1. Metabolic Stress Impairs Pericyte Response to Optogenetic Stimulation in Pancreatic Islets
- Author
-
Aurélien Michau, Chrystel Lafont, Paula Bargi-Souza, Yasmine Kemkem, Anne Guillou, Magalie A. Ravier, Gyslaine Bertrand, Annie Varrault, Tatiana Fiordelisio, David J. Hodson, Patrice Mollard, Marie Schaeffer, Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Universidade Federal de Minas Gerais = Federal University of Minas Gerais [Belo Horizonte, Brazil] (UFMG), Universidad Nacional Autónoma de México = National Autonomous University of Mexico (UNAM), Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), University of Oxford, Centre de Biologie Structurale [Montpellier] (CBS), ANR-13-JSV1-0001,BETA-DYN,Dynamiques vasculaires et cellulaires dans les îlots pancréatiques lors du diabète de type 1(2013), ANR-15-CE14-0012,Repro-Net,Impact du remodelage inter-réseaux sur la reproduction(2015), ANR-18-CE14-0017,GH-Gen,Origine et fonction du generateur hypophysaire de pulses d'hormone de croissance(2018), Guerineau, Nathalie C., Jeunes Chercheuses et Jeunes Chercheurs - Dynamiques vasculaires et cellulaires dans les îlots pancréatiques lors du diabète de type 1 - - BETA-DYN2013 - ANR-13-JSV1-0001 - JC - VALID, Impact du remodelage inter-réseaux sur la reproduction - - Repro-Net2015 - ANR-15-CE14-0012 - AAPG2015 - VALID, and APPEL À PROJETS GÉNÉRIQUE 2018 - Origine et fonction du generateur hypophysaire de pulses d'hormone de croissance - - GH-Gen2018 - ANR-18-CE14-0017 - AAPG2018 - VALID
- Subjects
[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism ,diabetes ,Endocrinology, Diabetes and Metabolism ,vessel ,imaging ,[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] ,[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism ,Islets of Langerhans ,Mice ,in vivo ,Diabetes Mellitus, Type 2 ,Stress, Physiological ,pericyte ,[SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] ,Animals ,pancreas ,Pericytes ,optogenetics - Abstract
Pancreatic islets are highly vascularized micro-organs ensuring whole body glucose homeostasis. Islet vascular cells play an integral part in sustaining adequate insulin release by beta cells. In particular, recent studies have demonstrated that islet pericytes regulate local blood flow velocity and are required for maintenance of beta cell maturity and function. In addition, increased metabolic demand accompanying obesity alters islet pericyte morphology. Here, we sought to explore the effects of metabolic stress on islet pericyte functional response to stimulation in a mouse model of type 2 diabetes, directly in the pancreas in vivo . We found that high fat diet induced islet pericyte hypertrophy without alterations in basal local blood flow. However, optogenetic stimulation of pericyte activity revealed impaired islet vascular responses, despite increased expression of genes encoding proteins directly or indirectly involved in cell contraction. These findings suggest that metabolic stress impinges upon islet pericyte function, which may contribute to beta cell failure during T2D.
- Published
- 2022