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[Display omitted] • SMPPs were prepared using different combinations of encapsulated material. • Microencapsulated L. rhamnosus GG had better viability after spray drying (> 10 8 CFU/g). • Carrier agents had a significant effect on bioactive compounds & antioxidant activity. • Encapsulated probiotics had maximum survivability in GIT than control probiotics. This study investigated spray drying a method for microencapsulating Lacticaseibacillus rhamnosus GG using a gastrointestinal resistant composite matrix. An encapsulate composite matrix comprising green banana flour (GBF) blended with maltodextrin (MD) and gum arabic (GA). The morphology of resulted microcapsules revealed a near-spherical shape with slight dents and no surface cracks. Encapsulation efficiency and product yield varied significantly among the spray-dried microencapsulated probiotic powder samples (SMPPs). The formulation with the highest GBF concentration (FIV) exhibited maximum post-drying L. rhamnosus GG viability (12.57 ± 0.03 CFU/g) and best survivability during simulated gastrointestinal digestion (9.37 ± 0.05 CFU/g). Additionally, glass transition temperature (T g) analysis indicated good thermal stability of SMPPs (69.3 – 92.9 ℃), while Fourier Transform infrared (FTIR) spectroscopy confirmed the structural integrity of functional groups within microcapsules. The SMPPs characterization also revealed significant variation in moisture content, water activity, viscosity, and particle size. Moreover, SMPPs exhibited differences in total phenolic and flavonoid, along with antioxidant activity and color values throughout the study. These results suggested that increasing GBF concentration within the encapsulating matrix, while reducing the amount of other composite materials, may offer enhanced protection to L. rhamnosus GG during simulated gastrointestinal conditions, likely due to the gastrointestinal resistance properties of GBF. [ABSTRACT FROM AUTHOR]

[Display omitted] • Particles enriched with cholecalciferol were produced. • Plasmolysis treatment increased impregnation efficiency. • S. pastorianus waste is a low-cost option for micronutrient biosorption. • Spray drying after plasmolysis provided effective vitamin D 3 protection and retention. Vitamin D 3 (cholecalciferol) plays a crucial role in various physiological processes. However, vitamin D 3 deficiency is a major public health problem affecting millions of people. Therefore, it is important to develop effective strategies that ensure the protection and stability of this important vitamin for food supplementation and fortification. This work aimed to impregnate intact and plasmolyzed Saccharomyces pastorianus brewer's yeast biomass with cholecalciferol using a biosorption process followed by spray drying to characterize the obtained material in terms of morphology, average particle size, zeta potential, moisture, water activity, FT-IR, and the stability of the encapsulated vitamin during the drying and storage process. Plasmolysis proved to be an effective method for improving the biosorption efficiency, retention during spray drying, and stability of vitamin D 3. In addition, this process promoted an increase in cell size, which favored the dispersion stability of the system, as evidenced by the zeta potential values. These results contribute to the understanding of a new method for delivering this vitamin that conforms to environmentally conscious practices. [ABSTRACT FROM AUTHOR]

[Display omitted] • Gum Arabic has a higher emulsifying activity than soluble pea protein. • Quercetin addition did not influence the oil encapsulation efficiency and yield. • Electrospray drying promoted the formation of round microcapsules. • Encapsulated oil showed improved stability when quercetin was added in the oil. Complex coacervation can be used for controlled delivery of bioactive compounds (i.e., flaxseed oil and quercetin). This study investigated the co-encapsulation of flaxseed oil and quercetin by complex coacervation using soluble pea protein (SPP) and gum arabic (GA) as shell materials, followed by innovative electrostatic spray drying (ES). The dried system was analyzed through encapsulation efficiency (EE) and yield (EY), morphological and physicochemical properties, and stability for 60 days. Small droplet size emulsions were produced by GA (in the first step of complex coacervation) due to its greater emulsifying activity than SPP. Oil EY and EE, moisture, and water activity in dried compositions ranged from 75.7 to 75.6, 76.0–73.4 %, 3.4–4.1 %, and 0.1–0.2, respectively. Spherical microcapsules were created with small and aggregated particle size but stable for 60 days. An amount of 8 % of quercetin remained in the dried coacervates after 60 days, with low hydroperoxide production. In summary, when GA is used as the emulsifier and SPP as the second biopolymer in the coacervation process, suitable coacervates for food applications are obtained, with ES being a novel alternative to obtain coacervates in powder, with improved stability for encapsulated compounds. As a result, this study helps provide a new delivery system option and sheds light on how the characteristics of biopolymers and the drying process affect coacervate formation. [ABSTRACT FROM AUTHOR]

[Display omitted] • Polysaccharides (5 %) were added to concentrated milk before enzymatic hydrolysis. • Low-lactose milk powder was prepared at an inlet temperature of 185 °C. • Protein-polysaccharide interactions reduced browning in low-lactose milk powder. Lactose hydrolysed concentrated milk was prepared using β-galactosidase enzyme (4.76U/mL) with a reaction period of 12 h at 4 °C. Addition of polysaccharides (5 % maltodextrin/β-cyclodextrin) to concentrated milk either before or after lactose hydrolysis did not result in significant differences (p > 0.05) in degree of hydrolysis (% DH) of lactose and residual lactose content (%). Three different inlet temperatures (165 °C, 175 °C and 185 °C) were used for the preparation of powders which were later characterised based on physico-chemical and maillard browning characteristics. Moisture content, solubility and available lysine content of the powders decreased significantly, whereas, browning parameters i.e., browning index, 5-hydroxymethylfurfural, furosine content increased significantly (p < 0.05) with an increase in inlet air temperature. The powder was finally prepared with 5 % polysaccharide and an inlet air temperature of 185 °C which reduced maillard browning. Protein-polysaccharide interactions were identified using Fourier Transform infrared spectroscopy, fluorescence spectroscopy and determination of free amino groups in the powder samples. Maltodextrin and β-cyclodextrin containing powder samples exhibited lower free amino groups and higher degree of graft value as compared to control sample which indicated protein-polysaccharide interactions. Results obtained from Fourier Transform infrared spectroscopy also confirmed strong protein-polysaccharide interactions, moreover a significant decrease in fluorescence intensity was also observed in the powder samples. These interactions between the proteins and polysaccharides reduced the maillard browning in powders. [ABSTRACT FROM AUTHOR]

[Display omitted] • A single droplet dryer can simulate temperature-time trajectories of a spray dryer. • Temperature-time trajectories influence drying behaviour. • Drying at inlet air temperature after locking increases β-galactosidase inactivation. • A model helps to predict droplet drying behaviour and β-galactosidase inactivation. Optimization procedures for industrial spray drying processes mainly rely on empirical understanding. Mechanistic understanding of the process is limited, but can be enhanced by studying the drying of single droplets. We here report on a new sessile single droplet drying platform, using two air streams to represent the inlet and outlet air of a spray dryer to simulate changing conditions in a spray dryer. Accurate temperature measurements confirmed the temperature profiles and their imposition onto a drying droplet. Single droplets of solutions containing β-galactosidase and maltodextrin were dried with different temperature-time trajectories, with the inactivation of the enzyme as indicator for the thermal load on the droplet. The locking point is found to be an important parameter: the air temperature before this point does not influence the enzyme inactivation much, but a high air temperature after the locking point results in significant inactivation. The β-galactosidase inactivation was also successfully predicted with a coupled drying and inactivation model. [ABSTRACT FROM AUTHOR]

[Display omitted] • The impact of 5 different drying techniques on C. vulgaris was investigated. • Agitated thin film drying (ATFD) improved multiple techno-functional properties. • ATFD increased in vitro digestibility. • Pulse combustion drying was found to increase cacao odor. • ATFD resulted in the creation of an earthy off-flavor. Drying is a necessary step in the microalgae production chain to reduce microbial load and oxidative degradation of the end product. Depending on the differences in applied temperature and treatment time, the process of drying can have a substantial impact on protein quality and aroma, important characteristics determining the incorporation potential in food products. In this study, we compared the drying of heterotrophic Chorella vulgaris with both innovative (agitated thin film drying (ATFD), pulse combustion drying (PCD) and solar drying (SolD)) and commonly used drying techniques (spray drying (SprD) and freeze drying (FD)). To evaluate the impact on protein quality, we evaluated techno-functional properties, in vitro digestibility (INFOGEST) as well as protein denaturation using differential scanning calorimetry (DSC). A sensory analysis was performed by a trained expert panel, combined with headspace solid-phase microextraction (HS-SPME) - gas chromatography-mass spectrometry (GC–MS) to determine volatile organic compounds (VOCs). ATFD was found to increase techno-functional properties such as gelling, water holding and solubility as well as in vitro protein digestibility. These observations could be related to induced cell disruption and protein denaturation by ATFD. Sensory analysis indicated an increased earthy off-flavor after ATFD. Interestingly, the high-temperature PCD led to an increase in cacao odor while low-temperature FD resulted in lower flavor, odors and VOCs. These results demonstrate that protein quality and sensorial properties of C. vulgaris can be steered through the type of drying, which could help in the selection of application-specific drying methods. Overall, this work could promote the incorporation of microalgal single cell proteins in different innovative food products. [ABSTRACT FROM AUTHOR]

