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Abstract: Mamey sapote (Pouteria sapota (Jacq.) H. E. Moore & Stearn) is a tropical species from the Sapotaceae family native to Mexico and Central America. Its tree produces an edible climacteric fruit, whose weight can range from 250 to 900g. The flesh is soft, comprises about 78% of the fruit, and has high sugars to acidity ratio, which gives it a sweet taste when ripe. Because of current public interest to consume products that promote health, the aim of the work was to characterize soluble phenolic content and antioxidant activity in mamey sapote fruits from Chiapas, Mexico, at different maturity stages. Storage at 12 (±1)°C was conducted for 18d. When fruits ripened firmness flesh varied, in average, from 41.8 to 3.0N, soluble solids content from 17.7 to 28.1 ºBrix, hue angle from 56.4 to 46.3º, and lightness from 67.3 to 42.0. Phenolic content was affected by ripening since average values of 2563 and 234μg/g were found in unripe and consumption maturities, respectively. In a soluble phenolics extract from flesh, gallic acid (GA), gallocatechin-3-gallate (G3G), epicatechin (ECT), and catechin (C) were found, being the latter the most abundant compound, which increased with ripening from 9.9 to 113.1μg/g, while GA, G3G, and ECT had average values of 4.7, 11.9, and 5.8μg/g, respectively, without significant variation. Antioxidant activity, expressed through the IC50 parameter, remained practically unchanged and showed an average value of 12.9μg/mL. Based on phenolic composition the mamey sapote fruit may constitute a good source of antioxidant compounds. [Copyright &y& Elsevier]

Abstract: Phytochemical compounds in fruits and vegetables have gained great importance in the last few years because of the increasing evidence suggesting their antioxidant and prevention of chronic diseases. Carotenoids, phenolics, flavonoids, and vitamins E and C, are among these phytochemicals. Several fruits have been characterized so far for their antioxidant and health properties but there is still limited information on fruits from the tropic. Therefore, the objective of this study was the characterization of mamey fruit (Pouteria sapota Jacq. H. E. Moore & Stearn) with regard to their antioxidant capacity and phytochemical profile. Phenolics, carotenoids and δ-tocopherol were quantified and identified by HPLC–DAD–Mass Spectrometry (LC–MS), and DPPH and FRAP assays were used to evaluate antioxidant capacity. Hydrophilic extracts of mamey fruit showed higher antioxidant capacity than the lipophilic portion. Total soluble phenols content was 28.5mg GAE/100g fw, being p-hydroxybenzoic acid as the main phenolic that was identified. Total carotenoid content was 1127.9μg β-carotene/100g fw with β-carotene being the main contributor, in addition to lutein, and violoxanthin. Concentration of δ-tocopherol was 360.0μg/100g fw. Results of this study suggest that mamey fruit is a good source of carotenoids and its inclusion in the diet is recommended. [Copyright &y& Elsevier]