Despite becoming one of the main pressures on aquatic ecosystems globally, understanding of the ecological impacts of altered water-level regimes in lakes lags far behind that of other human disturbances (e.g. eutrophication, acidification)., We employed a multifaceted approach to explore the potential importance of water-level fluctuations (WLF) for the structure and functioning of littoral zones and multiple trait responses of primary producers and benthic consumers across a range of natural lakes., We found that lakes with high WLF had significantly more coarse littoral substrata with less coverage of macrophyte vegetation in the shallows than in lakes with low WLF. Lakes with high WLF also had greater proportions of motile diatom species and omnivorous benthic invertebrates in shallow waters, altered taxonomic and trophic structure of benthic consumers and more homogeneous algal and benthic invertebrate assemblages., Variation along the littoral depth gradient needs to be examined when assessing the impacts of hydrological pressures in lakes. We found that depth interacted with WLF in its effects on habitat structure and mediated the response of both producer and consumer benthic assemblages to WLF., Our results indicate that amplified WLF significantly affect both the structure and functioning of lake ecosystems. Given the growing importance of WLF as an increasingly pervasive pressure on lake ecosystems worldwide, our findings have important implications for the conservation and management of global aquatic biodiversity. Inclusion of both biological traits and sampling along a depth gradient in existing and in future monitoring programmes could improve significantly the ability to detect and predict effects of altered patterns of WLF on lake ecosystems. [ABSTRACT FROM AUTHOR]