1. Peptides as Quorum Sensing Molecules: Measurement Techniques and Obtained Levels In vitro and In vivo
- Author
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Nathan Debunne, Frederick Verbeke, Christophe Van de Wiele, Evelien Wynendaele, Yorick Janssens, Bart De Spiegeleer, and Severine De Craemer
- Subjects
0301 basic medicine ,Gram-negative bacteria ,quorum sensing peptides ,030106 microbiology ,GUT-BRAIN AXIS ,Homoserine ,microbiome ,TETRACYCLINE RESISTANCE PLASMID ,NEUROENDOCRINE STRESS HORMONES ,03 medical and health sciences ,chemistry.chemical_compound ,In vivo ,N-3-OXODODECANOYL HOMOSERINE LACTONE ,PSEUDOMONAS-AERUGINOSA AUTOINDUCER ,N-ACYLHOMOSERINE LACTONES ,GRAM-NEGATIVE BACTERIA ,biology ,reporter bacteria ,General Neuroscience ,Biology and Life Sciences ,CHROMATOGRAPHY-MASS-SPECTROMETRY ,food and beverages ,SEX-PHEROMONE INHIBITOR ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,biosensors ,In vitro ,Quorum sensing ,analytical methods ,030104 developmental biology ,Biochemistry ,chemistry ,chromatography ,Autoinducer ,Biosensor ,Bacteria ,THIN-LAYER-CHROMATOGRAPHY ,Neuroscience - Abstract
The expression of certain bacterial genes is regulated in a cell-density dependent way, a phenomenon called quorum sensing. Both Gram-negative and Gram-positive bacteria use this type of communication, though the signal molecules (auto-inducers) used by them differ between both groups: Gram-negative bacteria use predominantly N-acyl homoserine lacton (AHL) molecules (autoinducer-1, AI-1) while Gram-positive bacteria use mainly peptides (autoinducer peptides, AIP or quorum sensing peptides). These quorum sensing molecules are not only involved in the inter-microbial communication, but can also possibly cross-talk directly or indirectly with their host. This review summarizes the currently applied analytical approaches for quorum sensing identification and quantification with additionally summarizing the experimentally found in vivo concentrations of these molecules in humans.
- Published
- 2017
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