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In this study, Mediterranean mussels (Mytilus galloprovincialis) coming from Italian production were purchased in the most important Italian wholesale fish market in different seasons. Biometrical parameters and chemical composition were investigated, with a particular focus on lipid quality and fatty acids (FAs) composition. Results showed a valuable nutritional profile independently by the season of production, represented by high protein and low-fat content, with the lipid portion represented by high amounts of beneficial FAs, particularly the long chain of the n-3 series. Some differences (p < 0.05) were found in carbohydrates and fat content of mussels edible tissues and in FAs profile of specimens collected in different seasons. The most favourable composition in terms of lipid quality was found in mussels collected during spring, corresponding to the moment of the year when mussels store energy reserves in the form of carbohydrates and fat (preparing for the spawning events) and when seawater is enriched in phytoplankton. The lipid health indices calculated (n6/n3, AI, TI) showed optimal values independently by the season of production. The outcomes obtained in this study could help supporting the appreciation of Mediterranean mussels as nutritional valuable seafood product, thus helping encouraging their consumption and promoting the appraisal of this farming sector essential for Italian aquaculture and related to low environmental impact. [ABSTRACT FROM AUTHOR]

Current study aims to evaluate the effect of essential oil on the conservation of chicken fillets. Following a screening, Tetraclinis articulata was selected for its antimicrobial (Enterococcus feacalis ATCC 29212, MIC < 0.031 mg/mL) and antioxidant (IC50 = 1000 µg/mL) potentials with no cytotoxicity effect towards murine macrophage cells. The treatment of chicken fillets, especially with 200 ppm of T. articulata essential oil per 100 g of product reduced significantly (p < 0.05) lipid oxidation during 12 days of refrigerated storage. Microbial flora charges decreased significantly (p < 0.05) with a rate of 50.31% for 200 ppm of essential oil from the 6th day of storage. The acidity of treated fillets was lower than control samples (1.3 g/kg) from the 3rd day of storage. In addition, a clear effect was recorded by reducing water losses during cooking under the tested doses of essential oil. Withall, findings encouraged the use of T. articulata to extend poultry meat product shelf life. [ABSTRACT FROM AUTHOR]

The effectiveness of commercial oxygen scavengers was investigated in order to increase the shelf-life of sliced dry-cured Iberian shoulder in MAP (modified atmosphere packaging) for up to 150 days. Five dry-cured shoulders from Iberian pigs were used. Slices of these dry-cured shoulders were randomly packaged in MAP conditions. An active packaging (AP) with oxygen scavengers was evaluated to reduce the level of oxygen within the headspace as close to 0% as possible. AP was compared to a Control Treatment (C) (without scavenger). Sliced dry-cured Iberian shoulder in AP showed lower thiobarbituric acid reactive substances values (TBARS) than control packages after 150 days of storage, and in general, volatile compounds derived from lipid oxidation, increased in C packages, whereas these remained steady in AP. Therefore, AP was effective to decrease the development of lipid oxidation during storage. In contrast, AP was not effective in preserving color changes, although no sensory differences between treatments were appreciated by the panelists. [ABSTRACT FROM AUTHOR]

A growing market for seaweed products has emerged in the last 20 years, with microalgae and their extracts representing a source of high-value chemicals. In this study, antioxidative and anti-inflammatory activities of lipid fraction extract from Chlorella vulgaris (Chlorella) and Arthrospira platensis (Spirulina) on Normal Human Dermal Fibroblasts (NHDF cells) were evaluated. Treatments with Chlorella and Spirulina lipid extracts in NHDF cells at 0.1, 0.5, and 1% significantly protect UVA-induced damage by decreasing oxidative stress in the form of reactive oxygen species (ROS) but do not display any significant protection from HEV-induced damage. Chlorella or Spirulina lipid extracts at 0.1 and 0.5% display significant anti-inflammatory effects by inhibiting the 2-propanol-induced IL-6 expression on NHDF cells in vitro but showed no decrease in TNF-α expression. Chlorella and Spirulina lipid extracts present antioxidant and anti-inflammatory activity on NHDF cells. [ABSTRACT FROM AUTHOR]

