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Migrant deaths have persistently intensified in the last 30 years, followed by inconsistent engagement of official policies to tackle the lack of identifications. Forensic anthropologists have been increasingly involved in the recovery, analysis and identification of human remains associated with migrants, and are therefore the most well-versed practitioners in raising awareness of policy makers about this plight. This review describes the current state-of-the-art of the forensic anthropological practice and research on unidentified migrants. The focus of the review is twofold: one section explores the current legal, logistical, and administrative issues related to management and identification activities, primarily in Europe and North America. The second section addresses the scientific contributions and new challenges that forensic anthropologists face when analyzing skeletal remains from underrepresented and unreferenced population groups. According to this literature review, forensic anthropologists are the professionals most actively involved in addressing the issue of unidentified migrants. Despite significant efforts, several challenges still hinder identifications in this context, including the inability to create comprehensive databases for collecting postmortem and antemortem data. Recently, scientific research in forensic anthropology has begun to address the limitations in developing reliable biological profiles for deceased migrants, as these groups are often inadequately represented in existing skeletal reference populations. Finally, this review highlights the global efforts of the scientific community to integrate these victims into a well-established identification system, aiming to foster public responsibility and promote the adoption of unified and innovative strategies to comprehensively address and resolve this social, scientific, and humanitarian crisis. • Migrant deaths are a key issue in forensic anthropology. • Identification is challenged by lack of comprehensive data collection. • New tools and methods are needed for reliable migrant identification. • Anthropologists should raise awareness and influence identification policies. [ABSTRACT FROM AUTHOR]

This study introduces a comprehensive inventory of 54 fingerprint minutiae and their variations aimed at standardizing characteristic point identification within forensic science. By analyzing a strategically sampled collection of fingerprints from the Brazilian Federal Police database, stratified by sex and geographic location, our research uncovers the complex interplay between various levels of fingerprint details (L1D, L2D, and L3D) and demographic factors such as sex and finger type. The sample encompassed the entire proposed list of minutiae, affirming the diversity and representativeness of the Brazilian populace, which had, for the first time, its minutiae frequency distributions studied. This investigation proposes a systematic approach for enhancing fingerprint identification accuracy by minimizing data categorization losses and lays the groundwork for more uniform comparative studies in the field. Our findings, derived from a review of contemporary studies and traditional identification manuals, suggest a step towards establishing a universally accepted standard for fingerprint minutiae classification. • The first study of fingerprint minutiae distribution in the Brazilian population. • Considers simultaneously the largest list of minutiae types already studied. • Distributions of minutiae by sex, general patterns, and finger type. • Significant statistical relationships between L2D, L1D/L3D and finger types. [ABSTRACT FROM AUTHOR]

The aim of this study is to validate the FIDBv2 online procedure for adult age-at-death estimation using root dentine translucency (RDT) and periodontal retraction (PR) of single-rooted teeth in a worldwide sample. The sample includes 4810 teeth of 2559 individuals from 16 countries of America, Europe and Asia. Bias and inaccuracy between documented (DA) and estimated ages (EA) were calculated. Pearson and Intraclass Correlation Coefficients were computed to assess the strength of agreement between pairs of data, while Kolmogorov-Smirnov Z was used to evaluate the statistical significance of the differences. The percentages of correctly estimated cases within different age ranges were obtained to find trends in the reliability of the results. Most of the biases (-4.61–1.31 years) and inaccuracies (4.81–9.72 years) are low. The dispersion of EA increases with age and almost all the DA-EA correlations are above 0.75. DA-RDT and DA-PR correlations are positive, most of the former being high (0.74–0.91), and the latter being low (0.11–0.54). The highest percentages of correct estimations are identified for the ±7.5 and ±10 years ranges, and most comparisons of bias and inaccuracy between countries are non-significant. The high correlations between DA and EA suggest that the method is robust and reliable for a global application. Mean errors are low, with the best results found in the 30–69-year-old cohort. This research supports that the method is effective and accurate for age estimation in forensic contexts worldwide, thus reaffirming it is a generalizable procedure locally and internationally. • The FIDBv2 method is effective for age estimation in forensic contexts worldwide. • A strong association of RDT with age-at-death was identified in all the samples. • The method is robust and reliable, with minimal impact of interpopulational variation. • Its relevance in the analysis of commingled and damaged samples is underscored. • The results support incorporating the FIDBv2 method within Daubert standards. [ABSTRACT FROM AUTHOR]

Evaluating sharp force trauma (SFT) injuries to bone inflicted by a larger class of chopping/hacking tools (i.e., swords, axes, hatchets, machetes, and cleavers) is a growing area within the field of forensics. Previous studies have demonstrated promise regarding tool differentiation through the utilization of microscopic cut mark characteristics. However, this is an area that warrants additional investigation as there has yet to be a comprehensive study that incorporates the majority of these microscopic chopping/hacking cut mark characteristics into one analysis. The purpose of the current research was to analyze a large sample of microscopic cut mark characteristics derived from the current chopping/hacking literature to determine if differentiation of tool type could be made based on the microscopic cut mark characteristics. An additional goal of the current research is to develop standardization guidelines for the assessment of cut marks, focusing on a comprehensive suite of microscopic cut mark characteristics commonly observed in the existing literature. The skeletal sample for the current research was derived from a previous macroscopic cut mark characteristic experiment where trauma was inflicted to 20 partially fleshed domestic pig (Sus scrofa domesticus) limbs utilizing four chopping/hacking tools (i.e., axe, hatchet, machete, and cleaver) as well as a large carving knife for comparison. Cut marks were evaluated for 15 microscopic cut mark characteristics to assess statistical significance. Utilizing a chi-square analysis, nine of the 15 microscopic cut mark characteristics demonstrated statistically significant differences in relation to the tool utilized, indicating moderate to relatively strong effect sizes. For example, it should be possible to use a combination of microscopic cut mark characteristics to potentially indicate which chopping/hacking tool inflicted trauma when analyzing bones. In particular, examining the characteristics such as regularity of the cut mark edges along with the occurrence of uprising, fossae/depressions, and microscopic fractures can suggest or exclude tool class within forensic contexts. • Chopping/hacking trauma is a subcategory of sharp force trauma. • Microscopic cut mark characteristics evaluated from chopping/hacking tools. • Nine of 15 microscopic cut mark characteristics indicated statistical significance. • Patterns of microscopic cut mark characteristics may suggest or exclude tool class. [ABSTRACT FROM AUTHOR]

Over the last forty years an indeterminate number of persons, ranging from thousands to tens of thousands, have died along the US-Mexico border during migration, fleeing poverty, armed conflict, situations of violence, and disasters. An accurate accounting of migrant deaths along the southern US border is the first step toward an understanding of the extent and the contributing factors of these deaths. In this article, we describe a key aspect of our collaborative work aimed at developing a more representative account of migrant mortality along the southwestern US border: the determination of criteria for inclusion of specific forensic cases as "migrant." Our intention is not to propose a definition of "what is a migrant death" applicable to all contexts and situations but rather one specific to the US-Mexico border region. Our main impetus is to build and launch a web portal to track and map migrant deaths at the US-Mexico border. The criteria we have identified are based on an examination of death data collected by various agencies in the four border states (California, Arizona, New Mexico, and Texas) and at the federal level by the National Missing and Unidentified Persons System (NamUs). They include a) context of human remains discovery; b) identification media/documentation; c) geographic setting; and d) personal effects. Taken together, these criteria will facilitate our determination, case by case, of the probability that human remains found along the United States side of the border may be from a person in the context of migration. • We define a set of four criteria for distinguishing migrant deaths. • Criteria highlight jurisdictional observations of migrant deaths. • The definition of migrant death proposed applies to the US-Mexico border region. [ABSTRACT FROM AUTHOR]

