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Your search keyword '"Suzuki, Masako"' showing total 39 results
Start Over Author "Suzuki, Masako" Journal forensic toxicology
39 results on '"Suzuki, Masako"'

9. Long-term stability of 24 synthetic cannabinoid metabolites spiked into whole blood and urine for up to 168 days, and the comparable study for the 6 metabolites in non-spiked real case specimens stored for several years

Author

Minakata, Kayoko, Hasegawa, Koutaro, Nozawa, Hideki, Yamagishi, Itaru, Miyoshi, Naotomo, Suzuki, Masako, Kitamoto, Takuya, Kondo, Minako, Watanabe, Kanako, and Suzuki, Osamu

Subjects
Human authentic specimens, LC–MS/MS, Blood and urine, Long-term stability, Deep-freezing, Synthetic cannabinoid metabolite
Abstract

Purpose The aim of this study is to investigate the stabilities of the 24 synthetic cannabinoid metabolites (SCMs) in blood and urine at various temperatures from –30 to 37℃ stored for 1 to 168 days. In addition, experiments of stabilities at lower temperatures and for much longer duration have been performed as described below.Methods The quantification was performed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The blank blood and urine spiked with SCMs and non-spiked real case (authentic) specimens were incubated at 37℃ up to 56 days and at 22, 4 or –30℃ up to 168 days. The non-spiked authentic blood and urine specimens were also stored at –30 or –80℃ for 1, 3 or 5 years to investigate stabilities during very long time frames.Results All the 24 SCMs were much more stable in urine than in blood at 37, 22 or 4℃. All 24 SCMs spiked into blood or urine were stable at –30℃ for up to 168 days. The 6 SCMs in the authentic specimens exhibited long stabilities at –30 or –80℃ for 3 to 5 years. Some tendencies were observed according to the relation between the structures of SCMs and their stabilities.Conclusions The long-term stabilities of 24 SCMs in spiked samples and those of 6 SCMs in the authentic specimens were examined using LC–MS/MS. SCMs were largely very stable and usable several years after storage at –30 or –80℃.

Published
2022

32. Sensitive quantification of BB-22 and its metabolite BB-22 3-carboxyindole, and characterization of new metabolites in authentic urine and/or serum specimens obtained from three individuals by LC–QTRAP-MS/MS and high-resolution LC–Orbitrap-MS/MS

Author

Minakata, Kayoko, primary, Hasegawa, Koutaro, additional, Nozawa, Hideki, additional, Yamagishi, Itaru, additional, Saitoh, Takeji, additional, Yoshino, Atsuto, additional, Suzuki, Masako, additional, Kitamoto, Takuya, additional, Suzuki, Osamu, additional, and Watanabe, Kanako, additional

Published
2018
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34. Sensitive quantification of 5F-PB-22 and its three metabolites 5F-PB-22 3-carboxyindole, B-22 N-5-hydroxypentyl and PB-22 N-pentanoic acid in authentic urine specimens obtained from four individuals by liquid chromatography-tandem mass spectrometry.

Author

Minakata, Kayoko, Hasegawa, Koutaro, Yamagishi, Itaru, Nozawa, Hideki, Kikura-Hanajiri, Ruri, Suzuki, Masako, Kitamoto, Takuya, Suzuki, Osamu, and Watanabe, Kanako

Abstract

Purpose: Urine is the most suitable specimen to collect from individuals because of noninvasiveness and relatively large volumes obtainable. In authentic urine specimens, however, synthetic cannabinoids having the structures of quinolinyl ester indoles, such as 5F-PB-22, PB-22 and BB-22, in unchanged forms as well as their metabolites, have not been quantified yet. Therefore, the aim of this study was to establish a sensitive analytical method for the quantification of 5F-PB-22 and its three metabolites 5F-PB-22 3-carboxyindole, PB-22 N-5-hydroxypentyl and PB-22 N-pentanoic acid in authentic urine samples in four cases. Methods: These compounds were extracted from β-glucuronide-hydrolyzed and unhydrolyzed urine via liquid-liquid extraction. The identification and quantification were performed using the QTRAP type of a liquid chromatography-tandem mass spectrometer. Results: The limits of detection were 3-30 pg/mL and their summed quantitation range was 10-10,000 pg/mL. The devised method was applied to quantify these compounds in authentic urine specimens obtained from four individuals. The levels of 5F-PB-22 were 5.1, 13.6, 94.7 and 470 pg/mL; those of 5F-PB-22 3-carboxyindole were 8.25, 3.39, 23.2 and 880 ng/mL; and those of PB-22 N-pentanoic acid were 12.0, 57.4, 959 and 2090 pg/mL, respectively, in four unhydrolyzed urine samples, and the levels of PB-22 N-5-hydroxypentyl could be quantified as 29.9 and 131 pg/mL in two unhydrolyzed urine samples. Conclusions: The 5F-PB-22 and its metabolites PB-22 N-5-hydroxypentyl have been detected from authentic human urine samples for the first time. Also, this is the first report dealing with the quantification of the three metabolites in human urine samples. [ABSTRACT FROM AUTHOR]

Published
2018
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