Fish is rich in polyunsaturated fatty acids with beneficial effects on human health; however, these lipids are very sensitive to auto-oxidation reactions, leading to loss of nutritional and sensory quality. The effect of traditional (brining, smoking) and novel (addition of polyphenolic extracts, high pressure) preservation processes on the antioxidant/oxidative status of muscle lipids from dolphinfish and sardine was studied. Brining with oregano or rosemary aqueous extracts, as well as smoking, gave rise to deposition of phenolic compounds (9–42, 1.5–4.5 and 0.4–2.3 µg phenol/g for smoked, oregano-brined and rosemary-brined samples, respectively) in the muscle of both fish species. The antioxidant activity, as measured by ferric reducing ability, was also improved after brining with antioxidant extracts or smoking, results ranging from 8.9 to 82 mM FeSO4 · 7H2O equivalents/mg muscle depending on the treatment and the fish species. Consequently, fish lipid oxidation (as measured by thiobarbituric acid reactive substances) derived from brining and pressurizing, applied alone or in combination, was reduced between 6.6 and 69.8% depending on the treatment and the fish species. The combination of brining with oregano extract and light smoking showed an additional antioxidant effect, as compared with that obtained by smoking, on reducing sardine lipid oxidation derived from brining and pressurizing. [ABSTRACT FROM AUTHOR]

Antioxidant activity of brown lead (Leucaena leucocephala) seed extracts with and without prior chlorophyll removal was studied in comparison with mimosine. Both extracts showed similar hydroxyl radical (HO•) scavenging activity, hydrogen peroxide (H2O2) scavenging activity, singlet oxygen inhibition and hypochlorous acid (HOCl) scavenging capacity (p > 0.05). Nevertheless, the extract without prior chlorophyll removal had higher oxygen radical absorbance capacity than that with prior chlorophyll removal (p < 0.05). Generally, lead seed extracts with and without prior chlorophyll removal possessed a lower antioxidant activity, compared with mimosine. When lead seed extract without prior chlorophyll removal (100 and 200 ppm) was used in different lipid oxidation model systems, including β-carotene-linoleic acid and lecithin liposome systems, the preventive effect toward lipid oxidation was dose-dependent. At the same level of use, mimosine exhibited a higher efficacy in prevention of lipid oxidation in both systems as indicated by the lower increases in thiobarbituric acid reactive substances. A similar result was obtained in minced mackerel. Therefore, lead seed extract containing mimosine could act as a natural antioxidant to prevent lipid oxidation in foods. [ABSTRACT FROM AUTHOR]

The study was designed to investigate the fatty acid composition in longissimus dorsi (LD) and semimembranosus muscles (SM) and subcutaneous tissue of hybrid (1/4 Lithuanian indigenous wattle, 1/4 wild boar and 1/2 Yorkshire or 1/2 Landrace) pigs. The SM showed a tendency to have lower contents of monounsaturated fatty acids (MUFA) and higher contents of polyunsaturated fatty acids (PUFA) than the LD muscle. No statistically significant differences were observed between the muscles in lipid quality indices, i.e., atherogenic (AI) and thrombogenix (TI) indices and the ratio of hypocholesterolemic and hypercholesterolemic fatty acids (h/H). In the SM, the content of PUFA from Yorkshire-sired hybrids was relatively 47.8% higher, including C18:2n-6, C20:4n-6 and DPA (C22:5n-3) that were higher, respectively, by 44.3%, 86.6% and 51.6% in comparison with those from Landrace-sired hybrids. The gilts had a lower content of MUFA, including C18:1 and C20:1n-9 fatty acids in the LD muscle and tended to have a higher content of PUFA in the SM compared to the barrows. Neither the terminal breed in crossing, nor the gender had any significant effects on the proportions of total saturated, MUFA and PUFA in the subcutaneous tissue. [ABSTRACT FROM PUBLISHER]