More than 1200 civilians and military were killed in cities and villages by the Hamas attack on October 7th, 2023. The bodies and body-parts had to be identified and released for burial. This report outlines the challenges and mode of operation of the massive disaster victim identification (DVI) efforts. Bodies were delivered to a central mortuary. Each body was coded and undressed for external inspection and documentation of physical elements. Digital fingerprints were recorded and blood or toenails sampled for DNA. Odontology exams were performed by dentists describing dentition, aided by computerized tomography (CT). Whole-body CT was performed in cases the bodies were disfigured or burned from the second week. Simultaneously, families of missing civilians provided physical elements to the police to extract the DNA for antemortem documentation. The police took the responsibility over the reconciliation, which was based on comparison of the ante-mortem and post-mortem fingerprints, aided by DNA profile matching, odontology examination, clinical and/or radiological findings performed by forensic practitioners. Secondary identification elements were used assure the families regarding the identification. Precise scientific identification a was a priority, even if it slowed the rate of bodies release. Families were allowed to view their relative either at the mortuary or before burial. The DVI process required cooperation between several governmental agencies and police. To maximize the effectiveness, a synchronized approach should be adopted, specifying communication channels between the partners and dividing the responsibilities. The DVI should be led by a single, experienced authority to ensure interdisciplinary teamwork. This catastrophe required personal resilience of the teams for rapid and efficient functioning and communication between the partners., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

This study investigated if microscopic surface features captured with a scanning electron microscope (SEM) effectively discriminate fracture timing. We hypothesized that microscopic fracture characteristics, including delamination, osteon pullout, and microcracks, may vary as bone elasticity decreases, elucidating perimortem and postmortem events more reliably than macroscopic analyses. Thirty-seven unembalmed, defleshed human femoral shafts from males (n=18) and females (n=2) aged 33-81 years were fractured at experimentally simulated postmortem intervals (PMIs) ranging from 1 to 60 warm weather days (250-40,600 ADH). A gravity convection oven was used to approximate tissue decomposition at 37 C and 27 C, and the resulting heat-time unit (accumulated degree hours, or ADH) was used to examine fractures in elastic/wet versus brittle/dry bone. The bones were fractured with a drop test frame using a three-point bending setup, sensors were used to calculate fracture energy, and high-speed photography documented fracture events. The following data were collected to relate fracture appearance to the biomechanical properties of bone: PMI (postmortem interval) length in ADH, temperature, humidity, collagen percentage, water loss, bone mineral density, cortical bone thickness, fracture energy, age, sex, cause of death, and microscopic fracture feature scores. SEM micrographs were collected from the primary tension zones of each fracture surface, and three microscopic fracture characteristics were scored from a region of interest in the center of the tension zone: percentage of delaminated osteons, percent osteon pullout, and number of microcracks. Multiple linear regression showed that microscopic fracture surface features are strong predictors of ADH (adjusted R-squared=0.67 for the 0 - 40,000 ADH samples; adjusted R-squared=0.92 for the 0-16,000 ADH samples). Osteon pullout is the single best predictor of ADH. Additionally, water loss is the primary driver of bone elasticity changes in low ADH samples, while collagen fibers appear to remain intact until later in the postmortem interval (approximately 40,000 ADH in this study). The results of this study indicate microscopic fracture surface analysis detects the biomechanical effects of decreased elasticity more reliably and with greater sensitivity than macroscopic analysis., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Stature estimation is a core component to the biological profile in forensic anthropology casework. Here we provide mathematical equations for estimating stature for contemporary American Indians (AI), which currently are lacking in forensic anthropology. Drawing on postmortem computed tomography data from the New Mexico Decedent Image Database we regressed cadaveric length on four long bone length measures of the tibia, femur, and humerus to produce 11 combinations of models. Separate regression models were calculated for the entire pooled sample, by sex, broad AI language groups, and age + sex subsamples and compared. Sex-specific models were statistically better than general models, which were more accurate than language group and age + sex models. Equations were created for general and sex-specific models. Application to an independent test sample demonstrates the equations are accurate for stature estimation with overestimates of less than 1 cm. The equations provide similar levels of precision to stature estimation programs like the FORDISC 3.0 module and other stature equations in the literature. We provide recommendations for equation use in casework based on our results. These equations are the first for estimating stature in contemporary AI. This paper demonstrates the appropriateness of these newly created stature equations for use in New Mexico and the surrounding region. • This research provides the first stature estimation equations based on contemporary American Indians. • Stature equations derive from postmortem CT scans of maximum humeral, tibial, and femoral length and femoral bicondylar length. • Sex-specific models perform better than general models. • An independent test sample indicates the newly created equations are accurate and precise. • We present updated stature estimation equations for Southwest American Indians for use in forensic anthropology casework. [ABSTRACT FROM AUTHOR]

Dismemberment and subsequent burning are common methods employed in an attempt to conceal or destroy evidence. While kerf characteristics can be utilised to identify tool(s) used for dismemberment, further research is necessary to assess the effect of burning on these characteristics. In this study, a back (tenon) saw (13 teeth per inch) was used to manually inflict trauma on Ovis aries de-fleshed femur bones (n = 18). Three different cut marks (shallow false start, incomplete cut and complete transection) were made on the mid-shaft of each bone. Subsequently, the bones were burned for 20 minutes in a muffle furnace. Three burn temperatures were assessed: 400 °C, 600 °C and 800 °C. Saw mark characteristics of each cut type were assessed and compared pre- and post-burning. All pre-existing trauma was recognisable post-burning; however, metric and morphological alterations were apparent. An increase in kerf width was observed at 600 °C in false start lesions and 800 °C in incomplete cuts. Breakaway spur thickness decreased post-burning (at 400 °C and 800 °C) but length was not significantly affected. Mean inter-striation distance decreased post burning at all temperature groups. Saw marks were distinguishable from heat-related fractures across all temperature groups. One false start lesion was obliterated at 800 °C. Exit chipping, pull-out striae as well as striation regularity appeared to be more enhanced after heat exposure. These alterations indicate a temperature-dependent impact on these characteristics. Further research is necessary to assess the role of burn duration. • Increased fracturing and fragility in burnt bones with increased burn temperature. • Saw mark characteristics more evident in burned compared to unburnt bones. • Significant increase in kerf width and decrease in kerf depth at 800°C. [ABSTRACT FROM AUTHOR]

Three-dimensional (3D) structured light scanning is a beneficial documentation technique in forensic anthropology because such models facilitate continued analysis and data sharing; they can also be 3D printed for demonstrative purposes in legal proceedings and training, without risk of damage to the original skeletal material. As its application in forensic anthropology is relatively novel, the aim of the present study is to statistically evaluate the dimensional accuracy of 3D structured light scans and 3D prints for ten bone types, including the cranium, mandible, 2nd cervical vertebra (C2), clavicle, scapula, capitate, 2nd metacarpal, os coxae, femoral head, and patella. Standard linear measurements are acquired in each physical bone, 3D virtual model, and 3D print of the same bone specimen. Variances between measurements of physical, virtual, and printed bones are quantified using the technical error of measurement (TEM), relative TEM (rTEM), and coefficient of reliability (R). Measurements acquired in the virtual models and prints were found to be within ±2 mm average of the same measurements in the physical bones, with a tendency to underestimate true value. rTEM and R values for the virtual clavicle, capitate, scapula and C2, and rTEM for the printed clavicle and capitate, were comparatively less reliable than for other bone types; although all bones were reproduced to within acceptable anthropological error standards (rTEM≤5 %; R≥0.95). This study reaffirms the use of 3D structured light scanning and 3D printing to complement traditional skeletal documentation in forensic anthropology. • Forensic methods require measures of accuracy and precision. • Compared linear measurements from physical bones, 3D surface scans and 3D prints. • Virtual models and prints within ±2 mm average accuracy of physical human bones. • Minimal differences in accuracy between ten different bone types. • Supports application of 3D scanning and printing in forensic anthropology. [ABSTRACT FROM AUTHOR]

