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Grape syrup (Pekmez or Dooshab) is one of the nutritious products developed through grape processing. One of the main challenges in the industrial manufacture of this product is the utilization of traditional pekmez earth for tartaric acid adsorption. The objectives of this study were to investigate the effect of calcium carbonate, nano-silica, alumina, and activated carbon as adsorbents and also contact time in grape juice deacidification, and to determine the effects of these adsorbents on the physicochemical properties of grape juice by using the Box-Behnken statistical design. By applying different amounts of these adsorbents in grape juice, the magnitude of acidity decrement and the physicochemical properties such as acidity, pH, transmittance, the amount of reducing sugars, formalin index, and adsorption efficiency were investigated. Data analysis showed that different mixtures of adsorbents at different concentrations had significant effects on acidity and pH of the samples but no effects on the level of reducing sugars and formalin index were observed ( p  > .05). According to the results, the adsorption capacity with the highest calcium carbonate content (0.7 g/100 ml) was about 88%; the maximum acidity decrements of up to 92% were achieved using the treatments containing calcium carbonate, nano-silica, and activated carbon, while alumina failed to affect the acidity of the samples. Optimum conditions were obtained in 1.27, 0.21, 0.7, and 0.07 g/100 ml alumina, nano-silica, calcium carbonate, and activated carbon, respectively, resulting pH 4.3 and acidity 0.37% in grape syrup., Competing Interests: The authors declare that they do not have any conflict of interest., (© 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.)