Lipid damage of farmed spotted Babylon snail muscle stored in ice for 7 days was evaluated by determining changes of lipid composition, fatty acid composition, peroxide value (PV), and p-anisidine value (p-AV). During 7-day iced storage, triglyceride, and phospholipids contents decreased but free fatty acid increased with storage time (p≤0.05). Changes in saturated and polyunsaturated fatty acid were observed at extended storage time (p≤0.05). No changes in PV and p-AV of snail muscle were found during the first 4-day iced storage (p>0.05). However, PV and p-AV of snail lipid increased during 4-day and 7-day iced storage (p≤0.05). Rancid odor assessment of snail muscle exhibited a significant increase in rancidity (p≤0.05) after 3 days of storage. Bacteria counts of snail muscle exceeded 7 log CFU/g, which was considered as the limit for acceptability after 7 days of iced storage. In conclusion, these results revealed that lipid damaged by hydrolysis and oxidation occurred in snail muscle during iced storage. [ABSTRACT FROM AUTHOR]

This study investigated the effect of lipid types (oleic acid, palm oil, and margarine) and their concentrations (0, 10, 20, 30, 40, and 50 wt%) on the water vapor permeability (WVP), tensile strength (TS), percentage of elongation at break (%E), and structure of emulsified rice starch-chitosan composite film. The influence of rice starch-chitosan composite film incorporated with lipids in controlling the moisture transfer in moisture-sensitive products was determined by wrapping a low aw-type cracker product in rice starch-chitosan composite film. TS and WVP of rice starch-chitosan composite film decreased with the addition of lipids, whereas %E increased in these films. Addition of lipids significantly raised film yellowness for composite films. The results showed that films added with oleic acid gave higher L*, b*, and chroma values but lower a* value than margarine and palm oil, respectively. The lower transparency of the films was noticed when a greater amount of lipid was incorporated (p<0.05). Oleic acid-incorporated films provided the films with smoother surface and higher values of TS and %E but lower WVP than margarine and palm oil, respectively. Wrapping cracker samples in the rice starch-chitosan composite film incorporated with oleic acid could maintain the hardness and provide longer shelf life and lower moisture content than synthetic polyvinyl chloride film (PVC) and unwrapped (reference) crackers, respectively. [ABSTRACT FROM AUTHOR]

The objective of this study was to investigate the effect of different blended vegetable oils having different levels and profiles of polyunsaturated fatty acids (PUFA) on hypercholesterolemia by analyzing the changes in lipid profile in high-cholesterol diet fed rats. Three vegetable oils (soy oil, sunflower oil, and the nonconventional flaxseed oil) were blended to obtain blends rich in PUFA. Thirty albino rats were used over the 2-month period. The animals were divided into five groups, wherein group 5 represents negative control, where rats were fed basal diet, while rats in group 4 received high cholesterol diet and served as positive controls. The other three groups were fed hypercholesterolemic diet (1% cholesterol + 0.25% colic acid) supplemented with blended oils. Generally, rats fed blended oils showed significantly lower levels of total cholesterol (TC), triacylglycerol (TG), and low-density lipoprotein (LDL) cholesterol as well as higher levels of high-density lipoprotein (HDL) cholesterol, in comparison with animals fed high-cholesterol diet and cholesterol-free diet. Thus, oil blends under study may be useful formulations for the treatment of hypercholesterolemia. In addition to improving the lipid profile by lowering TC, total TG, and total LDL and increasing HDL, blending of vegetable oils can result in an economic advantage of lower prices. [ABSTRACT FROM AUTHOR]