Spain suffered a Civil War between 1936 and 1939 that ended with the victory of the National Forces led by General Franco. Once the Spanish Civil War ended, 2238 subjects were executed and buried in several mass graves in the Cemetery of Paterna, one of Spain's largest mass grave sites. Efforts to locate and identify all the victims of the mass graves of the Paterna cemetery are ongoing, but the actual data of the percentage of DNA identifications remains uncertain. Following this, we conducted a meta-research study including 15 mass graves and 933 subjects to determine the DNA identification rates in the mass graves of the Paterna cemetery. We found that the total proportion of identified subjects in the mass graves was 15.9 % (95 % CI: 10.0-22.9). Moreover, we found that the model between the identification success rate (ISR) and the number of relatives that donated DNA (NRTDD) in the mass graves of the cemetery of Paterna was ISR = NRTDD -0.424 . Results obtained about the proportion of identified subjects and the model between the ISR and the NRTDD imply the need for a scientific reflection between all the research groups involved in the identification tasks to modify deficiencies and update identification protocols to obtain better future results., Competing Interests: Declaration of Competing Interest Javier Iglesias-Bexiga, Marisol E. Schwab, Alejando Calpe, Gema López-García, Eloy Ariza, and Miguel Mezquida have participated as members of the ArqueoAntro Scientific Association in the identification tasks of some mass graves of the Paterna Cemetery included in this study, and have also provided the current identification rates reports of that mass graves with complete anonymization of the personal data of the victims., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

The pars basilaris forms a central component of the immature basicranium and owing to its resilience to post-mortem and taphonomic changes, holds significance across evolutionary, clinical, and forensic contexts. While size and shape parameters of the pars basilaris have been investigated, little is known about the influence of the underlying bone mineral density on the morphometry of this bone during growth. This study aimed to investigate the development and growth of the pars basilaris with specific reference to changes in bone density patterning and development of osteological features, during the prenatal and early postnatal periods of life. A total of 109 pars basilari were sourced from the Johannesburg Forensic Paediatric Collection, University of the Witwatersrand, South Africa. The study sample was subdivided into early prenatal (<30 gestational weeks), prenatal (30-40 gestational weeks) and postnatal (birth to 7.5 months) groups and micro-CT scanned to assess bone mineral density patterns across seven regions of interest. Size and shape changes were analysed using 11 digitized landmarks and geometric morphometrics. When comparing across age groups, the assessed dimensions increased with growth manifesting as a deepening at the anterior border of the foramen magnum, development of the lateral angles and widening of the bone at the lateral projections and spheno-occipital synchondrosis. However, no significant changes in the distribution of bone mineral density were observed. An appreciation of morphological changes and bone quality at specific growth sites in the pars basilaris is essential when analyzing remains of unknown provenance for the purposes of identification in disaster victim settings., Competing Interests: Declaration of Competing Interest None, (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Teeth are considered unique as fingerprints for identification purposes. Their structure and resilience mean they can remain for thousands and millions of years withstanding extreme conditions, including burning. During burning, bones undergo carbonization at approximately 400°C and calcination at approximately 700°C. This study aimed to investigate the effects of carbonization and calcination on dental tissue. It involved nondestructive analyses of 58 extracted human teeth before and after burning, using x-ray diffraction, micro-CT, and high-resolution confocal microscopy. The results revealed that during carbonization, dentin volume decreased in two thirds of the sample, accompanied by crack formation and significant reduction in hydroxyapatite crystal size (p<0.001). During calcination, dentin volume decreased in all teeth, along with a significant deepening of the cracks (p<0.001), while enamel crystal size increased slightly. Initial changes in teeth occurred at lower temperatures than had once been assumed, as indicated by the cracks during carbonization, and there was up to a 36% decrease in dentin volume during calcination, which should be considered when measuring burnt teeth. The results of this research provide new insight into understanding dental tissue response to burning. Thus, dental remains may contribute to the knowledge needed to reconstruct anthropological and forensic scenarios involving burning. • During carbonization dentin volume and hydroxyapatite crystals decrease in size. • During calcination dentin volume continues to decrease. • Enamel crystals show a slight size increase during calcination. • Cracks deepen with increased temperature from microscopic to visible. [ABSTRACT FROM AUTHOR]

Intimate partner violence (IPV) is a global human rights issue that affects approximately 25% of women and 10% of men and is the leading cause of homicides of women worldwide. Multiple interventional studies have been conducted to screen for IPV; however, fractures associated with intimate partner homicide (IPH) have not been studied from a forensic anthropological perspective. Therefore, this study uses computed tomography scans of IPH victims (n=33) obtained from the New Mexico Office of the Medical Investigator to 1) classify and quantify perimortem craniofacial blunt force fractures, and 2) compare the IPH-related fractures to those associated with non-lethal IPV using previously published studies. The results indicate that IPH cases presented similarly to non-lethal IPV cases in that they were concentrated on the middle and lower face, but fractures were more frequent in the upper face and cranial vault in IPH cases. While IPH cases showed more fractures, they were not necessarily associated with extensive fracturing, as 75.8% of IPH victims had five or fewer fractures—the most common being comminuted and linear fractures, comprising 93.8% of IPH-related fractures. As IPV is significantly underreported, understanding the nuances of fracture patterns associated with IPH can help to aid holistic forensic investigations. • Homicide and survived cases presented similarly in the middle and lower face. • Homicide cases exhibited more cranial vault fractures. • Extensive fracturing is not associated with homicide cases. • The use of weapons increased in homicide cases. • Linear and comminuted fractures were observed most frequently. [ABSTRACT FROM AUTHOR]

Forensic anthropologists are often confronted with human remains that have been damaged due to trauma, fire, or postmortem taphonomic alteration, frequently resulting in the fracture and fragmentation of skeletal elements. The augmented reality (AR) technology introduced in this paper builds on familiar 3D visualization methods and utilizes them to make three dimensional holographic meshes of skeletal fragments that can be manipulated, tagged, and examined by the user. Here, CT scans, neural radiance fields (NeRF) artificial intelligence software, and Unreal Engine production software are utilized to construct a three-dimensional holographic image that can be manipulated with HoloLens™ technology to analyze the fracture margin and reconstruct craniofacial elements without causing damage to fragile remains via excessive handling. This allows forensic anthropologists a means of assessing aspects of the biological profile and traumatic injuries without risking further damage to the skeleton. It can also be utilized by students and professional anthropologists to practice refitting before reconstructing craniofacial fragments if refitting is necessary. Additionally, the holographic images can be used to explain complicated concepts in a courtroom without the emotional response related to using bony elements as courtroom exhibits. • Craniofacial reconstruction aids with the biological profile and signs of trauma. • CT-based AR craniofacial reconstruction can reduce bone damage from overhandling. • Neural resonance fields (NeRF) can rapidly convert CT scans into AR visual content. • AR visualization minimizes damage from manual manipulation. • AR allows the viewer to assess the skeleton in three rather than two dimensions. [ABSTRACT FROM AUTHOR]

Population overlap and the variation within and among populations have been globally observed but is often difficult to quantify. To achieve this, numerous different methods need to be explored and validated to assist with the creation of an accurate biological profile. The current lack of databases for postcranial macromorphoscopic traits indicates the need to further investigate if the method can be employed repeatably in a forensic context. The current study aimed to assess the prevalence of eleven postcranial macromorphoscopic traits in a South African sample. A total of 271 postcrania of adult black, coloured, and white South Africans were assessed. The intra- and inter-observer agreement ranged from fair to almost perfect except for the accessory transverse foramen of C1, which had poor agreement between observers. Only seven traits differed significantly between at least two of the groups. Univariate and multivariate random forest models were created to test the positive predictive performance of the traits to classify population affinity. The classification accuracies for the univariate models ranged from 33.3% to 53.0% and ranged from 54.6% to 62.1% for the multivariate models. Based on the variable importance, the traits assessing spinous process bifurcation were the most discriminatory variables. The results indicate that the postcranial MMS approach does not outperform current methods employed to estimate population affinity. Further research needs to be done for the method to have practical applicability for medicolegal casework in South Africa. • This study evaluated postcranial macromorphoscopic traits in three South African groups. • Experience with the traits improves the observer repeatability of the method. • Numerous trait variations were observed that may complicate scoring. • Multivariate models presented fair accuracies ranging between 54.6% and 62.1%. • The postcranial traits were not an accurate indicator of population affinity in South Africa. [ABSTRACT FROM AUTHOR]

