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Start Over Author "Almeida, Ana" Journal frontiers in microbiology
18 results on '"Almeida, Ana"'

1. Paracoccidioides spp.: the structural characterization of extracellular matrix, expression of glucan synthesis and associated genes and adhesins during biofilm formation

Author

Oliveira, Lariane Teodoro, primary, Marcos, Caroline Maria, additional, Cabral, Ana Karla Lima Freire, additional, Medina-Alarcón, Kaila Petronila, additional, Pires, Regina Helena, additional, Fusco-Almeida, Ana Marisa, additional, and Mendes-Giannini, Maria José Soares, additional

Published
2024
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2. Paracoccidioides spp.: the structural characterization of extracellular matrix, expression of glucan synthesis and associated genes and adhesins during biofilm formation.

Author

Teodoro Oliveira, Lariane, Maria Marcos, Caroline, Lima Freire Cabral, Ana Karla, Petronila Medina-Alarcón, Kaila, Helena Pires, Regina, Marisa Fusco-Almeida, Ana, and Soares Mendes-Giannini, Maria Jose

Subjects
GENE expression, EXTRACELLULAR matrix, BIOFILMS, GLUCANS, BETA-glucans, ANTISENSE DNA, PARACOCCIDIOIDES brasiliensis, GENTIAN violet
Abstract

The genus Paracoccidioides includes Paracoccidioides lutzii and the Paracoccidioides brasiliensis complex, which comprises four phylogenetic species. A key feature distinguishing planktonic growth from biofilm is the presence of a 3D extracellular matrix (ECM). Therefore, in this study, we analyzed biofilm formation in dierent species of Paracoccidioides yeast phase, characterized the structural elements of the matrix of P. brasiliensis (Pb18), P. lutzii (Pl01 and 8334) and P. restrepiensis (339 and 192) and evaluated the expression of glucan genes, according to the stage of biofilm evolution for P. brasiliensis. The strains were cultivated in planktonic and biofilm form for 24–144 h. The fungi biomass and metabolic activity were determined by crystal violet and tetrazolium salt reduction (XTT) tests and colony-forming unit (CFU) by plating. The biofilm structure was designed using scanning electron microscopy and confocal laser scanning microscopy techniques. The extracellular matrix of P. brasiliensis and P. lutzii biofilms was extracted by sonication, and polysaccharides, proteins, and extracellular DNA (eDNA) were quantified. The RNA was extracted with the Trizol® reagent and quantified; then, the cDNA was synthesized to analyze the enolase expression, 14-3-3, FKS1, AGS1, GEL3, and KRE6 genes by real-time PCR. All strains of Paracoccidioides studied form a biofilm with more significant metabolic activity and biomass values in 144 h. The extracellular matrix of P. brasiliensis and P. lutzii had a higher content of polysaccharides in their composition, followed by proteins and eDNA in smaller quantities. The P. brasiliensis biofilm kinetics of formation showed greater expression of genes related to glucan’s synthesis and its delivery to the external environment in addition adhesins during the biofilm’s adhesion, initiation, and maturation. The GEL3 and enolase genes increased in expression within 24 h and during the biofilm maturation period, there was an increase in 14-3-3, AGS1, and FKS1. Furthermore, at 144 h, there was a decrease in KRE6 expression and an increase in GEL3. This study highlights the potential for biofilm formation for three species of Paracoccidioides and the main components of the extracellular matrix that can contribute to a better understanding of biofilm organization. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Multidrug-Resistant (MDR) Klebsiella variicola Strains Isolated in a Brazilian Hospital Belong to New Clones

Author

Campos, Tatiana Amabile de, primary, Almeida, Felipe Marques de, additional, Almeida, Ana Paula Cardoso de, additional, Nakamura-Silva, Rafael, additional, Oliveira-Silva, Mariana, additional, Sousa, Isabela Felix Alencar de, additional, Cerdeira, Louise, additional, Lincopan, Nilton, additional, Pappas, Georgios Joannis, additional, and Pitondo-Silva, André, additional