The changes of the fatty acid (FA) profile of 2 muscles Longissimus dorsi and Biceps femoris from 3 Iberian × Duroc genotypes were studied: GEN1: ♂ Iberian × ♀ Duroc1, GEN2: ♂ Duroc1 × ♀ Iberian; GEN3: ♂ Duroc2 × ♀ Iberian. GEN1 and GEN2 are reciprocal crosses while the difference between GEN2 and GEN3 is the Duroc sire line. The genotype Duroci was selected for the production of dry-cured meat products while the genotype Duroc2 was selected for meat production. Longissiinus dorsi and Biceps femoris BF from the reciprocal cross showed similar changes in FAs profile after refrigerated storage. However, the Duroc sire line affected the FA profiles of intramuscular fat (IMF) and lipid fractions since some differences were found between GEN2 and GEN3. Meat from GEN3 had the highest level of polyunsaturated fatty acids (PUFA) in IMF and lipid fractions as well as the lowest rate of plasmalogens in polar lipid fraction. After storage, meat from GEN3 showed an increase of long chain PUFA in free fatty acids fraction and the highest increase in the ratio DMA/FA [(dimethylacetals/FAs) × 100] after the refrigerated storage, which was indicative of a higher deterioration of this genotype. Therefore, the crossbreeding of Iberian pigs with Duroc selected genotypes (Duroc2) could affect the changes in the FAs profile of meat under refrigerated storage. [ABSTRACT FROM AUTHOR]

Chitosan films prepared with oregano essential oil were applied on bologna slices. Release of the essential oil compounds during film preparation and application on the meat product and consumer accept- ability of bologna enriched with oregano essential oil were tested. Oregano essential oil compounds were quantified by gas chromatography mass spectroscopy (GCMS) after extraction from the film- forming solution, films before and after application on bologna and from bologna slices before and after application of the films. The results indicated that the concentration of components of the essential oil sharply decreased during film preparation, e.g. from 757.7 ppm carvacrol in film-forming solution to 2.1 ppm in dried films. No carvacrol was detected in the films after application on bologna for 5 days at 4°C, mainly due to its diffusion into bologna. It seemed that the moisture and high lipid content of bologna helped the diffusion of the oregano essential oil from the chitosan film matrix into the product. Sensory evaluation suggested that addition of 45 ppm or less of oregano oil to bologna would be accept- able to consumers. Results support the potential use of chitosan-oregano essential oil films as an antimicrobial packaging material for processed meat, [ABSTRACT FROM AUTHOR]

The aim of this work was to study the effect of two types of pig feeding systems (known as recebo) on fatty acid composition, tocopherols contents and susceptibility to lipid oxidation in muscle. A total of 26 lberian × Duroc (50%) barrows were divided into two groups (named as short replacement (SR) and long replacement (LR) groups) according to length of replacement of Free-range rearing (based on acorn and pasture) by an oleic acid and α-tocopherol (200 mg/kg) enriched concentrate feed (SR group: 67 days in free-range rearing and finished during 20 days with concentrate diet; LR: 55 days in Free-range rearing. finished 50 days with concentrate). No differences were found in chemical composition and colour of Gluteo biceps muscle between the two management systems, except for α-tocopherol content, which showed higher content (p < 0.001) in LR pigs than in SR pigs. The fatty acid composition of subcutaneous fat was slightly affected by feeding system. Total intramuscular and neutral lipids from Gluteo biceps muscle presented a higher proportion of C18:1 n - 9 (p < 0.05) in SR than in LR pigs; however, no differences were found in total saturated (SFA), monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA). Composition of polar Lipids revealed a higher proportion of MUFA and lower of total n - 3 fatty acids in LR than in SR pigs. Feeding regime significantly affected susceptibility of muscle to iron-ascorbate-induced peroxidation. Muscles from pigs reared on SR system showing higher levels (P<0.05) of MDA after 50 and 100 min of incubation. These results indicate that the content of oleic acid and α-tocopherol in muscle depends on the levels of both provided by dietary means, more than the practised handling. [ABSTRACT FROM AUTHOR]