Radiographs regularly aid in identifications by comparing antemortem and postmortem images. The forensic community has widely accepted this method because radiographic images are low in cost, easily transferable, and generally believed to be uncomplicated to assess. However, there is a dearth of diverse, modern radiographic datasets as well as a deficiency in assessing population frequencies of nonmetric traits via radiographs. Further, radiographic comparisons, although typically required as a core competency of practicing forensic anthropologists, do not have standard training and education requirements. To understand the diversity and representation within an opportunistic dataset, we provide a case study of the demographics of decedents identified via radiographic comparison at the Clark County Office of the Coroner/Medical Examiner (CCOCME) in Las Vegas, NV, USA from 2017 to 2020. Additionally, we examined each region of the body used to make radiographic identifications. The sample was majority Caucasian and male, with a mean and median age at death of 64.9 years and 67.0 years, respectively. Our results indicated that this sample was not representative of the greater Clark County population. The most common body region used for comparisons was the chest, followed by the head and neck, pelvis, dentition, and the extremities. Thus, large generalizations made from these types of datasets must be implemented with caution due to the relative lack of diversity and representation. As well, current discussions regarding training and education of forensic anthropologists as they relate to radiographic comparisons are explored. • Radiographic identification may be limited for those from marginalized groups. • Identifications made via radiographs are primarily from the chest and head regions. • Caution should be applied when using opportunistic casework to generate generalizable methods. • Radiographic comparisons, particularly of the chest, should be included in forensic anthropologists' training and education. [ABSTRACT FROM AUTHOR]

The morphological assessment of the pubic symphysis using the Suchey-Brooks method is considered a reliable age at death indicator. Age at death estimation methods can be adapted to the images obtained from post-mortem computed tomography (PMCT). The aim of this study is to evaluate the utility of pubic symphysis photorealistic images obtained through Global illumination rendering (GIR) for age at death estimation from whole-body PMCT and from focused PMCT on the pubic bone. We performed virtual age at death estimation using the Suchey Brooks method from both the whole-body field of view (Large Field of View: LFOV) and the pubis-focused field of view (Small and Field of View: SFOV) of 100 PMCT. The 3D photorealistic images were evaluated by three forensic anthropologists and the results were statistically evaluated for accuracy of the two applied PMCT methods and the intra- and inter-observer errors. When comparing the two acquisitions of PMCT, the accuracy rate reaches 98.5% when using a pubic-focused window (SFOV) compared to 86% with a whole-body window (LFOV). Additionally, the intra- and inter-observer variability has demonstrated that the focused window provides better repeatability and reproducibility. Adding a pubic-focused field of view to standard PMCT and processing it with GIR appears to be an applicable technique that increases the accuracy rate for age at death estimation from the pubic symphysis. • Age estimation using the Suchey Brooks method from post-mortem computed tomography. • Comparison of Large Field Of View (LFOV) and Small Field Of View (SMFOV). • Accuracy ranges from 86% with LFOV to 98.5% with SMFOV. • Virtual images processed with Global Illumination Rendering (GIR) give excellent results for age estimation. [ABSTRACT FROM AUTHOR]

Vertebrate scavengers frequently affect forensic casework by feeding on human remains or by scattering body parts and bones. Therefore, animal activity can influence complete recovery of bodies, trauma analysis, and the estimation of the postmortem interval (PMI), potentially hampering identification of the deceased and elucidation of the perimortem circumstances. Experimental research is well suited to investigate scavengers and their impact on carcasses over time, generating knowledge on the forensic relevance of certain scavenger species or communities. However, there are currently no systematised standards to conduct these investigations with a forensic focus, impeding comparison and synthesis of the studies. In our work, we performed a systematic literature review and found 79 publications featuring terrestrial experiments on vertebrate scavenging and/or scattering within a forensic context. We extracted 21 variables describing the study environment, experimental design and the specimens. The results show that there is considerable inconsistency in the study designs and that some of the variables are insufficiently reported. We point out research questions and areas that require attention in future studies, stressing the importance of infrequently mentioned or applied variables. Furthermore, we recommend guidelines to include and report a list of variables in forensic scavenging and scattering experiments. These guidelines will help standardising future research in the field, facilitating inter-study consolidation of results and conclusions, and consequently, inform forensic casework. • Vertebrate scavengers are an important taphonomic variable. • We conducted a systematic literature following PRISMA guidelines. • There are 79 publications on forensic scavenging and scattering experiments. • Study designs are inconsistent and methodology is often poorly reported. • We provide a guideline to standardize future scavenging and scattering experiments. [ABSTRACT FROM AUTHOR]

Characterization of human saliva through Next-Generation Sequencing has emerged as a valuable tool for understanding the complex microbial communities residing in the oral cavity. This study aims to investigate the habit-based variations in the salivary microbiome using Next-Generation Sequencing technology. Saliva samples were collected from a diverse population representing different habits, including smoking, alcohol consumption, and vegan diet. The DNA from the samples was extracted, and the V3-V4 region of the 16 S rRNA gene was amplified for Next-Generation Sequencing analysis. The obtained sequences were processed and analysed using bioinformatics tools to determine the microbial composition and diversity. Preliminary results revealed distinct microbial profiles associated with different habits, indicating the potential influence of different habits on the salivary microbiome. Smokers exhibited a higher abundance of certain pathogenic bacteria, while alcohol consumers showed alterations in microbial diversity compared to non-consumers. Furthermore, individuals with vegan diet demonstrated an increased prevalence of specific bacteria. These findings highlight the significance of habit-based characterization of the salivary microbiome and its potential implications in the presence of certain bacteria. Understanding the relationship between habits and the salivary microbiome could contribute to developing personalized approaches for estimating and identifying any particular individual. Further research is warranted to explore additional factors and expand the scope of habit-based analysis in saliva-based microbial characterization through Next-Generation Sequencing. • Research on salivary bacteria identification is vital for forensic investigations. • Distinguishing habit-age and gender-specific bacteria aids in profiling individuals. • Enhanced accuracy in age and gender estimation can aid in criminal identification. • Contributes to the advancement of forensic science and strengthens legal processes. • Promises to improve the reliability of forensic evidence and help solve complex cases. [ABSTRACT FROM AUTHOR]

This paper presents some of the more commonly encountered non-metric traits and minor anatomical variants in the adult human skeleton that can mimic or be mistaken for trauma. Distinguishing non-metric traits is contingent upon both a knowledge of potential non-metric traits as well as the normal developmental timing, location, and anatomy of maturational markers in the human skeleton. Distinguishing non-metric traits from trauma in dry bone is an essential component in establishing an accurate and thorough forensic analysis of human remains, especially as it deals with antemortem and perimortem trauma, and postmortem damage. [ABSTRACT FROM AUTHOR]

Forensic pathology and forensic anthropology are inherently related fields. The observations made during the autopsy and the conclusions from the anthropological analysis highlight the complementarities between the two approaches. In this review, gunshot wounds, sharp force injuries, chop wounds, blunt trauma, burned bodies, mechanical asphyxia and dismemberment will be analyzed from both perspectives. The result of this systematic review is that the conclusions stemming from the presence or absence of bone injuries must be very cautious because the observation of severe bone lesions does not always mean that this trauma was the cause of death; conversely, traumatic death, and even homicide, can be associated with a complete absence of bone lesions. [ABSTRACT FROM AUTHOR]