[Display omitted] • Phenolic extracts of leaves of forestry biomass were encapsulated. • Encapsulated microparticles have elevated glass transition temperatures. • Microencapsulation helped in masking unwanted flavor. • Stomach phase has higher release of encapsulated phenolic extract. Sugar maple leaves (SML), usually considered residue plant biomass and discarded accordingly, contain a considerable amount of phenolic antioxidants. In this study, SML phenolics were extracted employing both advanced (homogenization pretreated ultrasound-assisted extraction) and conventional (maceration) methods followed by their encapsulation by freeze drying and spray drying using a combination of maltodextrin and gum arabic as coating agents. Detailed physicochemical analyses revealed that the encapsulated microparticles had high solubility (>90 %) and encapsulation efficiency (>95 %), acceptable thermal stability with good handling properties. Phenolic compounds were completely released from microparticles during simulated gastric conditions. The microparticles influenced the bioaccessibility of more than 43 % of the phenolic fraction in the intestinal phase. The antioxidant capacity of the microparticles was preserved during storage. These findings suggest the effectiveness of the microencapsulation process for producing high quality microparticles of SML phenolic extracts and the possibility of their use in the food, nutraceutical, bio-pharmaceutical sectors. [ABSTRACT FROM AUTHOR]

[Display omitted] • Rice bran hydrolyzed protein reduced the surface tension of grape juice. • Enzymatic hydrolysis increased protein content on the microparticle surface. • Rice bran protein hydrolyzate increased anthocyanin retention in microparticles. • Hydrolyzed proteins did not compromise the microencapsulation efficiency. • Protein hydrolysis increased the antioxidant capacity of microparticles. There is a growing demand for the food industry to find appealing matrices that display a clean and sustainable label capable of replacing animal proteins in the encapsulation market for natural pigments. Therefore, this study evaluated the impact of enzymatic hydrolysis by Flavourzyme protease on the encapsulation properties of rice bran proteins, aiming to protect anthocyanins in grape juice microparticles. To achieve this, rice bran protein hydrolysates (RPH) with low (5%, LRPH), medium (10%, MRPH), and high (15%, HRPH) degrees of hydrolysis (DH) were used combined with maltodextrin as carrier agents for the microencapsulation of grape juice by spray drying. The feed solutions contained 1 g of carrier agents (CA)/g of soluble solids from the juice (SS) and protein: a 15% CA ratio. Non-hydrolyzed rice protein was used as a carrier agent to obtain a control sample to evaluate the effect of enzymatic hydrolysis on the microencapsulation of grape juice. Protein modification increased the surface activity of the protein and its ability to migrate to the surface of the microparticles, forming a protective film, as observed by X-ray photoelectron spectroscopy. Using HRPH as a carrier agent combined with maltodextrin improved the internal and total anthocyanin retention, antioxidant capacity measured by DPPH and ABTS+ assays, and powder recovery compared to the control sample, and increased DH reduced particle size and powder stickiness. These particles were more homogeneous, rough, and without cracks. The microencapsulation efficiency was above 70%. All powders exhibited low values of hygroscopicity and degree of caking. Therefore, enzymatic hydrolysis proves to be a promising alternative for improving rice bran protein's encapsulating properties since using RPH as an encapsulating agent conferred greater protection of anthocyanins in microparticles. Moreover, the HRPH sample exhibited the most favorable outcomes overall, indicating its potential for prospective utilization in the market, supported by its elevated Tg. [ABSTRACT FROM AUTHOR]

[Display omitted] • Eight chocolate formulations added with phenolic-rich powders were manufactured. • Evaluated phenolic content and sensory profiles. • Powders induced modifications to color, flavor, and texture. • Wine powder reduced cocoa butter dominance in white chocolates. • The addition of powders intensified bitterness in dark chocolates. Cabernet Sauvignon (CS) and a combination of Cabernet Sauvignon with blueberry extract (CS + B), were spray dried (using maltodextrin DE10, 13.5% w / w as a carrier) to obtain two types of phenolic-rich powders. The addition of blueberry to CS increased phenolic compounds content by 16%. Eight chocolate formulations were obtained by modifying concentrations of cocoa solids, cocoa butter, and sugar. Six of the samples were added with 10% w/w of phenolic-rich powder, while two of them remained as powder-free controls. The anthocyanin and flavan-3-ol profiles of chocolates were determined by HPLC-DAD-MS and HPLC-MS, respectively. In addition, the sensory dynamic profile of samples was assessed by Temporal Dominance of Sensations with a consumer panel. Results showed that the addition of phenolic-rich powders produced a significant increase in the anthocyanin composition obtaining the highest anthocyanin content in the white chocolate added with CS + B powder. On the other hand, adding 10% of CS powder to dark chocolate (55% cocoa pellets) did not result in a significant increase in phenolic compounds. The addition of phenolic-rich powders to chocolates influenced visual color, texture, and taste, leading to new products with distinctive characteristics and increasing the possibility of using phenolic-rich powders as innovative and healthy ingredients. [ABSTRACT FROM AUTHOR]

Schematic of the process, highlighting the major changes observed and the compounds identified post Maillard conjugation in the Amaranth-seaweed blends. [Display omitted] • Formation of amaranth protein-red seaweed conjugates and reaction-products via ultrasonication. • Production of functional blends without formation of advanced glycation end-products. • Retention/enhancement of antioxidant in blends post-ultrasonication and spray drying. Plant-based proteins, represented by amaranth in our study, embrace a potential as an ingredient for the functional-food formulation. However, their efficacy is hindered by inherent limitations in solubility, emulsification, and antioxidant traits. The Maillard reaction, a complex chemical-process resulting in a diverse array of products, including Maillard conjugates and Maillard reaction products (MRPs), can employ variable effects on these specific attributes. To elucidate the influence of this reaction and the MRPs on the aforementioned properties, we used a complex blend of dehydrated seaweed Gracilaria and amaranth protein to create a conjugate-MRP blend. Our investigations revealed that the resultant incorporation enhanced solubility, emulsification, and antioxidant properties, while the intermediates formed did not progress to advanced glycation stages. This change is likely attributed to the dual effect of conjugates that altered the secondary protein structure, while the generation and/or preservation of MRPs post ultrasonication and spray drying enhanced its antioxidant potential. [ABSTRACT FROM AUTHOR]

[Display omitted] • High-quality Chenpi EO was obtained by supercritical fluid extraction at 25 MPa. • Chenpi flavouring essence was optimally prepared by microencapsulation of EO. • Microencapsulation improved thermostability and water dispersibility of Chenpi EO. • Chenpi flavouring essence reduced 20% usage of sucrose and salt via specific aroma. • Chenpi flavouring essence had dual effects on sweetness and saltiness enhancement. To obtain flavouring essence with application potential in sugar and salt-reduced foods, the optimal strategy for extraction and microencapsulation of essential oil (EO) from Chenpi was investigated. UPLC-QTOF-MS/MS and liquid–liquid-extraction-GC–MS confirmed the selectivity for volatiles ranked in hydrodistillation > supercritical fluid extraction > solvent extraction. The aroma characteristic of Chenpi EO was distinguished by 33 key volatiles (screened out via headspace-SPME-GC–MS) and quantitative descriptive analysis. EO extracted by supercritical fluid extraction was preferred for preserving the original aroma of Chenpi and displaying more fruity, honey and floral. Chenpi flavouring essence with superior encapsulation efficiency, particle size, water dispersibility, and thermostability was obtained through optimally microencapsulating EO with gum arabic and maltodextrin (1:1) by high-pressure homogenization coupled with spray drying. Chenpi flavouring essence was able to reduce the usage of sugar and salt by 20 % via enhancing flavour perception of sweetness and saltiness. This study first developed a flavouring essence promisingly effective in both sugar and salt-reduced foods. [ABSTRACT FROM AUTHOR]

Soursop is seasonal and highly perishable fruit, which limits its commercialization. Thus it is necessary to conserve its pulp so that it is available throughout the year. One of the most common forms of fruit preservation is by dehydration. This work had an objective to dehydrate soursop pulp by spray drying at optimum conditions and to analyze the retention of bioactive and volatile compounds in soursop powder, besides analyzing its antioxidant capacity. The total phenolics, carotenoids and flavonoids contents were determined, while volatile compounds were analyzed by Stir Bar Sorptive Extraction (SBSE) coupled with GC–MS system. The total content of the phenolic compounds and flavonoids in the fresh pulp were 160.28 mg of GAE/100 g and 87.17 mg of quercetin/100 g, respectively while for rehydrated dried powder their values were 158.95 mg of GAE/100 g and 85.17 mg of quercetin/100 g, respectively. The total phenolic compounds, flavonoids and antioxidant capacity did not show any significant difference (p <.05) between the fresh fruit pulp and dehydrated powder. A total of 85 volatile compounds were identified, of which 33 were esters, representing the major class of organic compounds, 15 were terpenes, 10 aldehydes, 7 acids, 5 alcohols, 5 lactones, 3 ketones, and 6 other compounds. Of the total 85 compounds, identified in soursop pulp, 58 compounds were identified in the rehydrated dried powder. The principal compounds for both samples were methyl (E)-2-hexenoate, methyl hexanoate and methyl (E)-2-butanoate, which contribute to soursop aroma according to their Odor Active Values (OAV). Considering that there was no significant difference (p <.05) between fresh pulp and the rehydrated dried powder in concentrations of bioactive compounds and even with the reduction in the concentration of the main volatile compounds while the OAVs of these compounds were relatively high, it is concluded that spray dried powder of soursop pulp retains its nutritional and aroma quality, besides maintaining the antioxidant capacity. Unlabelled Image • The drying process showed good retention of bioactive compounds and antioxidant capacity. • A SBSE-GC/MS identified a total of 85 and 58 compounds in soursop pulp and powder respectively. • Thirty one volatiles were selected as key aroma compounds in soursop pulp. • Most of the key aroma compounds of soursop were retained in the dehydrated powder. [ABSTRACT FROM AUTHOR]

