1. Identification of Variety of Glutinous Rice by RAPD Method
- Author
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Katsumi Takano, Yoshimasa Tsujii, Masataka Uchino, and Toru Yamagishi
- Subjects
chemistry.chemical_compound ,chemistry ,Botany ,food and beverages ,Food science ,Biology ,DNA ,RAPD - Abstract
In this study, we examined identification of glutinous rice breed by RAPD method. Seven polished rice, Hiyokumochi, Hakutyoumochi, Himenomoti, Koganemoti, Miyakoganemoti, Wataboushi, and Chinese rice were used in this study. In addition, these rices were produced in 2000. DNA was extracted and purified by CTAB method. DNA was amplified with OPB, OPM, and OPT primers (Operon Inc.) by PCR. Molecular weight of amplified DNA was determined by electrophoresis. As a result, all breeds of rice were determined by RAPD method.
- Published
- 2003
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