Several studies have indicated that the time of onset of specific age-related changes throughout the human skeleton differs between populations. In this study the well-known Suchey-Brooks pubic symphyseal age estimation method was investigated to assess its performance in a white South African population. A total of 184 well-preserved os coxae were subjected to blind phase analysis, using the Suchey-Brooks descriptions and pubic symphyseal casts. The method performed well in a white South African population with statistically significant moderate positive correlations and relatively low biases in both males and females. However, due to the underperformance of the method in older individuals, new age ranges for phases IV through VI were created that are specific to the South African white population. This study indicated that Suchey-Brooks method can be used in a white South African population using the age ranges for phases I through III provided by Brooks and Suchey (1990), and the population specific age ranges for phase IV and V provided in this study. [ABSTRACT FROM AUTHOR]

An evaluation of the development of a child's skeleton and estimation of bone age provides an insight into a child's overall maturation. This study aimed to introduce a contemporary method for assessing bone age of Australian children using formulae incorporating carpal areal measurements. The standards introduced in this study can be used to assess the developmental status of Australian children who may be affected by growth-related illnesses. Additionally, in situations where the living age of a subadult is unknown, methodologies to accurately estimate age are required, particularly in the Western world where knowledge of the age of an individual is necessary for legal reasons. The sample consisted of retrospective hand and wrist radiographs acquired from 541 children (females: 246, males: 295) aged from birth to 20 years. Using the DICOM viewer Weasis, the carpal area ratio (B.Ar/T.Ar) was calculated for each individual radiograph by measuring the carpal bone area (B.Ar) and total tissue area of the carpus (T.Ar). A changepoint regression model demonstrated that the model constructed in this study was the most accurate in the younger age groups and was able to accurately determine whether a child was under 12 years if female and 13 years if male. A rapid acceleration of growth was observed at approximately 12-13 years in our sample, which may represent the onset of the pubertal growth spurt; this resulted in a high data variance and low model prediction accuracy in female and male children older than 12 and 13 years, respectively. [ABSTRACT FROM AUTHOR]

This pilot study examines the applicability of osteometric models for addressing commingled remains, which were originally developed for dry specimens, on 3-Dimensional bony elements in relation to a modern cadaveric population. A total of 70 bony elements (humeri, radii, ulnae, femora, tibiae and fibulae) were segmented and virtually reconstructed from cadaveric whole-body CT scans. Linear measurements were taken (using MeshLab v.2016.12) of the 3-Dimensional elements and osteometric models for sorting applied to them (Byrd and Adams, 2003). This study showed that on the selected specimens the quality of the surface of the reconstructed specimens compromised the efficacy of the models, and consequently the reliability of the results. [ABSTRACT FROM AUTHOR]

Child pornography on the internet is one of the crimes with highest increasing incidence worldwide. In this specific type of crime, the victim's age has important legal implications. To overcome the lack of scientifically established protocols, European researchers explored and proposed a set of facial proportions obtained from images to estimate age. Within a validation scope, the present study aimed to test the applicability of morphological proportions from frontal photographs of the face to estimate age in a Brazilian population. Standardized frontal view photographs from 800 Brazilians (400 females and 400 males) homogeneously distributed in 4 age groups (6, 10, 14 and 18 years ± 30 days) were randomly selected from a Civilian Database of the Brazilian Federal Police. The selected images were analyzed with the aid of cephalometric landmarks. After landmarking the photographs, eighteen metric relations pre-established by Cattaneo et al. (C. Cattaneo, Z. Obertová, M. Ratnayake, L. Marasciuolo, J. Tutkuviene, P. Poppa, D. Gibelli, P. Gabriel, S. Ritz-Timme, Can facial proportions taken from images be of use for ageing in cases of suspected child pornography? A pilot study, Int. J. Leg. Med. 126 2012 139-144) were assessed and analyzed through parametric statistical tests and discriminant analysis. Two indexes had a moderate correlation with age, while ten had a weak correlation and six were not correlated with age. Eleven indexes were statistically different between sexes (p < 0.05). The discriminant analysis showed that 49.6% of the pooled sample was correctly classified into the respective age group. This percentage increased when each sex was analyzed separately: 50.2% for females and 53.5% for males. The set of facial proportions from frontal images proposed by Cattaneo et al. (2012) is related to facial development. As expected, the discriminant power of this approach did not reach optimal effectiveness in the Brazilian sample. In practice, this age estimation method must be adapted for more reliable application in Brazilians. [ABSTRACT FROM AUTHOR]

Conservation of the three-dimensional aspect of bitemarks is important to optimize their correct analysis. Bitemarks could be recorded with a surface scanner. In clinical dentistry, optical cameras are used as an alternative to conventional dental impressions. They scan the dental relief for prosthetic design. We propose a three-dimensional bitemark analysis protocol using an intraoral scanner. Eight volunteers bit on different materials: dental wax and hard cheese. Then, bitemarks and dentitions were scanned with the intraoral scanner. The scans obtained were exported into the mesh comparison software, CloudCompare®, which can automatically generate overlays. For this purpose, dentition were aligned and compared with indentations. The meshing and non-meshing areas were easily visualized using a colorimetric scale. This protocol made it possible to exclude incorrect dentitions in about twenty minutes. Moreover, dentitions corresponding to bitemarks all matched. In order to refine this protocol, it must be tested with morphologically close dentitions and on the skin as well. [ABSTRACT FROM AUTHOR]

Bone is a hierarchical composite material composed primarily of collagen molecules, mineral crystals, and water. The mineral phase confers strength and stiffness while the organic matrix provides toughness. As a result, living bone is very capable of absorbing energy and resisting fracture. After death, the bone often becomes dehydrated and the collagen degrades causing greater stiffness and reduced toughness. These changes in mechanical properties are augmented due to the combined effects of water loss and collagen degradation. As a result, bone becomes more brittle postmortem, which causes the changes in fracture characteristics that are commonly used to estimate the timing of the fracture. However, because the loss of moisture and collagen degradation are influenced by taphonomic conditions, anthropologist must use caution when interpreting the timing of fractures based solely on fracture characteristics. As part of this special volume on hard tissue alterations associated with trauma, the aim of this article is to provide an overview of the mechanical changes that occur in bone postmortem and summarize major works in bone biology and anthropology examining the cause and timing of plasticity loss in bone after death. [ABSTRACT FROM AUTHOR]

The relationship between the point of blunt impact and the location of cranial fracture initiation continues to be poorly understood. The current study used high-speed video to capture cranial fracture initiation and propagation in impact experiments on twelve unembalmed, intact human cadaver heads. Video footage provided direct evidence that blunt cranial impacts can produce linear fractures initiating peripheral to the impact site. Four tests produced only remote peripheral linear fractures with no damage at the known point of impact, demonstrating that the pattern of linear fractures does not necessarily indicate impact site. The range of variation observed in these experiments suggests that cranial fracture formation is more complex than it is typically described in the current literature. Differences in biomechanical and fracture results obtained with three different shaped implements provided evidence that impact surface is one important factor influencing the outcomes of blunt cranial impacts. [ABSTRACT FROM AUTHOR]

Dental and maxillofacial trauma analysis in the forensic context, includes assessment of both living and deceased persons. It is required in all cases where human abuse is suspected and where signs of skeletal trauma are detected in human remains. Skeletal trauma refers to the damage inflicted to bone, teeth and other hard tissues. An accurate analysis of the dental and maxillofacial trauma is achieved by the assessment of the remains by forensic pathologists, anthropologists and odontologists. This paper aims to review dental and maxillofacial skeletal trauma in the scope of forensic science. High-velocity projectile, sharp force, blunt force, and thermal trauma showing different traits in maxillofacial structures are discussed. Regarding non-fatal physical abuse, head and neck are the most frequently injured areas, resulting in fractures, contusions, burns and traumatic dental injuries. [ABSTRACT FROM AUTHOR]