[Display omitted] • Ø The emulsifying activity and gel strength of spray-dried egg yolk (SD-EY) were decreased. • SD-EY showed larger aggregate particles, reduced hydrophobicity, and enhanced zeta potential. • Ø N-glycosylation modification in SD-EY altered surface properties, affecting gelation and emulsification. The production of egg yolk powder often involves critical processes such as pasteurization and spray drying, however, these thermal processes will inevitably affect the functional properties of egg yolk (especially gelation and emulsification). The aim of this study was to elucidate the mechanism of the effect of pasteurized egg yolk (P-EY) and spray-dried egg yolk (SD-EY) on the functional properties through quantitative N-glycoproteomic. The results showed, compared with fresh egg yolk (F-EY), emulsifying property of mild heat-treated P-EY was slightly reduced while the gelation property did not undergo significant changes, whereas emulsifying activity (EAI) and gelation strength of vigorously heat-treated SD-EY were significantly reduced by 48.72 % and 35.73 %, respectively. During thermal processing in SD-EY, larger aggregate particles (particle size ∼10 um) were formed, and the surface hydrophobicity was reduced (93.0 %) and the zeta potential was enhanced (62.8 %). The results of quantitative N-glycoproteomic showed that 13 N-glycosylated proteins (APOB, vitellogenin, etc.) were down-regulated while only 2 N-glycosylated proteins were up-regulated; 21 N-glycosylation sites were down-regulated and 2 N-glycosylation sites were up-regulated in SD-EY, suggesting that covalent cross-linking of protein N-glycoproteins may have occurred in the process of spray-drying, which altered molecular physicochemical characteristics of the yolk solution that further affecting the processing properties of egg yolk. [ABSTRACT FROM AUTHOR]

[Display omitted] • Okara is an agro-industrial waste, plenty of nutrients easily consumable by microorganisms. • LCA was used to evaluate the eco-impacts of producing okara fermented powders. • Conditioning, defatting, fermentation, dehydration & storage were the processes' operating conditions. • Conditioning and dehydration-storage were the hotspots having the largest impacts. • LCA enabled to quantify eco-impact for okara fermentation, dehydration and storage. Okara is the insoluble pulp that remains after the grinding and filtration of soybeans during the production of soymilk and tofu. As it retains a noteworthy quantity of nutrients, there has been an increasing emphasis in the utilization of this residue for the development of sustainable processes. This study focused on assessing the environmental impact of employing okara as a medium for fermenting and dehydrating probiotic bacteria at laboratory scale. The evaluation was carried out using the Life Cycle Assessment (LCA) methodology, considering the entire process lifecycle. Whole okara and defatted okara were used as culture media for Lactiplantibacillus plantarum CIDCA 83114, followed by dehydration (either freeze-drying or spray-drying) and subsequent storage. For the purpose of comparison, both scenarios (whole and defatted okara) were evaluated using 1 kg of dehydrated final product for storage, as functional unit. Based on experimental results, the conservation of the received okara and the dehydration-storage (e.g., freezing and freeze-drying) phases were identified as the most significant environmental hotspots responsible for the most substantial impacts of the processes. The use of LCA facilitated the measurement of the environmental effects linked to the reutilization of okara as an agro-industrial residue, thus providing quantitative support when engineering its sustainable valorization. [ABSTRACT FROM AUTHOR]

[Display omitted] • Biscuit dough was prepared by adding free and encapsulated cells separately. • Probiotic biscuit was prepared at different baking temperatures and baking times. • The viability of free and encapsulated cells during baking was studied. • Physicochemical properties of probiotic biscuits were determined. • Log-linear model was the best in predicting probiotic cells' inactivation kinetics. The rising popularity of probiotic food in the diet for improved health benefits leads to the development of new probiotic functional foods. In general, biscuit is a long-shelf-life snack product that can be consumed straight from the pack without further processing. Although the development of probiotic bakery products is an innovative approach to market expansion, the infusion of probiotics in biscuits to produce probiotic biscuits has not been explored because of the complexity of the baking process. Therefore, this study aimed to evaluate the impact of baking conditions (160, 180, 200, and 220 °C) on the viability of free and encapsulated probiotic Lactobacillus acidophilus NCDC 016 cells by adding them into biscuit dough separately and baking for up to 600 sec. The cells were encapsulated using 20 % maltodextrin and 8.51 % gum arabic as a wall material and spray drying at an inlet and outlet air temperature of 150 and 55 ± 2 °C, respectively. At different baking temperatures (160, 180, 200, and 220 °C), the viability of probiotic (free and encapsulated) cells, the physicochemical properties of biscuits, and the inactivation kinetics of cells were examined by withdrawing samples every 120 sec. The survivability of encapsulated cells was observed to be higher than free cells at 160 and 180 °C for 600 sec. The moisture content and water activity were found to be higher and lower, respectively for encapsulated probiotic biscuits than for the biscuit containing free cells. The observed results of higher cell viability at 200 °C, 360 sec (5.38 log CFU/g) than at 180 °C, 600 sec (5.02 log CFU/g) can be explained by the time–temperature combination. Thus, producing the probiotic biscuit at baking conditions of 200 °C and 360 min is possible, providing the cell viability of 5 log CFU/g of probiotic biscuit. Further, the inactivation kinetics of cells were predicted by log-linear, Weibull, log-logistic, Gompertz, and Buchanan models. Under all baking conditions, the log-linear model was the best model for describing the data of encapsulated and free cells. [ABSTRACT FROM AUTHOR]

[Display omitted] • Recrystallized spray-dried corn starch (RSDCS) was prepared. • Effect of spray drying conditions on structure of the RSDCSs was investigated. • Correlations between structure and digestibility of the RSDCSs were analyzed. • Spray drying is an effective way to produce starch products with lower digestibility. Spray drying has been widely applied in food industry due to its efficiency and low cost. Exploring feasibility to prepare resistant starch (RS) via spray drying could open up new route to produce starch-based products with low glycemic index efficiently. In this study, effects of spray drying operating conditions on the structure and digestibility of recrystallized spray-dried corn starch (RSDCS) were explored. Apparent amylose content (AAC) and swelling power (SP) of the RSDCSs increased after the spray drying and recrystallization. Particle size of the RSDCSs decreased significantly with increase of compressed air flow and decrease of starch suspension concentration. Furthermore, the short-range order, long-range order, and content of RS in the RSDCSs decreased with increase of compressed air flow and starch suspension concentration. The Pearson's correlation analysis showed that digestive properties of the RSDCSs were mainly related to the short-range ordered structure and crystalline structure. Moreover, Mantel analysis revealed that operating conditions changed the digestibility of the RSDCSs through impacting crystalline structure, AAC and SP. The highest content of RS in the RSDCSs (23.08%) was increased near 2.6 times comparing to that of native corn starch (9.02%). [ABSTRACT FROM AUTHOR]

[Display omitted] • We reported the L. reuteri microencapsulation by spray drying, and its potential as component of an infant formula. • Use of infant formula for microencapsulation resulted in high encapsulation efficiency and storage stability. • The microencapsulated L.reuteri in IF maintained high viability after dilution at 70 °C. • The use of IF as wall material as an alternative to protect the probiotic L. reuteri for application in infant formulas. This study aimed to evaluate the survival capacity of the probiotic culture Limosilactobacillus reuteri (DSM 23878) to microencapsulation by spray drying, and its potential as component of an infant formula. Preliminary tests were performed between skim milk (SM) and infant formula (IF) as wall material and two inlet temperatures, evaluating the encapsulation efficiency, moisture content, water activity and stability, to choose the drying parameters. After drying in optimized conditions, the powder of microencapsulated L. reuteri was characterized and the viability after dilution in an infant formula at 70 °C was determined. In addition, the survival rate throughout 360 days of storage was assessed. As results, encapsulation efficiency was superior to 90 % in both wall materials. However, the use of IF as for microencapsulation produced microparticles with lower water activity (Aw) and moisture, as compared with the SM. Final microparticles produced with IF as wall material presented values of Aw, moisture content, and particle diameter averaged 0.11 ± 0.02, 2.10 ± 0.35 % and 10.30 ± 0.12 μm, respectively. The viability of microencapsulated L.reuteri decreased 1 Log CFU/mL after dilution at 70 °C and the powder maintained a survivor of 73.5 % after 365 days of storage at 4 °C. Thus, the microencapsulation by spray drying under the conditions of this study proved to be an effective technique to protect the probiotic L. reuteri for application in infant formulas, obtaining an adequate number of viable cells after reconstitution at 70 °C and during long time the storage. [ABSTRACT FROM AUTHOR]

