetic capacity in response to climate change likely constrains crop productivity and adaptability to changing environments, which requests the investigation on the dynamics of photosynthetic parameters over growth season among varieties, especially in cold-temperate regions. Three Japonica rice cultivars i.e., Shoubaimao (SH), Hejiang 19 (HJ); Longjing 31, (LJ). were planted under the control, e[CO2] (700 μmol mol-1), warming (2°C above the air temperature) and the co-elevation of [CO2] and temperature in open-top chambers (OTC). The objective of this study is to examine the rice photosynthetic parameters, water use efficiency (WUE) and yield formation in responses to the co-elevation of [CO2] and temperature which is the main predicted features of future climate. e[CO2] significantly increased An of SH, HJ and LJ by 37%, 39% and 23% in comparison to 34%, 34% and 27% under elevated [CO2] plus warming, respectively. However, An had a weak response to warming for three cultivars. [CO2] and temperature co-elevation significantly decreased the stomatal conductance, resulting in a significant increase of the WUE. e[CO2] significantly increased Vc, max, Jmax and Jmax/Vc, max. e[CO2] significantly increased grain yield and grain number of all cultivars. The positive effect of co-elevation of [CO2] and temperature on grain yield was less than e[CO2]. Warming is likely to partially offset the increased photosynthetic rate caused by e[CO2]. The [CO2] and temperature coelevation may be favorable to rice crop with increasing the photosynthetic ability of rice crop and improving water use efficiency. The present study provided evidence that the rice genotypic difference in photosynthetic potential under [CO2] and temperature co-elevation. Therefore, it is crucial to explore a broader range of phenotypes and cultivars to be applied to climate change response research, advancing the knowledge that climate change impacts rice crop under the cold-temperate climate region. [ABSTRACT FROM AUTHOR]

Nitrogen deficiency limits crop performance under elevated CO2 (eCO2), depending on the ability of plant N uptake. However, the dynamics and redistribution of N2 fixation, and fertilizer and soil N use in legumes under eCO2 have been little studied. Such an investigation is essential to improve the adaptability of legumes to climate change. We took advantage of genotype-specific responses of soybean to increased CO2 to test which N-uptake phenotypes are most strongly related to enhanced yield. Eight soybean cultivars were grown in open-top chambers with either 390 ppm (aCO2) or 550 ppm CO2 (eCO2). The plants were supplied with 100mg N kg-1 soil as 15N-labeled calcium nitrate, and harvested at the initial seed-filling (R5) and full-mature (R8) stages. Increased yield in response to eCO2 correlated highly (r = 0.95) with an increase in symbiotically fixed N during the R5 to R8 stage. In contrast, eCO2 only led to small increases in the uptake of fertilizer-derived and soil-derived N during R5 to R8, and these increases did not correlate with enhanced yield. Elevated CO2 also decreased the proportion of seed N redistributed from shoot to seeds, and this decrease strongly correlated with increased yield. Moreover, the total N uptake was associated with increases in fixed-N per nodule in response to eCO2, but not with changes in nodule biomass, nodule density, or root length. [ABSTRACT FROM AUTHOR]