Sharp force trauma (SFT) in bone and cartilage has been studied extensively. This literature review summarizes knife and saw mark research. Researchers have documented several features of cut surfaces and successfully associated them with various tool characteristics. Most study designs are based on light microscopic examination, but other technologies such as micro-computed scanning, scanning electron microscope, and epifluorescence microscopy have been investigated. Researchers have worked with human and non-human material, and found that the presentation of SFT differs between the two. Furthermore, they have designed studies to control the parameters surrounding SFT (e.g., tool angle, force, direction) as well as not to control these parameters (real-world scenario) and have found that the trauma produced in the two scenarios differ considerably. Researchers have attempted to calculate the error rate associated with cut and saw mark analysis and have reported very different results. Several high profile cases of successful SFT analysis have been published and are briefly reviewed. Expert testimony based on cut and saw mark analysis has been found admissible, but not in all cases. Unfortunately, researchers have not consistently used standard terminology, a list of terms gathered from the literature is provided. Despite the extensive research, more work is needed. Methods that mitigate potential sources of error that are not dependent on analyst's experience must be developed. [ABSTRACT FROM AUTHOR]

Blunt traumas are the most common injuries observed and reported in medical examiner settings. Two common methods to describe bending bone fractures in the anthropological literature include the application of morphology nomenclature and describing characteristic fracture morphology. A nomenclature descriptor of blunt trauma, the butterfly fracture, is commonly used to describe broken long bones. In this paper, a case study of a fractured long bone in a pedestrian vehicle accident is used to highlight the complex interplay of factors involved in bone fracture formation. The application of a butterfly fracture pattern in trauma analysis is useful in establishing the bending direction of a bone, in identifying failure modes, and is valuable in teaching. Yet, butterfly fracture characteristics need to be examined in 3-dimensions for diagnosis of modes of failure, tension, shear and compression, and even then, the bending direction of a broken bone may not provide a reliable indicator of the point of impact (POI); this is especially true when a priori knowledge of the injury is unknown. Common fracture nomenclature, such as oblique, transverse and/or comminuted, as well as eponyms, are medical descriptions of an injury which are impractical to use for interpreting a broken bone from fleshed or skeletonized remains, in establishing a POI and in evaluating total bone trauma (TBT). The examination ofcharacteristic features on the surface of a broken bone associated with the modes of failure is the best approach for establishing the bending direction of a long bone. [ABSTRACT FROM AUTHOR]

Ancestry estimation from skeletal remains is a challenging task, but essential for the creation of a complete biological profile. As such, the study of human variation between populations is important for the fields of biological and forensic anthropology, as well as medicine. Cranial and dental morphological variation have traditionally been linked to geographic affinity resulting in several methods of ancestry estimation, while the postcranial skeleton has been systematically neglected. The current study explores metric variation of the tibia in six Mediterranean populations and its validity in estimating ancestry in the Mediterranean. The study sample includes 909 individuals (470 males and 439 females) from Cyprus, Greece, Italy, Spain, Portugal and Turkey. The sample was divided in two subsamples: a reference and a validation sample. Multinomial regression models were created based on the reference sample and then applied to validation sample. The first model used three variables and resulted in 57% and 56% accuracy for the two samples respectively, while the second model (6 variables) resulted in 80% and 74% respectively. Classification between groups ranged from 28% to 95% for the reference sample and from 15% to 91% for the validation sample. The highest classification accuracy was noted for the Greek sample (95% and 90% for the reference and validation sample respectively), followed by the Turkish sample (74% and 78% respectively). The Spanish, Portuguese and Italian samples presented greater morphological overlap which resulted in lower classification accuracies. The results indicate that although the tibia presents considerable variation amongst neighbour populations it is not suitable as a sole skeletal element to separate all groups successfully. A combination of different skeletal elements may be required in order to achieve the levels of reliability required for forensic applications. [ABSTRACT FROM AUTHOR]

In forensic anthropology, the estimation of age at death is mainly required to establish a biological profile and facilitate individual identification. The Suchey-Brooks (SB) system represents one of the most commonly used and tested methods of assessment based on the morphological characteristics of the pubic symphysis. However, this method has certain drawbacks, including frequently inaccurate estimation for older individuals. The aim of this work was to test the evolution of pubic bone mineral density (BMD) according to age, especially for individuals over 40 years old. We retrospectively studied pubic bones from males (n = 88) and females (n = 113) over 40 years of age undergoing clinical multi-slice computed tomography (MSCT) in a French hospital between November 2017 and April 2018. The results revealed a significant negative correlation between BMD and age for males (R = -0.62) and females (R = -0.55). The intra- and inter-observer reliabilities of the phase allocation were moderate for males (kappa values at 0.72 and 0.65) and strong for females (kappa values at 0.93 and 0.86). Moreover, a significant difference in BMD between stages 6-1 and 6-2 for males was observed. We hypothesize that BMD might help improve the reliability of the SB system for older individuals. In addition to the pubic bone, numerous other anatomical regions such as the area of Ward could represent interesting areas of study in order to relate bone density to age. [ABSTRACT FROM AUTHOR]

Traumatic diastasis of cranial sutures is a type of bone fracture more common in children than in adults, but little attention has been paid to this skull damage. Differentiation between inflicted and accidental traumatic head injury is still a challenge in forensic pathology, particularly in pediatric population. In fact, diastasis of cranial sutures may occur with or without other skull fractures and may be the only evidence of an abusive head trauma (AHT). This is a case study dealing with undetected traumatic diastasis of cranial sutures in child abuse. The skeletonized juvenile remains were found inside a suitcase. A diastasis of the coronal and sagittal sutures was the only finding recorded at the autopsy with no other relevant bone defects. The diastasis was originally attributed by the medical examiner to a physiological unfused stage of the calvarial bones. Therefore, the cause of death was undetermined. Twelve years later an anthropological revision of the cold case showed that diastasis of the coronal and sagittal sutures was assessed as the evidence of an AHT. Analysis of skull fractures in child abuse can be challenging as normal skull suture variants mimicking intentional injury are reported. Diastasis of the cranial sutures can be also a post-mortem effect of burning or freezing. Therefore, a differential diagnosis between natural, accidental or inflicted skull defects is mandatory in death investigation. A multidisciplinary approach in such circumstances is strongly recommended in order to reduce the risk of misdiagnosis. [ABSTRACT FROM AUTHOR]

Human bone histological analysis is a useful tool to assess post mortem diagenesis and to predict successful nuclear DNA typing of forensic material. This study is part of a series of studies developed by the authors intended to improve the understanding of post mortem diagenesis and to develop applications for DNA analysis of skeletal species from tropical soils, in order to optimize genetic and anthropological protocols. The aim of this study was to analyze the impact of burial period on the integrity of exhumed compact bone microstructure from tropical climate. In fragments of exhumed human femora from 39 individuals from the same cemetery (exhumed group) and 5 fresh femora from routine autopsies (control group), sections stained by hematoxylin-eosin were analyzed in order to measure bone microstructural integrity. We found that bone integrity index in exhumed group was negatively influenced by the period of burial (r = -0.37, p < 0.05) and highly significantly decreased (p < 0.0001) in comparison to control group. The period of burial and nitric acid decalcification time was positively correlated (r = 0.51; p < 0.01), leading to imply a bone petrification process during inhumation. Exhumed group showed higher level of matrix bone loss (p < 0.001), as expected, and 87% of cases analyzed were "tunneled" as described by Hackett. Bone integrity index and bone matrix tend to decrease in bones buried in tropical soil between 8-14 years of inhumation. This period is short if we consider cases in which there are preserved bones interred for longer periods in other environments. These data must be considered in cases where genetic identification of exhumed skeletons from tropical environment is required. The diagenesis in these bones and the variations of results found are discussed, clarifying some challenges for forensic laboratories, especially in DNA analysis. [ABSTRACT FROM AUTHOR]