[Display omitted] • Particles of brewer's spent yeasts (BSY) loaded with propolis were produced. • Red propolis' phenolic diversity remained after particle production. • Particle production affected the phenolics of propolis after simulated digestion. • Particle production decreased the antioxidant activity after epithelial transport. • Particle production preserved the anti-inflammatory activity after epithelial transport. Red propolis from northeast Brazil contains mainly isoflavonoids as bioactive compounds, and its consumption may counteract unregulated and exacerbated formation of reactive oxygen species and inflammatory cytokines/chemokines. Moreover, the production of particles using sustainable carriers have been studied to increase the use of propolis as a functional food ingredient. Hence, the objective of this work was to investigate the effects of simulated gastrointestinal digestion followed by a cell-based epithelial transport on phenolic profile, anti-inflammatory and antioxidant activities of particles of brewer's spent yeasts (BSY) loaded with ethanolic extract of Brazilian red propolis (EEP). As a result, the EEP phenolic diversity decreased throughout the simulated gastrointestinal system, and was modulated by the particle production, as detected by high-performance liquid chromatography – electrospray ionization – quadrupole-time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS). Concomitantly, the antioxidant activity, as assessed by the ability to scavenge peroxyl and superoxide radicals, hydrogen peroxide, and hypochlorous acid, generally decreased at a higher extent for the particles of EEP with BSY (EEP-BSY) throughout the experiments. Nonetheless, after epithelial transport through the Caco-2 cell monolayer, the basolateral fraction of both EEP-BSY and EEP decreased the activation of pro-inflammatory transcription factor NF-κB by 83% and 65%, respectively, as well as the release of TNF-α (up to 51% and 38%, respectively), and CXCL2/MIP-2 (up to 33% and 25%, respectively). Therefore, BSY may be an interesting carrier for EEP bioencapsulation, since it preserves its anti-inflammatory activity. Further studies should be encouraged to investigate the feasibility of adding it in formulations of functional foods, considering its effect on sensory attributes. [ABSTRACT FROM AUTHOR]

[Display omitted] • Powder milk presents three different alternatives of production. • The main powder milk process recommends the use of a multi-effect evaporator. • Thermal/mechanical vapor recompression improves the evaporator's energy efficiency. • Cheese and yogurt powder each feature a unique type of processing. • Physical properties are crucial for managing dairy powder products. Knowledge of powdered dairy products was addressed, emphasizing powdered milk, cheese, and yogurt. These are very versatile products, and because they are within the field of innovations in developing dairy products, their production and application have been encouraged. This work aimed to reveal the possibilities of obtaining an approach to powder dairy products, the environmental and economic aspects involved, the physical properties analysis, and finally a conclusions including the findings and prospects. When comparing possible processes of powder milk obtaining the preferred comprises the following steps: fat standardization; pasteurization; evaporation using a multi-effect evaporator with the inclusion of one of two options to increase the evaporator's energy efficiency: thermal or mechanical vapor recompression; dehydration (spray drying); and adding an emulsifier (lecithin) or using the fluidized bed dryer or return of fines, resulting in a powder product with instantaneous dissolution. The following sequence is recommended for cheese powder: ingredients addition, melting, dehydration (spray drying), and finalizing with cooling. Yogurt powder has its production process initiated by agitation, ultrafiltration, and spray drying. Concerning the physical properties, they are crucial for managing dairy powder products during the final drying process and for their use as food ingredients. [ABSTRACT FROM AUTHOR]

[Display omitted] Rice bran protein concentrate (RPC), an industrial by-product, may emerge as a green alternative for substituting animal proteins in microencapsulating compounds of interest. This study applied RPC, combined with maltodextrin (MD) as carrier agents, in the spray drying of grape juice, a product rich in these bioactive compounds, seeking to protect anthocyanins from degradation. The effects of carrier agent concentration [C: 0.75, 1.00, and 1.25 g of carrier agents (CA)/g of soluble solids of the juice (SS)] and RPC:CA ratio (P: 0%, as a control sample, 5%, 10%, 15%, and 20%) on anthocyanin retention and powder properties were evaluated. At 1.00 g CA/g SS, the internal and total retentions of anthocyanins improved by 2.4 and 3.2 times, respectively, when the RPC:CA ratio increased from 0% to 20%. The protein also exhibited excellent surface activity on the grape juice and positively influenced the physicochemical properties of the microparticles. There was a reduction in stickiness, degree of caking, and hygroscopicity, in addition to an increased antioxidant capacity when protein was used in combination with MD, especially at 1.00 and 1.25 g CA/g SS. Therefore, this study demonstrated that RPC could enhance the protection of anthocyanins during the spray drying of grape juice. [ABSTRACT FROM AUTHOR]

[Display omitted] • Coffees inoculated with dried yeast had higher concentrations of volatile compounds. • The dried yeasts were metabolically active. • The most suitable yeast for the natural process would be dried S. cerevisiae. • The most suitable yeast for the pulped process would be dried T. delbrueckii. Using starter culture in liquid form is not economically viable in the coffee fermentation process. This work aimed to compare the fermentative performances of fresh and microencapsulated yeasts in coffee under self-induced anaerobic fermentation (SIAF). The inoculum permanence was monitored, and sugars, alcohols, acids, and volatile compounds were analyzed by chromatography. In addition, sensory analysis was performed on roasted beans. After 180 h of fermentation in the natural process, microencapsulated Torulaspora delbrueckii (MT) (7.97 × 107 cells/g) showed a higher population than fresh Torulaspora delbrueckii (FT) (1.76 × 107 cells/g). The same acids and volatile compounds were detected in coffees with fresh and microencapsulated yeast. However, the yeast state influenced the concentration of the compounds. In pulped coffee, the coffee inoculated with microencapsulated Saccharomyces cerevisiae (MS) obtained the highest concentration of alcohols, esters, pyrazines, and others compared with fresh Saccharomyces cerevisiae (FS), with an increase of up to 47%. Furthermore, the coffee inoculated with MT obtained the highest concentration in almost all chemical classes in both processes compared with FT. These differences ranged up to 55%. Regarding sensory analysis, coffees inoculated with MS showed dominant notes of fruity, caramel, and nuts in the natural process. Otherwise, in pulped process, coffees inoculated with MT showed caramel, honey, and nuts. Therefore, the microencapsulated yeasts were metabolically active and may be considered with commercial potential. Considering the parameters analyzed, the most suitable yeast for natural and pulped processing would be MS and MT, respectively. [ABSTRACT FROM AUTHOR]

[Display omitted] • Tucumã oil microparticles are yellow, have small diameters and high β-carotene content. • The microparticles presented high carotenoid and oxidative stability after storage. • The microparticles showed low β-carotene bioaccessibility after simulated digestion. • Spray chilling could improve β-carotene bioaccessibility compared to the pure oil. The aim of this work was to produce tucumã oil (PO) microparticles using different encapsulation methods, and to evaluate their properties, storage stability and bioaccessibility of the encapsulated β-carotene. Gum Arabic was used as carrier for spray drying (SD), while vegetable fat was the wall material for spray chilling (SC) and the combination of the methods (SDC). Powders were yellow (hue angle around 80°) and presented particles with small mean diameters (1.57–2.30 µm). PO and the microparticles possess high β-carotene contents (∼0.35–22 mg/g). However, some carotenoid loss was observed in the particles after encapsulation by SD and SDC (around 20%). After 90 days of storage, SDC particles presented the lowest degradation of total carotenoids (∼5%), while SD samples showed the highest loss (∼21%). Yet, the latter had the lowest contents of conjugated dienes (4.1–5.3 µmol/g) among treatments. At the end of simulated digestion, PO and the microparticles provided low β-carotene bioaccessibility (<10%), and only SC increased this parameter compared to the pure oil. In conclusion, carotenoid-rich microparticles with attractive color were obtained through microencapsulation of PO by SD, SC and SDC, revealing their potential as natural additives for the development of food products with improved nutritional properties. The SC method stood out for providing microparticles with high carotenoid content and retention, high oxidative stability, and improved β-carotene bioaccessibility. [ABSTRACT FROM AUTHOR]

[Display omitted] • Enteric-soluble materials (ESM) and Jiuzao glutelin-pullulan conjugate (PJC-2) showed ideal protective effect on rhein (RH). • The carrier of ESM and PJC-2 avoided the defects of single-deck material. • ESM-PJC-2 double-deck materials increased RH microcapsules stability. • The micro capsuled-RH antioxidant ability was protected against in vitro digestion. The poor water solubility and rhein (RH) stability limit its application in the functional food industry. In the present study, the RH-loaded water-in-oil-in-water nano emulsion and microcapsules were prepared using the conjugates of pullulan-Jiuzao glutelin (JG) (m/m, 2:1, PJC-2) obtained by Maillard reaction and enteric-soluble materials (polymethlacrylic acid, hydroxypropyl methylcellulose phthalate, cellulose acetate phthalate, and D-mannitol). The effects of different formulations on the microstructure, physicochemical properties, and storage stability of microcapsules were analyzed. The results showed that microcapsules exhibited stability against different external environments. The encapsulation efficiency of RH in the four enteric-soluble-PJC-2 double-deck microcapsules (70.03 ± 3.24%-91.08 ± 4.78%) was significantly improved than PJC-2 ones (61.84 ± 0.47%). The antioxidant activity and stability of RH in the microcapsules were improved (ABTS, 49.7%-113.93%; DPPH, 40.85%-101.82%; FRA, 62.32%-126.42%; and FCA, 70.58%-147.20%) after in vitro simulated digestion and extreme environmental conditions compared to free RH. This work provides a microcapsule based on PJC-2 with enteric-soluble materials for insoluble functional ingredients to improve solubility, stability, and bioactivity in the food industry. [ABSTRACT FROM AUTHOR]