Published
2021
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4. Dynamics of Mono- and Dual-Species Biofilm Formation and Interactions Between Paracoccidioides brasiliensis and Candida albicans

Author

Oliveira, Lariane Teodoro, primary, Medina-Alarcón, Kaila Petronila, additional, Singulani, Junya de Lacorte, additional, Fregonezi, Nathália Ferreira, additional, Pires, Regina Helena, additional, Arthur, Rodrigo Alex, additional, Fusco-Almeida, Ana Marisa, additional, and Mendes Giannini, Maria José Soares, additional

Published
2020
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7. Incorporation of Nonyl 3,4-Dihydroxybenzoate Into Nanostructured Lipid Systems: Effective Alternative for Maintaining Anti-Dermatophytic and Antibiofilm Activities and Reducing Toxicity at High Concentrations

Author

Costa-Orlandi, Caroline Barcelos, primary, Serafim-Pinto, Aline, additional, da Silva, Patrícia Bento, additional, Bila, Níura Madalena, additional, Bonatti, Jean Lucas de Carvalho, additional, Scorzoni, Liliana, additional, Singulani, Junya de Lacorte, additional, Santos, Claudia Tavares dos, additional, Nazaré, Ana Carolina, additional, Chorilli, Marlus, additional, Regasini, Luis Octávio, additional, Fusco-Almeida, Ana Marisa, additional, and Mendes-Giannini, Maria José Soares, additional

Published
2020
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8. Effect of the Ethyl Acetate Fraction of Eugenia uniflora on Proteins Global Expression during Morphogenesis in Candida albicans

Author

Silva-Rocha, Walicyranison P., primary, de Azevedo, Matheus F., additional, Ferreira, Magda R. A., additional, da Silva, Julhiany de Fátima, additional, Svidzinski, Terezinha I. E., additional, Milan, Eveline P., additional, Soares, Luiz A. L., additional, Rocha, Keyla B. F., additional, Uchôa, Adriana F., additional, Mendes-Giannini, Maria J. S., additional, Fusco Almeida, Ana M., additional, and Chaves, Guilherme M., additional

Published
2017
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9. Alkyl Protocatechuate-Loaded Nanostructured Lipid Systems as a Treatment Strategy for Paracoccidioides brasiliensis and Paracoccidioides lutzii In Vitro

Author

Medina-Alarcón, Kaila P., primary, Singulani, Junya L., additional, Voltan, Aline R., additional, Sardi, Janaina C. O., additional, Petrônio, Maicon S., additional, Santos, Mariana B., additional, Polaquini, Carlos R., additional, Regasini, Luis O., additional, Bolzani, Vanderlan S., additional, da Silva, Dulce H. S., additional, Chorilli, Marlus, additional, Mendes-Giannini, Maria J. S., additional, and Fusco-Almeida, Ana M., additional

Published
2017
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11. Corrigendum: Impact of Resistance to Fluconazole on Virulence and Morphological Aspects of Cryptococcus neoformans and Cryptococcus gattii Isolates

Author

Rossi, Suélen A., primary, Trevijano-Contador, Nuria, additional, Scorzoni, Liliana, additional, Mesa-Arango, Ana C., additional, de Oliveira, Haroldo C., additional, Werther, Karin, additional, de Freitas Raso, Tânia, additional, Mendes-Giannini, Maria J. S., additional, Zaragoza, Oscar, additional, and Fusco-Almeida, Ana M., additional

Published
2016
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12. Virulence of Cryptococcus sp. Biofilms In Vitro and In Vivo using Galleria mellonella as an Alternative Model

Author

Benaducci, Tatiane, primary, Sardi, Janaina de C. O., additional, Lourencetti, Natalia M. S., additional, Scorzoni, Liliana, additional, Gullo, Fernanda P., additional, Rossi, Suélen A., additional, Derissi, Jaqueline B., additional, de Azevedo Prata, Márcia C., additional, Fusco-Almeida, Ana M., additional, and Mendes-Giannini, Maria J. S., additional

Published
2016
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13. Impact of Resistance to Fluconazole on Virulence and Morphological Aspects of Cryptococcus neoformans and Cryptococcus gattii Isolates