The absence or poor preservation of vertebrae often prevent the application of the anatomical method for stature estimation. The main objective of this paper was to develop a web app based on artificial neural network (ANN) models to estimate the vertebral height of absent or poorly preserved vertebrae from other vertebrae and thus enable the application of anatomical methods. Artificial neural models were developed based on the vertebral height of vertebrae C2 to S1 of a sample composed of 56 adult male and 69 adult female individuals. The skeletons belong to the Identified Skeletal Collection of the University of Coimbra and the ages at death of these individuals ranged from 22 to 58 years old. Statistical analysis and algorithmic development were performed with the R language, R Core Team (2018). Intra- and inter-observer errors regarding the vertebral height were small for all vertebrae (<0.45 mm). Significant models to estimate vertebral height were obtained for both sexes and for the sex-pooled group, although none with an R2 higher than 0.48 and 0.34 for the C2 and the S1, respectively. The root mean square error (RMSE) regarding the predicted vertebral height and the observed vertebral height was almost always smaller than 1.0 mm while most R2 values were higher than 0.6 although models with worse performances were obtained for some vertebrae located at the ends of the vertebral column (C3, L4, and L5). The ANN models have clear potential to predict vertebral height. This mathematical approach may be used to enable the application of the anatomical method for stature estimation when some vertebrae are absent or poorly preserved. The application of the ANN models can be carried out by using the new web based app SPINNE. [ABSTRACT FROM AUTHOR]

Assessing sex and population affinity is an important part of the process of biologically identifying unknown human remains, and the skull is usually one of the best structures for assessing both these components of the biological profile. Population affinity is known to be a hugely important variable when estimating sex because the manifestation of sexually dimorphic traits, body size or social and behavioural habits differs across populations. Therefore, for forensic purposes, the estimation of ancestry is a necessary step in the identification of bone remains. The present study improves on the results of a previously developed virtual method using the exocranial surface for sex estimation and assessing population affinity. The ability to assess these components of the biological profile was successfully tested on 208 individuals from two recent European populations. The original classifier was based on geometric morphometric analyses (CPD-DCA, PCA, SVM) and was able to assess the sex of individuals belonging to one French population with an accuracy exceeding 90 % Musilová et al. [1]. To improve the reliability of the method, the Czech population sample was added to the dataset, yielding the highest accuracy of 96.2 %; using the combined dataset, the reliability of the method was 91.8 %. Secondly, we used the same method utilizing inter-population differences to classify individuals based on the shape of the skull. The greatest accuracy rate was 92.8 %, which makes our method a promising tool for sex estimation and assessing population affinity. [ABSTRACT FROM AUTHOR]

The diaphyseal cross-sectional geometric properties of the humerus, femur and tibia have been extensively used for studying their adaptation to mechanical loading. To date common practices for such studies involve either computed tomography or the latex cast method in conjunction with image analysis for calculating such properties. With the advent of modern laser scanning and photogrammetry technologies in biological anthropology, the computation of the cross-sectional geometric properties directly from 3D models is a viable and sensible alternative. Nevertheless, such method has not been properly implemented as yet. A dedicated toolkit, named long-bone-diaphyseal-CSG-Toolkit, comprising a set of functions for the GNU Octave programming language, is presented here. Offering a robust analytical implementation and an easy to follow application either for a single bone or in batch-processing mode, the toolkit requires minimum user intervention and also provides functionality for graphical representation of the calculated periosteal contours and their respective cross-sectional geometric properties. Finally, the long-bone-diaphyseal-CSG-Toolkit utilizes advanced optimization algorithms, which eliminate intra- and inter-observer error by reliably orienting the cross-sectional contours to a well-defined orientation and close to the bone's true anatomical position, which provides a significant advantage over the latex cast method. [ABSTRACT FROM AUTHOR]

The purpose of this study is to assess the relevance of computational anatomy for the sex determination in forensic anthropology. A novel groupwise registration algorithm is used, based on keypoint extraction, able to register several hundred full body images in a common space. Experiments were conducted on 83 CT scanners of living individuals from the public VISCERAL database. In our experiments, we first verified that the well-known criteria for sex discrimination on the hip-bone were well preserved in mean images. In a second experiment, we have tested semi-automatic positioning of anatomical landmarks to measure the relevance of groupwise registration for future research. We applied the Probabilistic Sex Diagnosis tool on the predicted landmarks. This resulted in 62% of correct sex determinations, 37% of undetermined cases, and 1% of errors. The main limiting factors are the population sample size and the lack of precision for the initial manual positioning of the landmarks in the mean image. We also give insights on future works for robust and fully automatic sex determination. [ABSTRACT FROM AUTHOR]

Age estimation in growing dogs is crucial not only in clinical practice but increasingly so in forensic practice as well. In the last few years, it has assumed great importance for correctly identifying the age of puppies illegally imported to Italy as well as to other European countries. Puppies are, in fact, transported when they are too young to be moved, which can cause both animal/public health and animal welfare issues. Therefore, the movement of animals within the European Community is governed by strict rules, and veterinarians are often required to evaluate the age of the imported puppies in a forensic scenario as accurately as possible. To date, X-ray evaluation of limb bones ossification centers (OCs) is generally accepted as a valid tool to assess the age of puppies. A wealth of information exists on this topic but it is not always easily available. This work is a historical review of the existing literature and proposes two tables illustrating the timelines of limb OCs appearance and closure, coming to terms with forensic requests to evaluate the age of a puppy. The timelines reported indicate the need to improve methodology to enhance the accuracy and to reduce the error in age estimation. [ABSTRACT FROM AUTHOR]

A primary concern in forensic anthropology, when reconstructing the biological profile of an unidentified individual is ancestry and sex estimation. The development of multivariate statistical methods and the assembly of large reference sample databases gave rise to the development of specialized computer software for sex and ancestry estimation. Among various such software, the 3D-ID is the only freely-available program that can handle missing values in the input dataset. The present study evaluates the reliability of 3D-ID in correctly classifying ancestry and sex of 158 test subjects from the Athens Collection, a documented Greek population sample. 3D-ID's classification performance was evaluated both separately and collectively for sex and ancestry. According to our results, the accuracy regarding sex estimation ranged from 74.05% to 86.7% for cases with unknown ancestry and reached 89.87% when testing within the Southeastern European reference group, whereas ancestry estimation accuracy reached 70.9% for correctly classifying the Greek individuals to European population groups. We conclude that 3D-ID software exhibits moderate reliability in ancestry estimation and adequate reliability in sex estimation. The Greek population seems to deviate from the 3D-ID software's reference samples and therefore caution should be taken in interpreting 3D-ID's results of unknown subjects, for which the software's reference sample database may not be representative. 3D-ID's guidelines for using 19-landmark configuration improves the accuracy of ancestry estimation and form variables should be preferred for sexing samples. [ABSTRACT FROM AUTHOR]

The use of bone mineral density (BMD) to predict age-at-death in skeletal remains provides a usable alternative to other methods because the values obtained are not observer-dependent. The aim of this study was to investigate the usability of BMD to estimate age in South African populations, and to assess inter-population variation and sex-specific differences in BMD values from the proximal end of the femur. In order to estimate age, regression analysis was done for the construction of population dependent formulae. The sample comprised of a total of 123 femora of black and white South Africans. DXA scans were performed using the Hologic Discovery system. Data analysis was done by employing independent-samples t-tests and correlation/regression analyses. The results indicated a statistically significant difference between black and white South Africans. Male groups were also significantly different from one another, but black and white females showed no significant differences. Linear regression analysis revealed a significant correlation between BMD values and age for the white population and the combined sample, but not for the black population. Bootstrapping were employed to confirm validity of the results. In conclusion, this study showed that the use of DXA measurements of the femur for estimating age may be used for the estimation of age-at-death in white South Africans, but more research is needed to better understand the relationship between bone mineral density and age in black South Africans. [ABSTRACT FROM AUTHOR]

