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Abstract: Bioactive peptides in foods may have health-promoting properties, including effects on cell proliferation. The goal of this study was to determine whether physiological digestion of chickpea protein using pepsin and pancreatin could release this type of peptides. The THP-1 and Caco-2 cell lines were used as in vitro models to determine the effects of the hydrolysates on cell proliferation. Hydrolysates with a high degree of hydrolysis inhibited the growth of Caco-2 cells by up to 45%, suggesting that chickpea-derived peptides might inhibit the growth of tumors in the colon. Proliferation of THP-1 cells was inhibited by up to 78% by direct exposure to the hydrolysates. Nevertheless, bioavailability experiments using differentiated Caco-2 cells as a model of the intestinal barrier, in co-culture with THP-1 cells, showed that proliferation of the monocytic THP-1 cells was actually enhanced by up to 66%, which could represent an immunomodulatory activity. [Copyright &y& Elsevier]

Abstract: The influence of caseinophosphopeptides (CPPs) added to fruit beverage versus milk based fruit beverage upon zinc retention, transport, and uptake, as well as the influence of Fe supplementation, were studied using a combined simulated gastrointestinal digestion/Caco-2 cell system. Zinc retention, transport, and uptake of milk based fruit beverage was 4- to 5-fold greater than that of fruit beverages with or without CPPs – no statistically significant differences being observed in relation to the presence or absence of CPPs. Possibly, a slow release of CPPs throughout the digestive tract, as can be expected to take place during the digestion of casein, has a more beneficial effect than the addition of preformed CPPs upon Zn availability. A significantly negative Fe×Zn interaction in relation to Zn retention and uptake was observed in fruit beverages. This should be taken into account in the formulation of such beverages, where the supplementing of both of these mineral elements is common practice. [Copyright &y& Elsevier]