1. Phycocyanobilin-modified β-lactoglobulin exhibits increased antioxidant properties and stability to digestion and heating
- Author
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Andreja Rajkovic, Sam Van Haute, Mirjana Radomirovic, Simeon Minic, Dragana Stanic-Vucinic, Tanja Cirkovic Velickovic, and Milan Nikolić
- Subjects
Agriculture and Food Sciences ,WHEY PROTEINS ,Whey protein ,Antioxidant ,Antioxidant properties ,General Chemical Engineering ,medicine.medical_treatment ,Phycocyanobilin ,β-lactoglobulin ,01 natural sciences ,Adduct ,chemistry.chemical_compound ,Pigment ,fluids and secretions ,0404 agricultural biotechnology ,General chemistry ,COVALENT BINDING ,ANTIGENICITY ,medicine ,IGE ,ALLYL ISOTHIOCYANATE ,reproductive and urinary physiology ,C-PHYCOCYANIN ,2. Zero hunger ,PHYSICOCHEMICAL CHARACTERIZATION ,beta-lactoglobulin ,organic chemicals ,Functional food ,010401 analytical chemistry ,Heat aggregation ,food and beverages ,04 agricultural and veterinary sciences ,General Chemistry ,AGGREGATION ,040401 food science ,Combinatorial chemistry ,0104 chemical sciences ,stomatognathic diseases ,chemistry ,Covalent bond ,Digestibility ,visual_art ,RADICAL ABSORBENCY CAPACITY ,visual_art.visual_art_medium ,Digestion ,GLYCATION ,Food Science - Abstract
beta-lactoglobulin (BLG) is a major whey protein with numerous techno-functional properties desirable for the food industry. Phycocyanobilin (PCB), a bioactive pigment of Arthrospira platensis with health-promoting effects, covalently binds to BLG at physiological pH. This study investigated the effects of this covalent modification on BLG functional properties. The BLG-PCB adduct possesses enhanced antioxidant properties, and bound PCB protects BLG against free radical-induced oxidation. Despite the similar thermal stabilities of BLG and BLG-PCB, BLG-PCB is less susceptible to covalent and noncovalent aggregation under moderate heat treatment (63 degrees C, 30 min). Blocked thiol group and reduced hydrophobicity due to hindering of hydrophobic residues by bound PCB, as well as the heat-induced transition of beta-sheet to alpha-helix, contributed to the low susceptibility of BLG-PCB to aggregation. BLG-PCB has a higher resistance to pepsin and pancreatin digestion than BLG and unaltered IgEbinding properties. The improved functional properties of BLG-PCB make it a useful ingredient in the food industry.
- Published
- 2022
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