Guaraná is a native fruit of the Amazon rainforest, which presents high levels of phenolic compounds. However, these bioactive compounds may be unstable in food processing and gastrointestinal conditions. Thus, this work aimed to characterize guaraná seed extract (GSE) followed by microencapsulation using a spray-chilling method and with vegetable fat as carrier, as well as to evaluate the particles. Phenolic-rich GSE was produced using 50% (w /w) hydroalcoholic solution and dehydrated by spray drying and lyophilization. Powdered GSE was characterized in relation to its inhibitory activity on digestive enzymes. Solid lipid microparticles (SLM) were evaluated for the retention of bioactive compounds and the release profile of phenolic compounds in simulated gastrointestinal conditions. Powdered GSE showed anti-obesity potential due to the high inhibitory activity of lipase. Regarding the retention of phenolic compounds, at least 75% were detected after 90 days at 25 °C in SLM. Moreover, SLM loaded with 7.5% GSE released approximately 99% of phenolic compounds in simulated gastrointestinal conditions. These results show the efficiency of spray chilling for protection and release of phenolic compounds from GSE, allowing future application in food. Unlabelled Image • Encapsulation promoted the controlled release of phenolic compounds on SGC. • Retention of phenolic compounds into SLM were above 75% after 90 days at 25 °C. • All formulations presented attractive color and free-flowability. [ABSTRACT FROM AUTHOR]

Encapsulation is commonly used to protect flavor compounds against adverse environmental and processing conditions or to provide controlled release in processed foods. Flavor compounds are released during eating and the release rate depends on food breakdown dynamics in the mouth. Two sequential studies were designed to explore the flavor perception of the same flavor in different encapsulation systems. The studies were focused on the interactions between encapsulation technology, particle size and breakdown processes in the mouth. A peppermint flavor was used as a model flavor and encapsulated with different technologies (spray drying, melt extrusion and fluidized bed drying). The encapsulated flavors and a selected combination were incorporated into a soft chewable candy, keeping the total flavor concentration the same for each sample. The chewable candy samples were presented to naïve panelists (n > 30) for the following two evaluations; (1) comparison of overall flavor perception with a 2-alternative forced choice test; and (2) dynamic evaluation of perceived flavor intensity over time during eating and after swallowing using time intensity. The results showed that the overall and dynamic flavor perceptions are greatly affected by the encapsulation technologies and particle sizes, and can be modulated by combining flavor particles produced by different encapsulation technologies depending on the application and desired flavor profile. The results also showed a large perceived flavor intensity variation between panelists, resembling variation among consumers. In an effort to better understand the relationship between the oral processing patterns and flavor perception, we used the JBMB® typing tool which gives four "Mouth Behavior" groups ("Chewers", "Crunchers", "Smooshers" and "Suckers") and explored to determine whether they would account for the variation. Compared with "Chewers" and "Crunchers", "Smooshers" tended to have a slower increase of flavor intensity during eating and a more gradual drop after swallowing. However, this needs to be confirmed with larger numbers of consumers (including suckers who were excluded in this study because they were not sufficient in numbers) and samples with a longer chew time. Unlabelled Image • Encapsulation method affects the flavor perception of encapsulated flavor. • Flavor encapsulated in smaller granules gives stronger flavor perception. • Desired flavor profile can be created by combining different encapsulated flavors. • Differences in consumer perception were tested using a concept of "Mouth Behavior". [ABSTRACT FROM AUTHOR]

Soybean Protein Isolate (SPI) is a by-product from soybean oil industries with good nutritional and functional properties. Denaturation of hydrated SPI can change its properties, allowing the formation of gel-like particles, which can be used to stabilize emulsions without addition of surfactants. SPI microgel particles were produced by denaturation of hydrated SPI, followed by high pressure homogenization or sonication, with different NaCl or NaF contents, aiming the formation of small particles capable to stabilize O/W emulsions and acting as wall material for microencapsulation of soybean oil by spray drying. The presence of NaF in the suspensions decreased the charge intensity of SPI microgels, leading to formation of significantly bigger SPI microgel particles. Based on Creaming Index (CI), all the emulsions were stable for, at least, 21 days at room temperature. The presence of salt affected minimally the droplet size of the emulsions, though there has been an increase in flocculation. All the emulsions presented shear-thinning behavior and a strong shear rate dependence when salt was present in the system. The microspheres produced by spray drying of the emulsions were spherical and showed few aggregate formation. In addition, they presented high values of oil retention (> 80 wt%) and acceptable values of moisture content (< 4 wt%). SPI microgel particles produced by high-pressure homogenization or sonication may be used to stabilize emulsions with low oil contents. These emulsions can be further spray dried to microencapsulate lipophilic compounds using SPI microgels as wall material. Unlabelled Image • Soybean protein microparticles were produced at different ionic strength. • O/W emulsions were produced under sonication and high pressure homogenization. • Emulsions presented good stability against creaming. • Microspheres were produced from Pickering emulsions by spray drying. • Soybean protein microparticles showed ability to encapsulate lipid compounds. [ABSTRACT FROM AUTHOR]

Linseed oil was nanoencapsulated with chia seed mucilage (CSM) as structuring material. Linseed oil nanoparticles (LO-NP) were evaluated regarding particle size distribution, zeta potential, pH, viscosity, encapsulation efficiency, loading capacity, morphology, FT-IR and thermal properties. Furthermore, the nanoparticles were spray-dried, and oxidative stability was evaluated during 28 days under storage at accelerated conditions (40 °C). The bioaccessibility of spray dried nanoparticles (SP LO-NP) was also evaluated after in vitro digestion. Thereafter, SP LO-NP were utilized in the enrichment of orange juice, and physicochemical and sensory evaluation of pure orange juice and orange juice with SP LO-NP were evaluated. Nanoparticles in suspension presented a mean diameter of 356 ± 2.83 nm, zeta potential of –22.75 ± 3.89 mV and encapsulation efficiency of 52%. No significant differences regarding consumer acceptance were observed between pure orange juice and orange juice with SP LO-NP. The results suggest that CSM can be used as structuring material to nanoencapsulate hydrophobic compounds, allowing its solubility in foods with high water content. Furthermore, the SP LO-NP provided a good bioaccessibility to linseed oil after in vitro digestion, which represents an advantage to incorporate the nanoparticles in food. Unlabelled Image • LO-NP were produced with chia seed mucilage (CSM) as structuring material. • SP LO-NP were utilized to orange juice enrichment. • Nanoparticles were successful in protecting linseed oil (LO) against oxidation. • The orange juice with SP LO-NP had a greater sensorial acceptance. [ABSTRACT FROM AUTHOR]

Abstract Spray-dried flaxseed oil microcapsules were produced by designing O/W double-layer emulsions using a whey protein concentrate (WPC) and sodium alginate (SA). The influence of homogenization pressure (5–15 MPa), pH (4–7) and maltodextrin concentration (0.8–7 wt%) on stability of primary and secondary emulsions was investigated, through droplet size and zeta potential measurements. Powders obtained after spray drying were characterized through scanning electron microscopy, encapsulation efficiency and water activity determinations. Flaxseed oil oxidative stability was assessed by measuring peroxide values (PV) and thiobarbituric reactive substances (TBARS) at different microencapsulation processing steps and during powders storage. Droplet sizes of primary emulsions were reduced when increasing homogenization pressures (up to 10 MPa). Zeta potential measurements evidenced double WPC-SA layer formation around oil droplets at pH 5. Encapsulation efficiencies up to 84% were obtained in powdered microcapsules with the highest MD content. Microencapsulation process produced a gradual increment on PV, whereas TBARs slightly increased. Nevertheless, these values were maintained relatively constant after powders storage at −18 and 4 °C for 6 months, and at 20 °C up to 6 weeks and PV did not exceed the maximum allowed for cold pressed oils. Graphical abstract Unlabelled Image Highlights • Emulsion layer-by-layer deposition technology was used to encapsulate flaxseed oil. • Effect of low homogenization pressure and maltodextrin (MD) content was studied. • Microencapsulation process gradually increased peroxide value (PV) of the oil. • Powders did not exceed maximum PV allowed for cold pressed oils during storage. [ABSTRACT FROM AUTHOR]

Abstract This study investigated spray drying of food-grade solid lipid particles (SLN) and nanostructured lipid carriers (NLC) containing ω-3 fish oil. Stable SLN and NLC dispersions with tristearin as carrier lipid were formed by using a combination of Quillaja saponins and high-melting lecithin as emulsifiers. Our specific goal was to study the influence of four different spray drying inlet and outlet temperatures (T inlet/outlet = 140–170 °C/65–95 °C) and two different maltodextrin types (DE 6 and DE 21) with different molecular weights as protective wall materials on the physical and polymorphic stability of the solid lipid particles. The results revealed that the low molecular weight maltodextrin DE 21 was a superior wall material in stabilizing the solid lipid particles. Moreover, the lipid particles spray dried at T inlet/outlet of 140/65 °C exhibited the highest physical and polymorphic stability, whereas using higher T inlet/outlet led to bigger particles which were more prone to polymorphic transition. This was also verified in a 71-day storage test. The findings were explained that by preventing the melting of the tristearin carrier lipid during spray drying, the crystallized lipid particles remained intact inside the amorphous maltodextrin layer and exhibited high physical and polymorphic stability. These findings are important for generating stable food-grade spray dried powders. Graphical abstract Unlabelled Image Highlights • Spray drying of food-grade solid lipid particles was investigated. • Impact of inlet temperature and wall material type upon spray drying was studied. • Low molecular weight maltodextrin was better in stabilizing the solid lipid particles. • The highest physical and polymorphic stability was achieved at T inlet of 140 °C. [ABSTRACT FROM AUTHOR]