Author

Rossi, Suélen A., primary, Trevijano-Contador, Nuria, additional, Scorzoni, Liliana, additional, Mesa-Arango, Ana Cecilia, additional, de Oliveira, Haroldo C., additional, Werther, Karin, additional, de Freitas Raso, Tânia, additional, Mendes-Giannini, Maria J. S., additional, Zaragoza, Oscar, additional, and Fusco-Almeida, Ana M., additional

Published
2016
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14. An Intracellular Arrangement of Histoplasma capsulatum Yeast-Aggregates Generates Nuclear Damage to the Cultured Murine Alveolar Macrophages

Author

Pitangui, Nayla de Souza, primary, Sardi, Janaina de Cássia Orlandi, additional, Voltan, Aline R., additional, dos Santos, Claudia T., additional, da Silva, Julhiany de Fátima, additional, da Silva, Rosangela A. M., additional, Souza, Felipe O., additional, Soares, Christiane P., additional, Rodríguez-Arellanes, Gabriela, additional, Taylor, Maria Lucia, additional, Mendes-Giannini, Maria J. S., additional, and Fusco-Almeida, Ana M., additional

Published
2016
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15. Paracoccidioides-host Interaction: An Overview on Recent Advances in the Paracoccidioidomycosis

Author

de Oliveira, Haroldo C., primary, Assato, Patrícia A., additional, Marcos, Caroline M., additional, Scorzoni, Liliana, additional, de Paula E Silva, Ana C. A., additional, Da Silva, Julhiany De Fátima, additional, Singulani, Junya de Lacorte, additional, Alarcon, Kaila M., additional, Fusco-Almeida, Ana M., additional, and Mendes-Giannini, Maria J. S., additional

Published
2015
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16. Importance of adhesins in virulence of Paracoccidioides spp.

Author

de Oliveira, Haroldo C., primary, da Silva, Julhiany de Fátima, additional, Scorzoni, Liliana, additional, Marcos, Caroline M., additional, Rossi, Suelen A., additional, de Paula e Silva, Ana C. A., additional, Assato, Patrícia A., additional, da Silva, Rosângela A. M., additional, Fusco-Almeida, Ana M., additional, and Mendes-Giannini, Maria J. S., additional

Published
2015
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18. Multidrug-Resistant (MDR) Klebsiella variicola Strains Isolated in a Brazilian Hospital Belong to New Clones.

Author

de Campos TA, de Almeida FM, de Almeida APC, Nakamura-Silva R, Oliveira-Silva M, de Sousa IFA, Cerdeira L, Lincopan N, Pappas GJ Jr, and Pitondo-Silva A

Abstract

Klebsiella variicola is mainly associated with opportunistic infections and frequently identified as Klebsiella pneumoniae. This misidentification implies a wrong epidemiology result as well as incorrect attribution to K. pneumoniae as the etiology of some severe infections. Recently, huge efforts have been made to study K. variicola , however, the biological aspects of this species are still unclear. Here we characterized five K. variicola strains initially identified as K. pneumoniae , with a Vitek-2 System and 16S rRNA sequencing. One-step multiplex polymerase chain reaction and Whole Genome Sequencing (WGS) identified them as K. variicola. Additionally, WGS analysis showed that all the strains are closely related with K. variicola genomes, forming a clustered group, apart from K. pneumoniae and K. quasipneumoniae. Multilocus sequence typing analysis showed four different sequence types (STs) among the strains and for two of them (Kv97 and Kv104) the same ST was assigned. All strains were multidrug-resistant (MDR) and three showed virulence phenotypes including invasion capacity to epithelial cells, and survival in human blood and serum. These results showed the emergence of new K. variicola clones with pathogenic potential to colonize and cause infection in different tissues. These characteristics associated with MDR strains raise great concern for human health., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Campos, Almeida, Almeida, Nakamura-Silva, Oliveira-Silva, Sousa, Cerdeira, Lincopan, Pappas and Pitondo-Silva.)

Published
2021
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