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Human exposure to pesticides commands the implementation of food safety control, but few studies have provided a comparative assessment of conventional and organic products. This study set out to examine 22 pesticides in four distinct commodities (lettuce, apples, grapes, and tomatoes) from conventional and organic agriculture available to consumers. A multiresidue procedure based on QuEChERS extraction and ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was first validated for its robustness. Suitable determination coefficients (R2 > 0.99) and recoveries generally between 70 and 110% were obtained. Intraday and interday variations were <20% and low matrix effects were noted with sample-to-sample variation. Method limits of detection (LOD) in the overall range of 0.05–2 μg kg−1 were obtained. The validated method was applied to 133 fruit and vegetable samples purchased in Canada, including conventional and organic culture samples. Overall, 47% of the 133 samples had levels above the LOD for at least one pesticide. Neonicotinoid insecticides were detected in all four product types. Imidacloprid (0.08–29 μg kg−1), acetamiprid (0.11–108 μg kg−1), and clothianidin (0.13–141 μg kg−1) were the most recurrent. Atrazine was reported in approximately a third of the lettuce samples (0.25–7.5 μg kg−1). For varieties with samples available from both organic and conventional agriculture, the proportion of insecticide levels > LOD was significantly higher (p < 0.05) for samples from conventional agriculture (9.7%) than from organic agriculture (2.0%). Measured levels were compliant to Canadian Maximum Residue Limits (MRLs), but a thorough human health risk assessment has yet to be conducted for many of these pesticides. • Multiclass pesticides were analyzed by QuEChERS–LC-MS/MS in fruits and vegetables. • Testing of 133 real samples indicated 47% of positives to at least one pesticide. • Multiple pesticide residues were detected in about half of these positive samples. • Recurrent pesticides were imidacloprid, acetamiprid, clothianidin, and atrazine. • Higher pesticide occurrence was noted in varieties of conventional vs. organic culture. [ABSTRACT FROM AUTHOR]