Graphical abstract Unlabelled Image Highlights • Chemical and physical properties of native starch achira were modified. • Modified achira starch had good characteristics as an encapsulating material. • For the Hibiscus sabdariffa extract, an encapsulation efficiency of 70% was obtained by spray drying. • Microcapsules of approximately 10 μm were obtained by spray drying. • The microcapsules had excellent antimicrobial activity. [ABSTRACT FROM AUTHOR]

Abstract In this work, the aqueous extract obtained from Brazilian ginseng (Pfaffia glomerata) roots (BGR), rich in beta-ecdysone and fructooligosaccharides (FOS), was powdered by spray drying and freeze drying techniques aiming to obtain a novel functional food product. The effects of these drying techniques on the chemical and nutritional quality, morphological and redispersion properties of the BGR powders were evaluated. The BGR powders obtained by both spray drying and freeze drying techniques maintained their beta-ecdysone and FOS contents after drying, demonstrating the stability of these functional compounds. It was found that the wettability of the powders obtained by different treatments was affected by the drying technique because freeze-dried particles reached the lower values (66 ± 5 s) while spray-dried particles showed a greater time for dispersion into water (150 ± 25 s). This behavior was mainly associated with differences between powder morphological properties since the freeze-dried particles presented a more porous structure, resulting in a greater water diffusivity into microstructure during the redispersion process. Drying process did not affect the storage stability of powders because the glass transition temperature (Tg) for both samples was approximately 160 °C at a relative humidity of 56%. Thus, both BGR powders presented adequate redispersion properties to constitute a new functional tea or even to be used as a functional ingredient in food products. Graphical abstract Unlabelled Image Highlights • An instant powder rich in bioactive compounds was obtained in this work. • Brazilian ginseng extract was dried by spray drying and freeze drying. • The dried powders maintained their fructooligosaccharides and beta-ecdysone contents. • The powders presented satisfactory redispersion properties. [ABSTRACT FROM AUTHOR]

Abstract The stabilizing capacity of crystalline inulin during spray-drying and storage of Lactobacillus plantarum CIDCA 83114 was assessed. In a first step, the physical properties of the matrices were investigated, using amorphous inulin as control. Melting and glass transition temperatures, water sorption isotherms, water activity, and infrared spectra were determined. Microorganisms were spray-dried at a pilot scale in both amorphous and crystalline matrices. After that, scanning electronic and confocal microsopies provided a full landscape about the interactions between microorganisms and crystals, and also the bacterial location within the amorphous matrices. The technological properties of the dehydrated microorganisms (culturability and acidification capacity) during storage at different water activities were also evaluated. Both amorphous and crystalline inulins were adequate matrices to stabilize microorganisms. However, crystalline inulin was more stable than amorphous one, especially when the storage temperature was close to the glass transition temperature, resulting in a better matrix to protect microorganisms during pilot spray-drying and storage. Furthermore, no accumulation of insoluble inulin was observed after resuspending the dehydrated microorganisms in crystalline inulin matrices, which appears as a clear technological advantage with regard to the amorphous one. Considering the prebiotic character of inulin and the probiotic properties of L. plantarum CIDCA 83114, this work developed an integrated approach, both from a fundamental and from an applied viewpoint, supporting the incorporation of such ingredients in the formulation of food products. Graphical abstract Unlabelled Image Highlights • Crystalline and amorphous inulins were used to stabilize L. plantarum CIDCA 83114 during spray-drying at a pilot scale. • Tm and Tg, water sorption isotherms, a w and FTIR were determined to explain the protecting effect. • Both amorphous and crystalline inulin matrices were adequate to stabilize micro-organisms. • Crystalline inulin was more stable than amorphous one, especially when T was close to Tg. • Such behavior explained the better stabilizing capacity of crystalline inulin during storage. [ABSTRACT FROM AUTHOR]

Spray drying was applied for the production of kefir powder. The survival of microorganisms after drying, storage and simulated gastrointestinal (GI) conditions was investigated. Kefir was obtained by fermentation of milk and whey permeate, and was dehydrated directly (traditional kefir) or using different carriers (skim milk, whey permeate and maltodextrin). Low survival (5.5 log and <2 log CFU/g for lactic acid bacteria and yeast respectively) of microorganisms was achieved when kefir was dehydrated without thermoprotectants (carriers). In contrast, survival of the microorganisms was significantly improved in the presence of different carriers. When skim milk (SM) was used as the carrier medium, lactic acid bacteria (LAB) survival was above 9 log CFU/g. In contrast, viability of yeast was dramatically reduced after spray drying in these conditions. When whey permeate was used as the carrier medium, LAB survival was 8 log CFU/g and yeast survival was above 4 log CFU/g. LAB in the kefir powder survived better the simulated GI conditions when spray drying was conducted in SM. LAB in kefir powder sample dehydrated in SM and SM plus maltodextrin remained stable for at least 60 days at 4 °C. Our results demonstrated that spray drying of kefir is a suitable approach to obtain a concentrated kefir-derived product. [ABSTRACT FROM AUTHOR]

Dehydration and thermal stresses are generally considered as two mains factors deactivating probiotic cells during droplet drying, as typically in industrial spray drying for producing active dry probiotics. However, little is known about how cells respond to these interplaying stresses in the short period of drying. This study showed that dehydration process could alleviate the detrimental effect of thermal stress to a certain extent, evidenced by that probiotic cells could withstand higher temperature in a single droplet drying (SDD) process compared to sole heat treatment. During SDD at 90 °C, droplet temperature increased with time, and the inactivation of Lactobacillus rhamnosus GG (LGG) was initially observed at droplet temperature of 61–65 °C. By contrast, the transition from the maintenance of LGG viability to rapid deactivation occurred at around 54 °C in heat treatment without dynamic dehydration. Possible mechanisms for the enhanced thermotolerance were investigated from drying kinetics level and cellular level. The favorable temperature profile and the decrease in droplet water activity during drying may benefit cell survival. The cytoplasmic membrane of LGG was more stable at elevated temperatures of 60–65 °C during drying, which might be related to the high viscosity of semi-dried particles mitigating the leakage of intracellular substances. Trehalose demonstrated a strong thermoprotective effect over lactose in heat treatment, but the protection was less effective at the later stage of drying. These results dissected the influence of the interplaying stresses on probiotic cells for the first time during droplet drying and also suggested possible approaches for improving cell survival in dried particles. Components capable of protecting cellular membrane are recommended for developing protectant formulation in spray drying of probiotics. [ABSTRACT FROM AUTHOR]

[Display omitted] • FD dioscorin had a higher ESI and SD dioscorin had a higher EAI. • GABA induced the exposure of the hydrophobic groups of dioscorin. • GABA changed the conformation of dioscorin by increasing β-sheet and coil contents. • GABA promoted more adsorption of dioscorin to the O/W interface. • MD simulation showed GABA interacts with dioscorin mainly through Coulomb interactions. Many studies have shown that γ-aminobutyric acid (GABA) exhibits various beneficial biological activities, including gut-modulating, neuro-stimulating, and cardio-protecting activities. Naturally, GABA exists in small amounts in yam, which is primarily synthesized by the decarboxylation of L-glutamic acid in the presence of glutamate decarboxylase. Dioscorin, the major tuber storage protein of yam, has been shown to have good solubility and emulsifying activity. However, how GABA interacts with dioscorin and affects their properties has yet to be clarified. In this research, the physicochemical and emulsifying properties of GABA-fortified dioscorin, which was dried by spray drying and freeze drying, were studied. As results, the freeze-dried (FD) dioscorin produced more stable emulsions, while the spray-dried (SD) dioscorin adsorbed more rapidly to oil/water (O/W) interface. The fluorescence spectroscopy, ultraviolet spectroscopy and circular dichroism spectroscopy showed that GABA changed the structure of dioscorin, by exposing its hydrophobic groups. The addition of GABA significantly promoted the adsorption of dioscorin to the O/W interface and prevented droplets coalescence. The results of molecular dynamics simulation (MD) showed that GABA destroyed the H-bond network between dioscorin and water, increased surface hydrophobicity and finally improved the emulsifying properties of dioscorin. [ABSTRACT FROM AUTHOR]

[Display omitted] • Encapsulated Sacha Inchi oil is useful to protect nutraceutical components. • Homogenization technologies and temperature affected microcapsules properties. • In vitro study of spray dried microcapsules showed high Omega-3 bioavailability. Sacha Inchi seed oil (SIO) is rich in omega 3, 6, and 9 fatty acids with important health benefits, but is temperature sensitive. Spray drying is a technology that improves the long-term stability of bioactive compounds. This work aimed to study the effect of three different homogenization techniques on some physical properties and bioavailability of microcapsules of Sacha Inchi seed oil (SIO) emulsions obtained by spray drying. Emulsions were formulated with SIO (5%, w/w), maltodextrin:sodium caseinate as wall material (10%, w/w; 85:15), Tween 20 (1%, w/w) and Span 80 (0.5%, w/w) as surfactants and water up to 100% (w/w). Emulsions were prepared using high-speed (Dispermat D-51580, 18,000 rpm, 10 min), conventional (Mixer K-MLIM50N01, Turbo speed, 5 min), and ultrasound probe (Sonics Materials VCX 750, 35% amplitude, 750 W, 30 min) homogenization. SIO microcapsules were obtained in a Mini Spray B-290 (Büchi) using two inlet temperatures of the drying air (150 and 170 °C). Moisture, density, dissolution rate, hygroscopicity, drying efficiency (EY), encapsulation efficiency (EE), loading capacity, and oil release in digestive fluids in vitro were studied. Results showed that the microcapsules obtained by spray-drying had low moisture values and high encapsulation yield and efficiency values (greater than 50% and 70%, respectively). The thermogravimetric analysis indicates that heat protection was assured, enhancing the shelf life and the ability to withstand thermal food processing. Results suggest that spray-drying encapsulation could be a suitable technology to successfully microencapsulate SIO and enhance the absorption of bioactive compounds in the intestine. This work highlights the use of Latin American biodiversity and spray drying technology to ensure the encapsulation of bioactive compounds. This technology represents an opportunity for the development of new functional foods, improving the safety and quality of conventional foods. [ABSTRACT FROM AUTHOR]