The lipid peroxidation inhibitory effects of Hizikia fusiformis methanolic extract (HME) on fish oil and linoleic acid were studied by means of peroxide value (PV), thiobarbituric acid-reactive substances (TBARS), conjugated diene hydroperoxides (CDH) and weight gaining assays. Heat and UV light stability were determined by DPPH assay. HME significantly (p<0.05) reduced the lipid peroxidation in a dose-dependent manner. Increasing the level of HME from 0.01 to 0.1% caused a higher antioxidative effect than the one produced by butylated hydroxytoluene (BHT) (BHT reported the best effect compared to α-tocopherol and butylated hydroxyanisole). HME reduced the formation of primary oxidation products as shoed by the lower CDH values compared to its control counterpart. HME reduced also the addition of oxygen to form lipid peroxyl radicals that indicated a low weight gaining in HME-treated oils. Moreover, it could reduce the formation of lipid peroxide. Furthermore, it diminished the total lipid peroxidation resulting in low TBARS values. The heat and UV light study showed that H. fusiformis contained heat- and UV-light resistant antioxidants. These results indicated that the H. fusiformis antioxidants could be useful in preventing oxidative damages of food oils. [ABSTRACT FROM AUTHOR]

Proximate analysis of Brazil nut kernel showed 68.6 and 16.5% lipid and protein contents, respectively. The protein content in defatted kernel flour increased to about 46.7%. Extraction with 50 mM phosphate buffer containing 0.5 M NaCl (pH 7.2) solubilised about 81% proteins, out of which nearly 72% proteins (globulin) were recovered. Unextracted and soluble proteins accounted for 18.7 and 9.2% of total proteins, respectively. In relation to functional properties, the globulin showed pH of minimum solubility at 5.0. The globulin presented lower solubility (0.9-41.3%) in the pH range of 5.0-10.0 but above and below this pH range, there was a considerable improvement. The water and oil absorption capacities were 2.0 and 1.4 g/g globulin. The apparent viscosity of 1-3% globulin solutions at room temperature (29 °C) before and after heating at 90 °C for 15 min differed significantly. However, no significant difference was observed between apparent viscosity of unheated and heated globulin samples irrespective of their concentration. Emulsifying capacity varied with the pH, being maximum at pH 3.0 (94.5 mL/100 mg of globulin) and minimum (38.5 mL/100 mg globulin) at its isoelectric pH (5.0). Emulsion activity and stability also followed similar behaviour as that of emulsifying capacity. [ABSTRACT FROM AUTHOR]

Determines the lipid binding of fresh and stored formulated wheat breads. Incorporation of sodium stearoyl lactylate into dough formulation; Reactivity of lipids with amylopectin; Composition of lipids in breads.

The effects of different cooking techniques on the lipids of pork meat were evaluated. The assayed cooking techniques were deep-frying (DF), shallow-frying (SF), roasting (RO) and boiling (BO). Modi fication of the roasting meat due to reheating in a microwave oven after 24 h at 4 °C (MW), was also studied. In DF and SF significant increases were found in the lipid content (13.7 and 16.7 g/100 g of dry basis, respectively) and in the nun-polar lipids (11.2 and 14.2 g/100 g of dry basis, respectively) due to the absorption of the oil used in these cooking treatments. The main variations in the fatty acid composition of phospholipids and non-polar lipids were observed in the polyunsaturated fatty acid fraction. There was an increase in the content of free fatty acids (SFA, MUFA, PUFA) after MW, BO and RO. By contrast, free fatty acid content in DF and SF was similar to the content of initial raw meat. MW showed the greatest degradation of the phospholipid fraction, with a significant decline in phosphatidyl ethanolamine. All cooking treatments produced an increase in lipid oxidation, this be ing higher in RO and MW treatments. [ABSTRACT FROM PUBLISHER]

Main spectrophotometric and chromatographic methods for determination of lipid oxidation in foods and biological systems are reviewed. A new classification, according to the compounds produced in the oxidation phases, is suggested as follows: measurement of oxygen absorption, conjugated dienes, peroxide values, and decomposition of lipid peroxides. Both conjugated dienes (propagation phase) and thiobarbituric acid (termination phase) methods are the most used, despite their interferences; however, chromatographic methods are more precise and selective. Determination of bioavailability and metabolic pathway of the antioxidants in the human body are problems to be solved in the devel opment of new biological antioxidants. [ABSTRACT FROM PUBLISHER]