Age-at-death estimation in a skeletal assemblage (target sample) is biased by the demographic profile of the material used for age prediction (training sample) when this profile is different from that of the target sample. This bias is minimized if the demographic profile of the target sample is properly taken into account in the method developed for age-at-death estimation. In the Bayesian approach this is accomplished via the informative prior. For methods based on regression, we propose two techniques: (a) using weighting factors taken from the demographic profile of the target sample, and (b) creating a new hypothetical training sample that has a demographic profile similar to that of the target sample. The two techniques, as well as the Bayesian approach, were tested using 532 artificial systems in which the age marker exhibited an eight-grade expression. It was found that depending on the criteria used for evaluation, the proposed approaches and especially the one based on a hypothetical training sample, may give better results than the Bayesian method in more than 90% of the systems studied. A basic prerequisite for the good performance of the proposed approaches is to select carefully the training sample. This sample should exhibit a uniform demographic profile or a profile with almost equal numbers of young and older individuals. All the above hold if the training and the target samples have different demographic profiles. If the profiles are the same or very similar, the best aging method is the direct regression using simple linear models. [ABSTRACT FROM AUTHOR]

This case study reports the anthropological analysis of bones remains discovered on Riou Island (Marseille, France) and the story of two World War II fighter pilots. The discovery of bones on "The Fountain of the Greeks" square on Riou Island occurred in the 1960's and a first anthropological study described a 35-year-old man, about 1.77 m tall, buried since an estimated period between the 13th and 16th centuries. The case was "closed" and the bones were considered as isolated archaeological remains. Few years later, near the coasts of Riou Island, parts of two planes were discovered. One was from of a German Messerschmitt Bf 109 F-4 of the Luftwaffe piloted by Prince Alexis fürst zu Bentheim und Steinfurt, and the other from a French P-38 Lightning F-5 B piloted by Antoine de Saint-Exupery. Therefore, the identification of the skeletal remains mentioned above was then thought to be perhaps one of the two World War II pilots. In this particular context we performed forensic and molecular biology analyses to resolve this identification. [ABSTRACT FROM AUTHOR]

Human Taphonomy Facilities (HTFs) are outdoor laboratories where scientific research is carried out on donated human cadavers in order to understand how human decomposition progresses in a variety of conditions. There are currently eight such facilities in the USA, one in Australia and one on mainland Europe. Forensic scientists in the UK have started to ask the question 'Does the UK need a Human Taphonomy Facility?'. A review of the literature produced by the existing HTFs, as well as published opinion and commentaries about these facilities and the feasibility of one in the UK has been undertaken. The existing arguments for and against the establishment of a Human Taphonomy Facility in the UK have been examined. Given recent media interest in the possibility of the establishment of a Human Taphonomy Facility in the UK, and the surrounding controversy, it is important to evaluate the potential benefit or harm of the creation of such a facility to Society and the scientific community. [ABSTRACT FROM AUTHOR]

Histomorphometric methods for age-at-death estimation constitute alternatives to macroscopic ones in the forensic context. However, all new methods must be tested on independent samples to justify their use and assure their accuracy. The main goal of this study was to test a new age-at-death method presented by Goliath et al. (2016) on a sample of 29 decalcified femur sections from individuals autopsied in the Institute of Legal Medicine of Montpellier, France. The formula proposed by the authors was not efficient to estimate the age-at-death in our sample, with only four out of 29 individuals well estimated. Despite this result, the same relationship between age and histomorphometric criteria was found, though at a lower intensity than what Goliath et al. previously found in their study. The use of a different bone preparation technique from Goliath et al. does not allow us to propose a definitive conclusion on the accuracy of this method. [ABSTRACT FROM AUTHOR]

For medico-legal forensic practitioners the identification of unknown remains is an important part of any investigation, often predicated on having accurate estimations of age and sex. In considering the specific skeletal elements available to facilitate such biological information, the cranium is frequently targeted for analysis, as it exhibits marked traits of sexual dimorphism, and also has a predictable pattern of growth. There are, however, instances where it may not be possible to estimate skeletal sex, especially in the juvenile skeleton. There is still considerable uncertainty surrounding the age at which the human cranium is quantifiably dimorphic. The aim of the present study is to explore age and sex variation in three-dimensionally reconstructed MDCT scans of the juvenile cranium. The study sample comprises 152 juvenile crania from a Western Australian population; a total of 52 three-dimensional landmarks are acquired and analyzed using Procrustean geometric morphometrics. Group discrimination is assessed between sexes and across age classes. Results demonstrate that sexual dimorphism and age variation is discernible through geometric morphometric analysis of form, size and shape. Relative to sex and age, size is found to be generally equivalent to, or even more accurate than, shape data. There is little quantifiable sexual dimorphism in individuals younger than 12years of age with most variation related to age; discrimination improves with increasing age, with average hit rate (HR) values increasing from just over 50% (52-58%) to more than 90% (93-94%) accuracy at 18years. In contrast, differences between contiguous age classes follow the opposite trend and tend to be larger in prepubertal groups, while becoming progressively smaller in older age classes. This study demonstrates that simple linear interlandmark distances describing overall cranial size may provide a simple option for preliminary classifications of age and sex in skeletal remains of forensic interest. However, although recombining size and shape to perform analyses using form generally does not appreciably improve predictive accuracy, it potentially contributes to increased confidence in group assessment (especially for sex) and thus offers a promising, albeit complex, type of information to discriminate groups based on cranial size and/or shape. [ABSTRACT FROM AUTHOR]

Morphological changes on the surface of the pelvic bone can be used to estimate the age at death of a person. These features can be visualized using three dimensional computed tomography (3D CT) images. A newly introduced 3D CT technique, cinematic volume rendering, improves visualization of the surface of bones by integrating the effect of light on the images. The aim of this study is to evaluate the efficacy of this 3D CT technique in visualizing features related to age estimation in pelvic bones. Dry pelvic bones of 35 subjects were scanned and then 3D reconstruction of the images was performed using the cinematic rendering technique. The 3D CT images of the pubic symphyses and the auricular surfaces were interpreted by two radiologists and a forensic osteologist using age estimation features derived from the Suchey-Brooks and the Buckberry-Chamberlain methods The interpretation of the dry pelvic bones was done by an expert anatomist and used as a gold standard. The percentages of correct interpretations and level of agreement in grading using 3D CT and using dry bones were high for features in the pubic symphyses including surface patterns (100%, k=1), presence of lower extremities (100%, k=1), and patterns of pubic rims (91.4%, range 87.5-100%, k=0.88). In the auricular surface of the ilium, all specimens with an apical activity were correctly interpreted (100%, k=1), but detection was moderate to poor for transverse organization (71.4%, k=0.43), macroporosity (70%, k=0.38.), and microporosity (52.9%, k=0.25). Cinematic volume rendering has a high level of efficacy in identifying age-related features on pubic symphyses, but it inadequately displays features on the auricular surface. [ABSTRACT FROM AUTHOR]

The objective of this project was to document the efficacy of part-to-part comparison of computed tomography (CT)-derived three-dimensional (3D) models of the lumbar spine in forensic personal identification. By testing the methodology, this study aimed to provide a new technique of quantifiable (through a percent match) positive identification that meets the explicit requirements of the Daubert ruling and the challenges set forth in the 2009 NAS report. Ante-mortem (AM) and simulated post-mortem (PM) models of the lumbar vertebrae (L1-L5) for 30 unique individuals were compared via part comparison analyses. The threshold of ±0.5mm with at least a 90% match was considered a positive identification. Using this threshold, the part comparison results had a perfect identification rate with no false positives and no false negative matches. A ROC curve was generated with a score of 1, signifying a "perfect" sensitivity and specificity, at a cut-off value of 65.5%. On average positive IDs had a 94.7% percent match within the established threshold, while negative IDs had an average of 21.4%. In looking at the impact of different components of the biological profile, age and sex of the unknown individual played a minimal role in the percent match for both a positive and a negative ID. Lumbar level also played a minor role in in both the positive and negative percent match. The real-world application of 3D part-to-part comparison on AM and simulated PM scans demonstrate the potential usefulness of this technology in forensic identification. [ABSTRACT FROM AUTHOR]