[Display omitted] • 1061 lipids in raw, pasteurized and spray dried goat milk were found by lipidomics. • 185 Different Lipids (DLs) were selected from pairwise comparisons. • Oleic acid (C18:1) were significantly different (P < 0.05). • Five lipids were identified as potential biomarkers. • Glycerophospholipids were more affected by spray drying than pasteurization. The purpose of this study was to evaluate the effects of pasteurization and spray drying on goat milk lipids by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and multiple variable statistics. A total of 1061 lipids assigned to 29 subclasses in raw and thermal-treated groups were identified. One hundred and 85 different lipids (DLs) (VIP ≥ 1 and |Log 2 FC| ≥ 1.0) were selected from pairwise comparisons of goat milk by different treatments. Glycerophospholipids were the most affected subclasses by thermal processes, especially by spray drying. Five potential lipid markers [(DG (16:1_18:0), TG (18:1_22:1_18:2), Cer (t17:2/31:0), LPC (0:0/20:0), and LPS (20:0/0:0] were used to distinguish different treated goat milk. Moreover, glycerophospholipid metabolism was the primary pathway of DLs. These results would provide more details of lipid profiles in thermally treated (pasteurization and spray drying) goat milk. [ABSTRACT FROM AUTHOR]

The aim of this study was to obtain dried powders from the brown, green and red varieties of the Brazilian propolis by spray drying, using gum arabic and maltodextrin as encapsulating materials. Propolis microparticles were evaluated for particle size, X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), moisture, water activity, solubility, hygroscopicity, encapsulation efficiency, total phenolic content, flavonoids and antioxidant activity (DPPH, ABTS + , FRAP, ORAC). Bioactive compounds were determined by UPHLC-QqQ-MS/MS. The results showed that the microparticles presented spherical forms, smooth surfaces, amorphous characteristics, low water activity, moisture and hygroscopicity, high solubility, good encapsulation efficiency, good antioxidant activity, although a reduction in total phenolics and flavonoids from the non-encapsulated propolis. The most important compounds in propolis powders were artepillin C, kaempferide, p -coumaric acid, luteolin, chlorogenic acid, kaempferol and caffeic acid. Spray-drying of propolis is a viable option to increase its use in food and pharmaceutical applications. [ABSTRACT FROM AUTHOR]

Yeast cells ( Saccharomyces cerevisiae ), from which β-glucans have been partially extracted, were used to encapsulate flavor inside the lipid bilayer membrane as natural encapsulant. The focus of this study was to investigate the release and stability of flavors ( d -limonene and ethyl hexanoate) encapsulated in yeast cells and maltodextrin (MD) (DE = 19) by spray drying. The release behavior of encapsulated flavors from yeast cells was measured at 40, 60, 80, and 105 °C with different moisture content (0, 50, 100, and 200% of powder). Water affected flavor release from the yeast cells. The release rate constants were correlated using Gaussian distribution of the activation energy of the release rate constants. The release of d -limonene from the spray-dried MD powder showed a different trend than that of yeast cells at various temperatures. The activation energies of the release rate constant for ethyl hexanoate and d -limonene from yeast were 55 and 49 kJ/mol, respectively, under a wet condition. The formation rates of limonene oxide and carvone were slower in yeast than that of MD powder at 30 °C after 2 months. [ABSTRACT FROM AUTHOR]

Few reports describe the effect of lactose hydrolysis on the properties of milk powder during production and storage. Hence, the aim of this study was to evaluate the effects of five different levels of enzymatic lactose hydrolysis during the production and storage of milk powder. As the lactose hydrolysis rate increased, adhesion to the drying chamber also increased, due to higher levels of particle agglomeration. Additionally, more brown powder was obtained when the lactose hydrolysis rate was increased, which in turn negatively affected rehydration ability. Using Raman spectroscopy, crystallization of the lactose residues in various samples was assessed over 6 weeks of accelerated aging at a room temperature environment with 75.5% of air moisture. Products with 25% or greater lactose hydrolysis showed no signs of crystallization, in contrast to the non-hydrolyzed sample. [ABSTRACT FROM AUTHOR]

Lipid peroxidation induced by oxidative stress plays an important role in reducing probiotic survival during spray drying. In this study, the probiotic Lactobacillus reuteri KUB-AC5 was co-encapsulated with holy basil essential oil, HBEO (0, 6, and 12 mg/ml), by spray drying using skim milk at inlet air temperatures of 130, 140, and 150 °C. The microcapsule properties in terms of morphology, size, moisture content, water activity, surface oil, microencapsulation efficiency, antioxidant activity, lipid peroxidation, and cell survival were determined. The result showed that the microcapsules with HBEO had much higher antioxidant activity than the ones without HBEO. The optimal condition of HBEO (6 mg/ml) with inlet temperature (130 °C) was effective in reducing malondialdehyde (MDA) level and enhancing survival of probiotics. This study indicates the potential role of HBEO in decreasing oxidative stress in probiotics during spray drying, which contributed to an increase in cell survival and health benefits. [ABSTRACT FROM AUTHOR]

[Display omitted] • Jet milling was used for the first time to prepare solid dispersions. • Solid dispersion technique was combined with cyclodextrin complexation technique. • The dispersion effect of jet milling was comparable to that of spray drying. • The antioxidant activity and cytotoxicity of cannabidiol were enhanced by dispersion. Jet milling is a common technique in ultrafine powder preparation field. It has never been used to design delivery systems. Cannabidiol (CBD) is an important cannabinoid of hemp but poor aqueous solubility limited its applications. In this study, solid dispersion (SD) technique was combined with cyclodextrin complexation technique, and jet milling was used for the first time to prepare SDs for improving CBD solubility. Different characterizations demonstrated that the dispersion effect and complexation structure of CBD SD3 prepared by jet milling were comparable to that of CBD SD2 prepared by spray drying (a common solution-based method), and were better than that of CBD SD1 prepared by cogrinding. The water solubility of CBD was increased to 20.902 μg/mL (909-fold) in CBD SD3. Besides, the antioxidant activity and cytotoxicity to tumor cells of CBD were enhanced by dispersion. This work indicated that jet milling, as a new technique with low cost and excellent applicability, could be further developed for the delivery of food functional factors or bioactive molecules. [ABSTRACT FROM AUTHOR]

[Display omitted] • Honey powder was produced using rice, pea or a mixture of both proteins as carriers. • Wet-bulb temperature was low enough to maintain the total phenolic compounds. • Honey powder hygroscopy values were satisfactory, even with high sugar content. • Honey powder can be an interesting ingredient to be applied in a real food matrix. Honey is a special product widely appreciated because of its peculiar flavor and aroma as well as its beneficial effects on health due to its constituents. However, the use of honey in its natural form can present several disadvantages to the food industry because of its high viscosity and density. This work aimed to obtain honey powder using rice, pea, or a mixture of both proteins as carriers by spray drying and to characterize physiochemically. Also, the mass balance was performed to evaluate changes in humidity and temperature that occurred by the drying air during the process. The honey showed acceptable physicochemical parameters by the legislation of honey quality control in regard to color (143.43 ± 4.34) mm Pfund, free acidity (46.41 ± 0.53) meq/kg, pH (3.73 ± 0.03), fructose content (46.52 ± 0.56) g/100 g and glucose content (35.88 ± 0.16) g/100 g, which leads to the production of honey powder. Among the carriers tested, the honey powder using rice protein achieved the highest powder recovery yield at (64.88 ± 0.64) %. The physicochemical properties were evaluated and the phenolic compounds were not negatively affected by spray drying conditions, maintaining a value of gallic acid equivalent (GAE) content at (301.31 ± 20.95) mg/kg of honey. Therefore, this work shows honey as an alternative food ingredient in powdered form, including the growing market for using alternative protein. [ABSTRACT FROM AUTHOR]

[Display omitted] • The encapsulation system of DHA encapsulated by natural egg yolk was developed. • More stable encapsulation structure formed by two-stage homogenization. • Two-stage homogenization promoted rupture and reassembly of droplets. • Vaccum low-temperature spray drying produced powder with high hydration properties. The use of safe physical means to achieve egg yolk as natural carrier for active ingredients plays an important role in increasing the added value of egg yolk. In this paper, we prepared DHA-fortified egg yolk emulsion using high-speed shearing (HSS) only and HSS combined with high-pressure homogenization (HPH), respectively. HPH reduced particle size and zeta potential, allowing for better emulsion stability. After 14 days of storage, the encapsulation efficiency was 93.88% even with 15% (w/w) algae oil addition. Rheology analysis presented that HPH improve the viscoelasticity, indicating the enhancement of interaction force between droplets. Then, vaccum low-temperature spray drying (VLTSD) was used to produce powder, which allowed for minimal damage to the encapsulation structure according to scanning electron microscopy and the hydration properties of powder was improved. This work provides a new idea for using egg yolk to encapsulate DHA and improving the properties of egg yolk powder. [ABSTRACT FROM AUTHOR]

