1. Integrated network pharmacology and cellular assay for the investigation of an anti-obesity effect of 6-shogaol
- Author
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Wenya Jiao, Qiuxia Jin, Shuo Wang, Yaxin Sang, Si Mi, Bimal Chitrakar, and Xianghong Wang
- Subjects
Cell ,Catechols ,AKT1 ,Network Pharmacology ,Pharmacology ,Analytical Chemistry ,Mice ,Phosphatidylinositol 3-Kinases ,chemistry.chemical_compound ,3T3-L1 Cells ,Lipid droplet ,Adipocytes ,medicine ,Animals ,Obesity ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Adipogenesis ,Cellular Assay ,General Medicine ,Shogaol ,Molecular Docking Simulation ,medicine.anatomical_structure ,chemistry ,Phosphorylation ,Food Science - Abstract
This study explored the anti-obesity effect of 6-shogaol and the underlying mechanisms by using Network pharmacology for the prediction and verification of molecular targets and pathways of 6-shogaol against obesity. Furthermore, the results were verified by molecular docking and cell experiments. A total of 86 core targets of 6-shogaol towards obesity were identified. Among them, AKT1 and PIK3CA were confirmed by using the molecular docking. In 3T3-L1 preadipocyte model, 6-shogaol significantly inhibited proliferation and differentiation, reducing the accumulation of lipid droplets. Compared with the control group, the inhibition rates of 6-shogaol on TG and TC were 90.8% and 40.0%, respectively. Additionally, 6-shogaol down-regulated the expression of PPAR-γ and C/EBP-α, while it decreased the phosphorylation of IRS-1, PI3K and AKT. This study, for the first time, confirmed the effect of 6-shogaol on improving obesity through PI3K/AKT pathway. An anti-obesity bioactivity study was further recommended for the development of novel anti-obesity products.
- Published
- 2022
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