[Display omitted] • L-menthol-based products generally show the flavor loss and the inadequate release. • SFD process produced the spherical microparticles with highest porosity of 86.82 %. • SFD microparticles enhanced the flavor retention during the high humidity storage. • The porous structures caused the rapid rehydration and flavor release in liquids. • The release behaviors of L-menthol in SFD powders followed the Fickian diffusion. L-menthol-containing food products generally show the flavor loss during storage due to their high volatility. The hydrophobicity of L-menthol also causes the inadequate flavor release during rehydration. In this study, the stability of L-menthol was enhanced by microencapsulation and the effect of different powder drying techniques was also investigated. The highest efficiency (76.58–78.66 %) and loading content (18.58–28.35 mg/g) of encapsulations were obtained by using a mass ratio of 2:1(α-LA: GA). Then they were dried by non-thermal spray freeze drying (SFD) technique compared to spray drying (SD) and freeze-drying (FD) process. The SFD particles were shown to be spherical and porous with the highest porosity (86.82 %). α-LA/GA based microparticles with spherical shapes were demonstrated to largely enhance flavor retention during high humidity storage. In addition, the porous structures of SFD powders could cause rapid rehydration in liquid models, and the release behaviors of loaded L-menthol followed the Fickian diffusion. Consequently, the SFD technique shows great potential to produce microparticles by regulating the release behaviors of L-menthol during storage and rehydration. [ABSTRACT FROM AUTHOR]

[Display omitted] • Modified WPI was prepared by heating, TG enzyme crosslinking and spray drying. • Modified WPI was self-gelation and stable in a simulated gastric environment. • Modified WPI achieved casein-like digestive rate and supplied more BCAA than casein. • Modified WPI consistently released BCAA into mice serum during 6 h in vivo digestion. Our study aimed to fabricate a modified slow-digestive whey protein isolate (WPI), which can supply enough branched-chain amino acids (BCAAs) during long-term fasting. The WPI aqueous solution (10 % w/v) was treated by heat (80 ℃) to unfold the protein tertiary structure, and subsequently treated with transglutaminase to form a gel via cross-linking. The powder of the WPI gel was obtained by spray drying, which can dissolve in water easily and self-assemble into gels again. This modified WPI contained protein aggregates with high molecular weight, and kept a stable gel-like structure under simulated gastric digestion conditions (pH = 3, 37 ℃). A dense honeycomb internal microstructure of the freeze-dried gel was observed. Further, we found that the WPI gel successfully achieved a casein-like digestible ratio (37.37 %) and released more BCAAs (0.18 mg/mL) than casein during the 4 h of in vitro simulated digestion based on the INFOGEST method. Finally, our results showed that the C57BL/6 mice oral administrated with the modified WPI gel had consistently higher BCAAs concentration (0.052 mg/mL) in their blood serum than the mice with normal WPI intake during the 6 h of in vivo digestion. [ABSTRACT FROM AUTHOR]

[Display omitted] • Spray drying was used to produce microparticulate fibers as a fat replacer. • Microparticles of dietary fiber has functional properties required for fatty sensation. • Microparticles of dietary fiber added in a fat-reduced model hazelnut spread. • Reduction of calories, total fat, and saturated fat was achieved. • Increase of 80% of dietary fiber was achived by using microparticles. The present work demonstrates the application of the spray drying technique to produce microparticulates of different dietary fibers with particle sizes<10 µm. It examines their role as potential fat replacers for hazelnut spread creams. Optimization of a dietary fiber formulation containing inulin, glucomannan, psyllium husk, and chia mucilage to obtain high viscosity, water holding capacity, and oil holding capacity was conducted. Microparticles containing 46.1, 46.2, and 7.6 weight percentages of chia seed mucilage, konjac glucomannan, and psyllium husk showed a spraying yield of 83.45 %, a solubility of 84.63 %, and viscosity of 40.49 Pas. When applied to hazelnut spread creams, microparticles substituted palm oil by 100 %; they produced a product with a total unsaturated and saturated fat reduction of 41 and 77 %, respectively. An increase in dietary fibers of 4 % and a decrease in total calories of 80 % were also induced when compared with the original formulation. Hazelnut spread with dietary fiber microparticles were preferred by 73.13 % of the panelist in the sensory study due to an enhancement in brightness. The demonstrated technique could be used to increase the fiber content while decreasing the fat content in some commercial products, such as peanut butter or chocolate cream. [ABSTRACT FROM AUTHOR]

[Display omitted] • Brazilian red propolis powdered was produced. • Spray-dried particles release formononetin in oral phase. • Spray-chilled particles release formononetin in intestinal phase. • Coated particles release formononetin gradually. • Microparticles protect red propolis bioactive compounds. Red propolis, originary from Northeast Brazil, has a unique composition and a great commercial interest. However, due to the presence of ethanol and its remarkable sensory characteristic, its application in food products is challenging. Thus, the aim of this work was to microencapsulate the red propolis extract by spray-drying, spray-chilling, and combining both techniques. The particles loaded with propolis extracts were characterised and evaluated according to the stability of phenolic compounds, flavonoids and formononetin, during 60 days of storage. In addition, the formononetin release was also monitored during the oral, gastric, and intestinal phases in the in vitro digestion process. All produced particles presented matrix-type with size, distribution, shape, hygroscopicity, and dispersibility parameters that varied according to the carrier and encapsulation process applied. The techniques used to fabricate the particles efficiently obtained powdered propolis extract and protected the extract's bioactive compounds, total flavonoids and formononetin throughout the analysed period. The gastrointestinal release study presented distinctive releases in all phases (oral, gastric, and intestinal). The spray-dried particles, for example, released formononetin mainly in the oral stage. While the spray-chilled particles were primarily released in the intestinal phase, and coated particles were released gradually throughout the assay, reaching maximum relief in the intestinal phase. In conclusion, using microencapsulation techniques by spray-drying, spray-chilling, and their combination developed particles with different levels of protection during storage, releases and characteristics, which resulted in a range of possible applications in the food, feed, cosmetic, and pharmaceutical industries. [ABSTRACT FROM AUTHOR]

[Display omitted] • Sweet potato flour was used for microencapsulation of probiotics. • Materials used promoted greater resistance to Lactiplantibacillus plantarum. • The microparticles proved to be a possible application in industry. • Probiotic showed counts above 8.0 log CFU/g in the gastrointestinal tract. Different plant products and co-products have been studied as wall materials for the microencapsulation of probiotics due to the need for new lost-cost, abundant, and natural materials. In this study, microparticles were developed by spray drying using different combinations of conventional materials such as maltodextrin, pectin, gelatin, and agar-agar with unconventional materials such as sweet potato flour to microencapsulate Lactiplantibacillus plantarum. The microparticles obtained were evaluated for encapsulation efficiency, thermal resistance, and rupture test. The most resistant microparticles were characterized and evaluated for probiotic viability during storage and survival to in vitro gastrointestinal conditions. Microparticles A (10 % maltodextrin, 5 % sweet potato flour, and 1 % pectin) and B (10 % maltodextrin, 4 % sweet potato flour, and 2 % gelatin) showed high thermal resistance (>59 %) and survival in acidic conditions (>80 %). L. plantarum in microparticles A and B remained viable with counts > 6 log CFU.g−1 for 45 days at 8 °C and −18 °C and resisted in vitro gastrointestinal conditions after processing with counts of 8.38 and 9.10 log CFU.g−1, respectively. Therefore, the selected microparticles have great potential for application in different products in the food industry, as they promote the protection and distribution of probiotic microorganisms. [ABSTRACT FROM AUTHOR]

[Display omitted] • Wood hemicelluloses (GGM and GX) were good wall materials to microencapsulate oil. • GGM and GX had much higher oil encapsulation efficiency (EE) than gum Arabic (GA). • Increasing solid ratios of GGM and GX to oil increased their EE. • GGM and GX protected oil from oxidation during spray drying better than GA. • GGM and GX microcapsules had spherical-shape particles with amorphous structure. The most commonly-used and effective wall materials (WMs) for spray-dried microencapsulation of bioactive compounds are either costly, or derived from unsustainable sources, which lead to an increasing demand for alternatives derived from sustainable and natural sources, with low calories and low cost. Wood hemicelluloses obtained from by-products of forest industries appear to be attractive alternatives as they have been reported to have good emulsifying properties, low viscosity at high concentrations, high heat stability and low heat transfer. Here, we investigated the applicability of spruce galactoglucomannans (GGM) and birch glucuronoxylans (GX), to encapsulate flaxseed oil (FO, polyunsaturated fatty acid-rich plant based oil) by spray drying; and the results were compared to those of the highly effective WM, gum Arabic (GA). It was found that depending on solid ratios of WM:FO (1:1, 3:1 and 5:1), encapsulation efficiency of GGM was 88–96%, and GX was 63–98%. At the same encapsulation ratio, both GGM and GX had higher encapsulation efficiency than GA (49–92%) due to their ability to produce feed emulsions with a smaller oil droplet size and higher physical stability. In addition, the presence of phenolic residues in GGM and GX powders enabled them to have a greater ability to protect oil from oxidation during spray drying than GA. Physiochemical properties of encapsulated powders including thermal properties, morphology, molecular structure, particle size and water adsorption intake are also investigated. The study has explored a new value-added proposition for wood hemicelluloses which can be used as effective WMs in the production of microcapsules of polyunsaturated fatty acid-rich oils for healthy and functional products in food, pharmaceutical and cosmetic industries. [ABSTRACT FROM AUTHOR]